Overview

  • Product name

    Anti-Hsp47 antibody [EPR4217]
    See all Hsp47 primary antibodies
  • Description

    Rabbit monoclonal [EPR4217] to Hsp47
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Human Hsp47 aa 400-500. The exact sequence is proprietary.

  • Positive control

    • HeLa, A431 and NIH/3T3 cell lysates; Human breast tissue; HeLa cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR4217
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 46 kDa.

For unpurified use at 1/100.

IHC-P 1/300. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/100 - 1/250.

Target

  • Function

    Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
  • Involvement in disease

    Note=Defects in SERPINH1 may cause severe autosomal recessive osteogenesis imperfecta (OI). Osteogenesis imperfecta defines a group of connective tissue disorders characterized by bone fragility and low bone mass.
  • Sequence similarities

    Belongs to the serpin family.
  • Cellular localization

    Endoplasmic reticulum lumen.
  • Information by UniProt
  • Database links

  • Alternative names

    • 47 kDa heat shock protein antibody
    • 47 kDa heat shock protein precursor antibody
    • Arsenic transactivated protein 3 antibody
    • Arsenic-transactivated protein 3 antibody
    • AsTP 3 antibody
    • AsTP3 antibody
    • CBP 1 antibody
    • CBP 2 antibody
    • CBP1 antibody
    • CBP2 antibody
    • Cell proliferation-inducing gene 14 protein antibody
    • Collagen binding protein 1 antibody
    • Collagen binding protein 2 antibody
    • Collagen binding protein antibody
    • Collagen-binding protein 2 antibody
    • Collagen-binding protein antibody
    • Colligen antibody
    • Colligin 1 antibody
    • Colligin 2 antibody
    • Colligin antibody
    • colligin-1 antibody
    • colligin-2 antibody
    • gp46 antibody
    • Heat shock protein 47 antibody
    • Heat-shock protein 47 antibody
    • HGNC 1547 antibody
    • Hsp 47 antibody
    • J6 antibody
    • OI10 antibody
    • PIG 14 antibody
    • PIG14 antibody
    • PPROM antibody
    • Proliferation inducing gene 14 antibody
    • Proliferation inducing gene 14 protein antibody
    • RA A47 antibody
    • RA-A47 antibody
    • Rheumatoid arthritis antigen A 47 antibody
    • rheumatoid arthritis antigen A-47 antibody
    • Rheumatoid arthritis related antigen RA A47 antibody
    • Rheumatoid arthritis-related antigen RA-A47 antibody
    • serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) antibody
    • serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 2, (collagen-binding protein 2) antibody
    • Serine or cysteine proteinase inhibitor clade H member 1 antibody
    • Serine or cysteine proteinase inhibitor clade H member 2 antibody
    • SERPH_HUMAN antibody
    • Serpin H1 antibody
    • Serpin peptidase inhibitor clade H member 1 antibody
    • serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) antibody
    • Serpin peptidase inhibitor, clade H, member 1 antibody
    • SERPINH1 antibody
    • SERPINH2 antibody
    see all

Images

  • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: Hsp47 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: HepG2 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab109117 observed at 46 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab109117 was shown to recognize Hsp47 in wild-type HAP1 cells as signal was lost at the expected MW in Hsp47 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Hsp47 knockout samples were subjected to SDS-PAGE. ab109117 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • ab109117 staining Hsp47 in Human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/300). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin.

  • All lanes : Anti-Hsp47 antibody [EPR4217] (ab109117) at 1/2000 dilution

    Lane 1 : A431 Cell Lysate
    Lane 2 : NIH/3T3 Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

    Predicted band size: 46 kDa

  • All lanes : Anti-Hsp47 antibody [EPR4217] (ab109117) at 1/1000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 46 kDa

  • ab109117, unpurified, at 1/100 dilution, staining Hsp47 in Human breast tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

References

This product has been referenced in:

  • Ohigashi H  et al. Ocular instillation of vitamin A-coupled liposomes containing HSP47 siRNA ameliorates dry eye syndrome in chronic GVHD. Blood Adv 3:1003-1010 (2019). Read more (PubMed: 30940635) »
  • Goldmann T  et al. Human alveolar epithelial cells type II are capable of TGFß-dependent epithelial-mesenchymal-transition and collagen-synthesis. Respir Res 19:138 (2018). Read more (PubMed: 30041633) »
See all 11 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 15 2016

Application
Western blot
Sample
Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 15 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Lung tissue: fibroblasts)
Antigen retrieval step
None
Permeabilization
No
Specification
Lung tissue: fibroblasts
Blocking step
Zytomed Systems HRP-Polymer Kit Blocking Reagent as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative
HOPE-Fixation

Abcam user community

Verified customer

Submitted Apr 13 2015

Question
Answer

The concentration for lot YI081905CS is 2.0130 mg/ml.

Read More

Answer

Thank you for your enquiry.

I can confirm that ab109117anti-HSP47 antibody [EPR4217] is sold as tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datasheet. Antibody concentration is usually determined by protein assay, and tissue culture supernatant will contain a lot of other proteins, which means the antibody quantification would not be accurate.

I can confirm that for tissue culture supernatant, concentration of antibody is known to very between 1 - 3 mg/ml

I am sorry we are not able to provide an exact concentration on this occasion, but hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

Read More
Application
Western blot
Sample
Mouse Cell lysate - whole cell (chondrocyte cell line ATDC5)
Loading amount
30 µg
Specification
chondrocyte cell line ATDC5
Gel Running Conditions
Reduced Denaturing (4-20% Bis-Tris)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jun 12 2012

Application
Western blot
Sample
Human Cell lysate - whole cell (hVSMCs)
Loading amount
5 µg
Specification
hVSMCs
Gel Running Conditions
Reduced Denaturing (10 %)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 17 2011

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