Recombinant Anti-Hsp47 antibody [EPR4217] (ab109117)
![Western blot - Anti-Hsp47 antibody [EPR4217] (ab109117)](https://www.abcam.com/ps/products/109/ab109117/Images/ab109117-299856-anti-hsp47-antibody-epr4217-western-blot.jpg "Western blot - Anti-Hsp47 antibody [EPR4217] (ab109117)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4217] to Hsp47
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Hsp47 antibody [EPR4217]
See all Hsp47 primary antibodies -
Description
Rabbit monoclonal [EPR4217] to Hsp47 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, A431 and NIH/3T3 cell lysates; Human breast tissue; HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4217 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109117 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (4) |
1/1000 - 1/10000. Predicted molecular weight: 46 kDa.Can be blocked with Hsp47 peptide (ab283675).
For unpurified use at 1/100. |
| IHC-P | (1) |
1/300. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250. |
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 46 kDa.Can be blocked with Hsp47 peptide (ab283675). For unpurified use at 1/100. |
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IHC-P
1/300. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250. |
Target
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Function
Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen. -
Involvement in disease
Note=Defects in SERPINH1 may cause severe autosomal recessive osteogenesis imperfecta (OI). Osteogenesis imperfecta defines a group of connective tissue disorders characterized by bone fragility and low bone mass. -
Sequence similarities
Belongs to the serpin family. -
Cellular localization
Endoplasmic reticulum lumen. - Information by UniProt
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Database links
- Entrez Gene: 871 Human
- Entrez Gene: 12406 Mouse
- Entrez Gene: 29345 Rat
- Omim: 600943 Human
- SwissProt: P50454 Human
- SwissProt: P19324 Mouse
- SwissProt: P29457 Rat
- Unigene: 596449 Human
see all -
Alternative names
- 47 kDa heat shock protein antibody
- 47 kDa heat shock protein precursor antibody
- Arsenic transactivated protein 3 antibody
see all
Images
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Western blot - Anti-Hsp47 antibody [EPR4217] (ab109117)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Hsp47 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HepG2 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab109117 observed at 46 kDa. Red - loading control, ab9484, observed at 37 kDa.ab109117 was shown to recognize Hsp47 in wild-type HAP1 cells as signal was lost at the expected MW in Hsp47 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Hsp47 knockout samples were subjected to SDS-PAGE. ab109117 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp47 antibody [EPR4217] (ab109117)](https://www.abcam.com/ps/products/109/ab109117/Images/ab109117-239847-anti-hsp47-antibody-epr4217-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp47 antibody [EPR4217] (ab109117)ab109117 staining Hsp47 in Human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/300). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin.
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![Western blot - Anti-Hsp47 antibody [EPR4217] (ab109117)](https://www.abcam.com/ps/products/109/ab109117/Images/ab109117-239846-anti-hsp47-antibody-epr4217-western-blot.jpg)
Western blot - Anti-Hsp47 antibody [EPR4217] (ab109117)All lanes : Anti-Hsp47 antibody [EPR4217] (ab109117) at 1/2000 dilution
Lane 1 : A431 Cell Lysate
Lane 2 : NIH/3T3 Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution
Predicted band size: 46 kDa -
![Western blot - Anti-Hsp47 antibody [EPR4217] (ab109117)](https://www.abcam.com/ps/products/109/ab109117/Images/ab109117-121696-anti-hsp47-antibody-epr4217-western-blot.jpg)
Western blot - Anti-Hsp47 antibody [EPR4217] (ab109117)All lanes : Anti-Hsp47 antibody [EPR4217] (ab109117) at 1/1000 dilution (unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate
Lane 3 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 46 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp47 antibody [EPR4217] (ab109117)](https://www.abcam.com/ps/products/109/ab109117/Images/ab109117-121695-anti-hsp47-antibody-epr4217-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp47 antibody [EPR4217] (ab109117)ab109117, unpurified, at 1/100 dilution, staining Hsp47 in Human breast tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-Hsp47 antibody [EPR4217] (ab109117)](https://www.abcam.com/ps/products/109/ab109117/Images/ab109117-7-benefits-of-recombinant-antibodies.png)
Anti-Hsp47 antibody [EPR4217] (ab109117)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (30)
ab109117 has been referenced in 30 publications.
- Xu H et al. Inhibition of the mevalonate pathway improves myocardial fibrosis. Exp Ther Med 21:224 (2021). PubMed: 33603833
- Ruigrok MJR et al. Silencing Heat Shock Protein 47 (HSP47) in Fibrogenic Precision-Cut Lung Slices: A Surprising Lack of Effects on Fibrogenesis? Front Med (Lausanne) 8:607962 (2021). PubMed: 33659262
- Ma W et al. HSP47 contributes to angiogenesis by induction of CCL2 in bladder cancer. Cell Signal 85:110044 (2021). PubMed: 34000383
- Fan Q et al. Implantable blood clot loaded with BMP-2 for regulation of osteoimmunology and enhancement of bone repair. Bioact Mater 6:4014-4026 (2021). PubMed: 33997490
- Zhou R et al. miR-29a is a potential protective factor for fibrogenesis in gluteal muscle contracture. Physiol Res 69:467-479 (2020). PubMed: 32469233