Product nameHSP47 Antibody Pair - BSA and Azide free
See all Hsp47 kits
Assay typeELISA set
Range218.75 pg/ml - 14000 pg/ml
The Antibody Pair can be used to quantify HSP47. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab270882), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
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Tested applicationsSuitable for: ELISAmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components 10 x 96 tests HSP47 Capture Antibody (unconjugated) 1 x 100µg HSP47 Detector Antibody (unconjugated) 1 x 100µg
FunctionBinds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
Involvement in diseaseNote=Defects in SERPINH1 may cause severe autosomal recessive osteogenesis imperfecta (OI). Osteogenesis imperfecta defines a group of connective tissue disorders characterized by bone fragility and low bone mass.
Sequence similaritiesBelongs to the serpin family.
Cellular localizationEndoplasmic reticulum lumen.
- Information by UniProt
- 47 kDa heat shock protein
- 47 kDa heat shock protein precursor
- Arsenic transactivated protein 3
Our Abpromise guarantee covers the use of ab253318 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab253318 has not yet been referenced specifically in any publications.