• Product name

    Anti-Hsp60 antibody [1D11BD8] - Mitochondrial Marker
    See all Hsp60 primary antibodies
  • Description

    Mouse monoclonal [1D11BD8] to Hsp60 - Mitochondrial Marker
  • Host species

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, In-Cell ELISA, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Other Immunogen Type. This information is considered to be commercially sensitive.

  • Positive control

    • Human heart tissue HepG2 Fibroblast cells HeLa cells Human normal colon FFPE tissue.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.



Our Abpromise guarantee covers the use of ab110312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 5 µg/ml. Predicted molecular weight: 61 kDa.
ICC/IF Use a concentration of 1 µg/ml.
In-Cell ELISA Use a concentration of 1 µg/ml.
Flow Cyt Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • Function

    Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
  • Involvement in disease

    Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
    Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life.
  • Sequence similarities

    Belongs to the chaperonin (HSP60) family.
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • 60 kDa chaperonin antibody
    • 60 kDa heat shock protein, mitochondrial antibody
    • CH60_HUMAN antibody
    • Chaperonin 60 antibody
    • Chaperonin, 60-KD antibody
    • CPN60 antibody
    • fa04a05 antibody
    • GROEL antibody
    • heat shock 60kDa protein 1 (chaperonin) antibody
    • Heat shock protein 1 (chaperonin) antibody
    • Heat shock protein 60 antibody
    • Heat shock protein 65 antibody
    • heat shock protein family D (Hsp60) member 1 antibody
    • HLD4 antibody
    • Hsp 60 antibody
    • HSP 65 antibody
    • HSP-60 antibody
    • HSP60 antibody
    • HSP65 antibody
    • HSPD1 antibody
    • HuCHA60 antibody
    • Mitochondrial matrix protein P1 antibody
    • P60 lymphocyte protein antibody
    • short heat shock protein 60 Hsp60s1 antibody
    • SPG13 antibody
    see all


  • Anti-Hsp60 antibody [1D11BD8] - Mitochondrial Marker (ab110312) at 5 µg/ml + HepG2 whole cells at 10 µg

    Predicted band size: 61 kDa

  • ab110312 staining Hsp60 in MDA MB 231 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 1% Triton X-100 and blocked with 10% BSA for 1 hour at 21°C. Samples were incubated with primary antibody (1/100 in BSA + 0.02% Tween20) for 1 hour at 16°C. A DyLight® 550-conjugated goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Immunocytochemistry image of stained fibroblast cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with the ab110312 antibody (1 µg/mL) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent.
  • HeLa cells were stained with 1 µg/mL ab110312 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
  • IHC image of Hsp60 staining in Human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110312, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times


This product has been referenced in:

  • Liang XH  et al. RNase H1-Dependent Antisense Oligonucleotides Are Robustly Active in Directing RNA Cleavage in Both the Cytoplasm and the Nucleus. Mol Ther 25:2075-2092 (2017). Read more (PubMed: 28663102) »
  • Magnoni R  et al. The Hsp60 folding machinery is crucial for manganese superoxide dismutase folding and function. Free Radic Res 48:168-79 (2014). Read more (PubMed: 24151936) »
See all 2 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Human Cell (MDA MB 231 cells)
MDA MB 231 cells
Yes - 1% Triton

Abcam user community

Verified customer

Submitted Feb 19 2015


Thank you for your enquiry and your interest in our products.
As far as we understand the epitipe has not been mapped yet so unfortunately I can't provide this information for you. ab110312 has been tested in several applications (IHC-P, WB, ICC/IF, In-Cell ELISA, Flow Cyt) but not in IP so Abcam can't guarantee it.
If you wish to test it in IP, I could offer you a testing discount for you. I would advise you to visit the following site where the Terms and Conditions of our testing initiative can be found at: www.abcam.com/abtrial.
If you need any further assistance in the future, please do not hesitate to contact me.

Read More


I'm happy to help! Please feel free to contact me if you need anything further.

Read More


There are lots of enzyme functions going on in the mitochondrial matrix. So lots of different options for WB in the matrix:

MCAD – human and rodent http://www.mitosciences.com/mcad.html

PDH E1a – human and rodent https://www.abcam.com/Pyruvate-Dehydrogenase-E1-alpha-subunit-antibody-8D10E6-ab110334.html

OGDH E2 subunit – human only https://www.abcam.com/DLST-antibody-9F4BD5-ab110306.html

mtTFA – human only https://www.abcam.com/mtTFA-antibody-18G102B2E11-ab119684.html

SOD2 poly https://www.abcam.com/SOD2-antibody-ab13533.html

Aconitase rabbit poly https://www.abcam.com/Aconitase-2-antibody-ab71440.html

Citrate synthase https://www.abcam.com/Citrate-synthetase-antibody-ab96600.html
I hope this is helpful. Please contact me again if you have any further questions.

Read More

For licensing inquiries, please contact partnerships@abcam.com

Sign up