Name: Anti-Hsp47 antibody [EPR4217]
Brand: RabMAb
SKU: ab109117
Rating: 5 (5 reviews)

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Primary - Rabbit Anti-Hsp47 antibody [EPR4217] (ab109117)
WB, IHC-P

Secondary - Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
IHC-P, WB, ELISA, IP

Protein - Recombinant Human Hsp47 protein (ab182711)
SDS-PAGE

View more associated products

Product name

Anti-Hsp47 antibody [EPR4217]
See all Hsp47 primary antibodies
Description

Rabbit monoclonal [EPR4217] to Hsp47
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-Pmore details
Species reactivity

Reacts with: Mouse, Human
Predicted to work with: Rat
Immunogen

Synthetic peptide within Human Hsp47 aa 400-500. The exact sequence is proprietary.
Positive control
- HeLa, A431 and NIH/3T3 cell lysates; Human breast tissue; HeLa cells
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4217
Isotype

IgG
Research areas

Alternative Versions
- Anti-Hsp47 antibody [EPR4217] (HRP) (ab193242)
- Anti-Hsp47 antibody [EPR4217] - BSA and Azide free (ab226052)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human Hsp47 protein (ab182711)

Our Abpromise guarantee covers the use of ab109117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application

Abreviews

Notes

1/1000 - 1/10000. Predicted molecular weight: 46 kDa.

For unpurified use at 1/100.

IHC-P

1/300. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/100 - 1/250.

Function

Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
Involvement in disease

Note=Defects in SERPINH1 may cause severe autosomal recessive osteogenesis imperfecta (OI). Osteogenesis imperfecta defines a group of connective tissue disorders characterized by bone fragility and low bone mass.
Sequence similarities

Belongs to the serpin family.
Cellular localization

Endoplasmic reticulum lumen.
Target information above from: UniProt accession P50454 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 871 Human
- Entrez Gene: 12406 Mouse
- Entrez Gene: 29345 Rat
- Omim: 600943 Human
- SwissProt: P50454 Human
- SwissProt: P19324 Mouse
- SwissProt: P29457 Rat
- Unigene: 596449 Human
- Unigene: 22708 Mouse
- Unigene: 444951 Mouse
- Unigene: 98199 Rat
see all
Alternative names
- 47 kDa heat shock protein antibody
- 47 kDa heat shock protein precursor antibody
- Arsenic transactivated protein 3 antibody
- Arsenic-transactivated protein 3 antibody
- AsTP 3 antibody
- AsTP3 antibody
- CBP 1 antibody
- CBP 2 antibody
- CBP1 antibody
- CBP2 antibody
- Cell proliferation-inducing gene 14 protein antibody
- Collagen binding protein 1 antibody
- Collagen binding protein 2 antibody
- Collagen binding protein antibody
- Collagen-binding protein 2 antibody
- Collagen-binding protein antibody
- Colligen antibody
- Colligin 1 antibody
- Colligin 2 antibody
- Colligin antibody
- colligin-1 antibody
- colligin-2 antibody
- gp46 antibody
- Heat shock protein 47 antibody
- Heat-shock protein 47 antibody
- HGNC 1547 antibody
- Hsp 47 antibody
- J6 antibody
- OI10 antibody
- PIG 14 antibody
- PIG14 antibody
- PPROM antibody
- Proliferation inducing gene 14 antibody
- Proliferation inducing gene 14 protein antibody
- RA A47 antibody
- RA-A47 antibody
- Rheumatoid arthritis antigen A 47 antibody
- rheumatoid arthritis antigen A-47 antibody
- Rheumatoid arthritis related antigen RA A47 antibody
- Rheumatoid arthritis-related antigen RA-A47 antibody
- serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) antibody
- serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 2, (collagen-binding protein 2) antibody
- Serine or cysteine proteinase inhibitor clade H member 1 antibody
- Serine or cysteine proteinase inhibitor clade H member 2 antibody
- SERPH_HUMAN antibody
- Serpin H1 antibody
- Serpin peptidase inhibitor clade H member 1 antibody
- serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) antibody
- Serpin peptidase inhibitor, clade H, member 1 antibody
- SERPINH1 antibody
- SERPINH2 antibody
see all

Western blot - Anti-Hsp47 antibody [EPR4217] (ab109117)

Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Hsp47 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HepG2 whole cell lysate (20 µg)

Lanes 1 - 4: Merged signal (red and green). Green - ab109117 observed at 46 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab109117 was shown to recognize Hsp47 in wild-type HAP1 cells as signal was lost at the expected MW in Hsp47 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Hsp47 knockout samples were subjected to SDS-PAGE. ab109117 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp47 antibody [EPR4217] (ab109117)

ab109117 staining Hsp47 in Human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/300). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin.
Western blot - Anti-Hsp47 antibody [EPR4217] (ab109117)

All lanes : Anti-Hsp47 antibody [EPR4217] (ab109117) at 1/2000 dilution

Lane 1 : A431 Cell Lysate
Lane 2 : NIH/3T3 Cell Lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

Predicted band size: 46 kDa
Western blot - Anti-Hsp47 antibody [EPR4217] (ab109117)

All lanes : Anti-Hsp47 antibody [EPR4217] (ab109117) at 1/1000 dilution (unpurified)

Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate
Lane 3 : NIH/3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 46 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp47 antibody [EPR4217] (ab109117)

ab109117, unpurified, at 1/100 dilution, staining Hsp47 in Human breast tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot protocols
Immunohistochemistry protocols

This product has been referenced in:

Ohigashi H et al. Ocular instillation of vitamin A-coupled liposomes containing HSP47 siRNA ameliorates dry eye syndrome in chronic GVHD. Blood Adv 3:1003-1010 (2019). Read more (PubMed: 30940635) »
Wong R et al. Angiogenesis and tissue formation driven by an arteriovenous loop in the mouse. Sci Rep 9:10478 (2019). Read more (PubMed: 31324837) »

See all 12 Publications for this product

Publishing research using ab109117? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-7 of 7 Abreviews or Q&A

Application

Western blot

Sample

Mouse Tissue lysate - whole (Skeletal muscle)

Gel Running Conditions

Reduced Denaturing (12%)

Loading amount

20 µg

Specification

Skeletal muscle

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 15 2016

Application

Western blot

Sample

Rat Tissue lysate - whole (Skeletal muscle)

Gel Running Conditions

Reduced Denaturing (12%)

Loading amount

20 µg

Specification

Skeletal muscle

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 15 2016

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Lung tissue: fibroblasts)

Antigen retrieval step

None

Permeabilization

Specification

Lung tissue: fibroblasts

Blocking step

Zytomed Systems HRP-Polymer Kit Blocking Reagent as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C

Fixative

HOPE-Fixation

Abcam user community

Verified customer

Submitted Apr 13 2015

Question

Can you tell me what is the concentration from lot YI081905CS?

Abcam community

Verified customer

Asked on Jul 12 2013

Answer

The concentration for lot YI081905CS is 2.0130 mg/ml.

達偉鄭

Abcam Scientific Support

Answered on Jul 12 2013

Question

Could you let me know the concentration of the antibody linked below? https://www.abcam.com/Hsp47-antibody-EPR4217-ab109117.html

Abcam community

Verified customer

Asked on Oct 22 2012

Answer

Thank you for your enquiry.

I can confirm that ab109117anti-HSP47 antibody [EPR4217] is sold as tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datasheet. Antibody concentration is usually determined by protein assay, and tissue culture supernatant will contain a lot of other proteins, which means the antibody quantification would not be accurate.

I can confirm that for tissue culture supernatant, concentration of antibody is known to very between 1 - 3 mg/ml

I am sorry we are not able to provide an exact concentration on this occasion, but hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

Abcam Scientific Support

Answered on Oct 22 2012

Application

Western blot

Sample

Mouse Cell lysate - whole cell (chondrocyte cell line ATDC5)

Loading amount

30 µg

Specification

chondrocyte cell line ATDC5

Gel Running Conditions

Reduced Denaturing (4-20% Bis-Tris)

Blocking step

Milk as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jun 12 2012

Application

Western blot

Sample

Human Cell lysate - whole cell (hVSMCs)

Loading amount

5 µg

Specification

hVSMCs

Gel Running Conditions

Reduced Denaturing (10 %)

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 17 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

Applications

Target

Function

Involvement in disease

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

References

Customer reviews and Q&As

Western blot abreview for Anti-Hsp47 antibody [EPR4217]

Western blot abreview for Anti-Hsp47 antibody [EPR4217]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Hsp47 antibody [EPR4217]

Western blot abreview for Anti-Hsp47 antibody [EPR4217]

Western blot abreview for Anti-Hsp47 antibody [EPR4217]