• Product name
  • Description
    Rabbit polyclonal to Hsp60
  • Host species
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Cow, Human
    Predicted to work with: Mouse, Rat, Chicken, Pufferfish, Zebrafish, Sea urchin, Chinese hamster
  • Immunogen

    Synthetic peptide within Human Hsp60 aa 1-100. The exact sequence is proprietary.
    Database link: P10809

  • Positive control
    • Hela cells, NIH3T3 cells, Colon carcinoma.



Our Abpromise guarantee covers the use of ab31115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 60 kDa.
IHC-P 1/200.


  • Function
    Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
  • Involvement in disease
    Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
    Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life.
  • Sequence similarities
    Belongs to the chaperonin (HSP60) family.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • 60 kDa chaperonin antibody
    • 60 kDa heat shock protein, mitochondrial antibody
    • CH60_HUMAN antibody
    • Chaperonin 60 antibody
    • Chaperonin, 60-KD antibody
    • CPN60 antibody
    • fa04a05 antibody
    • GROEL antibody
    • heat shock 60kDa protein 1 (chaperonin) antibody
    • Heat shock protein 1 (chaperonin) antibody
    • Heat shock protein 60 antibody
    • Heat shock protein 65 antibody
    • heat shock protein family D (Hsp60) member 1 antibody
    • HLD4 antibody
    • Hsp 60 antibody
    • HSP 65 antibody
    • HSP-60 antibody
    • HSP60 antibody
    • HSP65 antibody
    • HSPD1 antibody
    • HuCHA60 antibody
    • Mitochondrial matrix protein P1 antibody
    • P60 lymphocyte protein antibody
    • short heat shock protein 60 Hsp60s1 antibody
    • SPG13 antibody
    see all


  • All lanes : Anti-Hsp60 antibody (ab31115) at 1/8000 dilution

    Lanes 1 & 5 : Cow normal mammary epithelial cells (nMEC) treated with serum free media
    Lanes 2 & 6 : Cow normal mammary epithelial cells (nMEC) treated with 1.0µM anisomycin
    Lanes 3 & 7 : Cow normal mammary epithelial cells (nMEC) treated with 100ng/ml IGF-1
    Lanes 4 & 8 : Cow normal mammary epithelial cells (nMEC) treated with anisomycin and IGF-1

    Lysates/proteins at 40 µg per lane.

    All lanes : ECL Anti-Rabbit IgG, Horseradish Peroxidase Linked at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 60 kDa
    Observed band size: 60 kDa

    Exposure time: 15 seconds

    Treatment for 30 minutes (lanes 1-4) or 1 hour (lanes 5-8). 12.5% SDS-PAGE. Proteins collected in RIPA Buffer and transferred to PVDF (0.45 um).

    See Abreview

  • Ab31115, at a 1/200 dilution, staining Hsp60 in formalin fixed, paraffin embedded human colon carcinoma tissue by Immunohistochemistry.
  • Anti-Hsp60 antibody (ab31115) at 1/25 dilution + lysate prepared from HeLa cells

    Predicted band size: 60 kDa


This product has been referenced in:
  • Jetzt AE  et al. Toxicity of ricin A chain is reduced in mammalian cells by inhibiting its interaction with the ribosome. Toxicol Appl Pharmacol 310:120-128 (2016). Read more (PubMed: 27639428) »
  • Vale-Costa S  et al. Influenza A virus ribonucleoproteins modulate host recycling by competing with Rab11 effectors. J Cell Sci 129:1697-710 (2016). Read more (PubMed: 26940915) »
See all 5 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (12.5% SDS-PAGE)
Cow Cell lysate - whole cell (Normal mammary epithelial cells (nMEC))
Normal mammary epithelial cells (nMEC)
1.0 uM ANS +/- 100 ng/mL IGF-1
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Sep 27 2013


Sign up