Anti-Hsp60 antibody (ab216634)
Key features and details
- Rabbit polyclonal to Hsp60
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Hsp60 antibody
See all Hsp60 primary antibodies -
Description
Rabbit polyclonal to Hsp60 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide aa 520-573 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: P10809 -
Positive control
- Human lung carcinoma, rat brain and rat stomach tissues; MCF7, mouse heart, mouse kideny and mouse liver lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab216634 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/100 - 1/1000. Predicted molecular weight: 61 kDa.
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Notes |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/100 - 1/1000. Predicted molecular weight: 61 kDa. |
Target
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Function
Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. -
Involvement in disease
Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life. -
Sequence similarities
Belongs to the chaperonin (HSP60) family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 3329 Human
- Entrez Gene: 15510 Mouse
- Entrez Gene: 63868 Rat
- Omim: 118190 Human
- SwissProt: P10809 Human
- SwissProt: P63038 Mouse
- SwissProt: P63039 Rat
- Unigene: 595053 Human
see all -
Alternative names
- 60 kDa chaperonin antibody
- 60 kDa heat shock protein, mitochondrial antibody
- CH60_HUMAN antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat stomach tissue labeling Hsp60 with ab216634 at 1/500 dilution, followed by conjugation to the secondary antibody and DAB staining.
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Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat brain tissue labeling Hsp60 with ab216634 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
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Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human lung carcinoma tissue labeling Hsp60 with ab216634 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
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Anti-Hsp60 antibody (ab216634) at 1/300 dilution + mouse liver lysate
Secondary
Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/5000 dilution
Predicted band size: 61 kDa -
Anti-Hsp60 antibody (ab216634) at 1/300 dilution + mouse kidney lysate
Secondary
Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/5000 dilution
Predicted band size: 61 kDa -
Anti-Hsp60 antibody (ab216634) at 1/300 dilution + mouse heart lysate
Secondary
Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/5000 dilution
Predicted band size: 61 kDa -
Anti-Hsp60 antibody (ab216634) at 1/300 dilution + MCF7 lysate
Secondary
Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/5000 dilution
Predicted band size: 61 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab216634 has not yet been referenced specifically in any publications.