Product nameAnti-Hsp40 antibody
See all Hsp40 primary antibodies
DescriptionRabbit polyclonal to Hsp40
SpecificityThis antibody detects a ~40 kDa protein, corresponding to the apparent molecular mass of Hsp40 on SDS-PAGE immunoblots. It does not detect DnaJ in E.coli lysates.
Tested applicationsSuitable for: WB, IP, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Fish, Monkey
Does not react with: Escherichia coli
Recombinant human Hsp40
- HeLa heat shocked cell lysate and recombinant human Hsp40 Protein
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab69402 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa).|
|ICC/IF||1/100 - 1/400.|
FunctionInteracts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP.
Sequence similaritiesContains 1 J domain.
Cellular localizationCytoplasm. Nucleus. Nucleus > nucleolus. Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock.
- Information by UniProt
- DnaJ (Hsp40) homolog subfmaily B member 1 antibody
- DNAJ 1 antibody
- DNAJ B1 antibody
All lanes : Anti-Hsp40 antibody (ab69402) at 1/1000 dilution
Lane 1 : Molecular weight marker
Lane 2 : Hsp40 recombinant protein
Lane 3 : DnaJ recombinant protein
Lane 4 : Cell lysates prepared from heat shocked hela cells
Lane 5 : Cell lysates prepared from heat shocked 3T3 cells
Lane 6 : Cell lysates prepared from PC-12 cells
Predicted band size: 38 kDa
ab69402 staining Hsp40 in the A549 human lung cancer cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% and blocked with 5% BSA for 20 minutes at 20°C. Samples were incubated with primary antibody (1/200 in PBS) for 1 hour at 20°C. A CF568-conjugated Donkey anti-rabbit IgG (H+L) polyclonal was used as the secondary antibody.
This product has been referenced in:
- Park SK et al. Overexpression of the essential Sis1 chaperone reduces TDP-43 effects on toxicity and proteolysis. PLoS Genet 13:e1006805 (2017). ICC/IF ; Rat . Read more (PubMed: 28531192) »
- Weber TA et al. APOOL is a cardiolipin-binding constituent of the Mitofilin/MINOS protein complex determining cristae morphology in mammalian mitochondria. PLoS One 8:e63683 (2013). WB ; Human . Read more (PubMed: 23704930) »