Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4217] to Hsp47 (HRP)
- Suitable for: IHC-P, WB
- Reacts with: Mouse
- Conjugation: HRP
Product nameAnti-Hsp47 antibody [EPR4217] (HRP)
See all Hsp47 primary antibodies
DescriptionRabbit monoclonal [EPR4217] to Hsp47 (HRP)
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Hsp47 aa 400 to the C-terminus.
- WB: NIH/3T3 whole cell lysate. IHC-P: normal human placenta tissue.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab193242 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/5000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).|
FunctionBinds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
Involvement in diseaseNote=Defects in SERPINH1 may cause severe autosomal recessive osteogenesis imperfecta (OI). Osteogenesis imperfecta defines a group of connective tissue disorders characterized by bone fragility and low bone mass.
Sequence similaritiesBelongs to the serpin family.
Cellular localizationEndoplasmic reticulum lumen.
- Information by UniProt
- 47 kDa heat shock protein antibody
- 47 kDa heat shock protein precursor antibody
- Arsenic transactivated protein 3 antibody
IHC image of Hsp47 staining in a section of formalin-fixed paraffin-embedded normal human placenta tissue*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab193242 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Anti-Hsp47 antibody [EPR4217] (HRP) (ab193242) at 1/5000 dilution + NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 46 kDa
Exposure time: 4 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab193242 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab193242 has not yet been referenced specifically in any publications.