Product nameAnti-Hsp60 antibody
See all Hsp60 primary antibodies
DescriptionRabbit polyclonal to Hsp60
Tested applicationsSuitable for: ICC/IF, WB, IP, ELISA, IHC-P, IHC-Frmore details
Species reactivityReacts with: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Human
Recombinant full length Human Hsp60 protein produced in E. coli.
- Heat shocked HeLa cell lysate; recombinant Human HSP60, SKBR3, MDCK and MEF lysates
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab109660 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 61 kDa).|
|IP||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
FunctionImplicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
Involvement in diseaseDefects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life.
Sequence similaritiesBelongs to the chaperonin (HSP60) family.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- 60 kDa chaperonin antibody
- 60 kDa heat shock protein, mitochondrial antibody
- CH60_HUMAN antibody
Immunocytochemistry/Immunofluorescence analysis of Heat Shocked HeLa Cells labeling Hsp60 with ab109660 at 1/100 dilution for 12 hours at 4°C. Cells were fixed in 2% formaldehyde for 20 min at RT. A FITC conjugated Goat Anti-Rabbit secondary antibody (green) was used at 1/200 for 2 hours at RT. DAPI (blue) nuclear counter stain at 1/40000 for 2 hours at RT. Localization in the Mitochondrion matrix observed. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp60 Antibody. (C) Composite.
All lanes : Anti-Hsp60 antibody (ab109660) at 1 µg/ml
Lane 1 : Recombinant Human Hsp60 at 0.1 µg
Lane 2 : SKBR3 lysate (Human) at 7.5 µg
Lane 3 : MDCK lysate (Dog) at 7.5 µg
Lane 4 : MEF lysate (Mouse) at 7.5 µg
Predicted band size: 61 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Immunocytochemistry/Immunofluorescence analysis of Heat Shocked HeLa Cells labeling Hsp60 with ab109660 at 1/100 dilution for 12 hours at 4°C. Cells were fixed in 2% formaldehyde for 20 min at RT. APC Goat Anti-Rabbit (red) secondary antibody was used at 1/200 for 2 hours at RT. DAPI (blue) nuclear counter stain at 1/40000 for 2 hours at RT. Localization in the Mitochondrion matrix observed. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp60 Antibody. (C) Composite.
ab109660 has not yet been referenced specifically in any publications.