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    hsp60-antibody-ab46798.pdf

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Anti-Hsp60 antibody (ab46798)

  • Datasheet
Reviews (13)Q&A (1)References (100)

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Western blot - Anti-Hsp60 antibody (ab46798)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody (ab46798)
  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp60 antibody (ab46798)
  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp60 antibody (ab46798)
  • Flow Cytometry - Anti-Hsp60 antibody (ab46798)
  • Immunoprecipitation - Anti-Hsp60 antibody (ab46798)
  • Western blot - Anti-Hsp60 antibody (ab46798)
  • Western blot - Anti-Hsp60 antibody (ab46798)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody (ab46798)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody (ab46798)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody (ab46798)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody (ab46798)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody (ab46798)
  • Western blot - Anti-Hsp60 antibody (ab46798)
  • Western blot - Anti-Hsp60 antibody (ab46798)

Key features and details

  • Rabbit polyclonal to Hsp60
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
  • Reacts with: Mouse, Rat, Human, Pig
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Hsp60 antibody [EPR18245-93] (ab190828)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Hsp60 antibody
    See all Hsp60 primary antibodies
  • Description

    Rabbit polyclonal to Hsp60
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Pig
    Predicted to work with: Chicken, Hamster, Cow, Arabidopsis thaliana, Chlamydomonas reinhardtii, Orangutan, Secale cereale, Brassica napus, Heliothis virescens, Syntrophobacter fumaroxidans, Methylacidiphilum infernorum, Thermodesulfovibrio yellowstonii, Pisum sativum
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Heat Shock Proteins
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Signal transduction proteins ELISA kits
    • Isotype/Loading Controls
    • Loading Controls
    • Hsp60

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab6721)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • T-47D whole cell lysate (ab14899)
    • HeLa whole cell lysate (ab150035)
  • Recombinant Protein

    • Recombinant human Hsp60 protein (ab113192)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab46798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (8)
1/20000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
IHC-P (2)
1/100.
ICC/IF (1)
Use a concentration of 5 - 10 µg/ml.
Flow Cyt
1/30.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IP (1)
1/40.
Notes
WB
1/20000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
IHC-P
1/100.
ICC/IF
Use a concentration of 5 - 10 µg/ml.
Flow Cyt
1/30.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IP
1/40.

Target

  • Function

    Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
  • Involvement in disease

    Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
    Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life.
  • Sequence similarities

    Belongs to the chaperonin (HSP60) family.
  • Cellular localization

    Mitochondrion matrix.
  • Target information above from: UniProt accession P10809 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 424059 Chicken
    • Entrez Gene: 511913 Cow
    • Entrez Gene: 101844216 Hamster
    • Entrez Gene: 3329 Human
    • Entrez Gene: 15510 Mouse
    • Entrez Gene: 492279 Pig
    • Entrez Gene: 63868 Rat
    • Omim: 118190 Human
    • SwissProt: Q5ZL72 Chicken
    • SwissProt: P31081 Cow
    • SwissProt: P10809 Human
    • SwissProt: P63038 Mouse
    • SwissProt: P63039 Rat
    • Unigene: 595053 Human
    • Unigene: 727543 Human
    • Unigene: 1777 Mouse
    • Unigene: 102058 Rat
    see all
  • Alternative names

    • 60 kDa chaperonin antibody
    • 60 kDa heat shock protein, mitochondrial antibody
    • CH60_HUMAN antibody
    • Chaperonin 60 antibody
    • Chaperonin, 60-KD antibody
    • CPN60 antibody
    • fa04a05 antibody
    • GROEL antibody
    • heat shock 60kDa protein 1 (chaperonin) antibody
    • Heat shock protein 1 (chaperonin) antibody
    • Heat shock protein 60 antibody
    • Heat shock protein 65 antibody
    • heat shock protein family D (Hsp60) member 1 antibody
    • HLD4 antibody
    • Hsp 60 antibody
    • HSP 65 antibody
    • HSP-60 antibody
    • HSP60 antibody
    • HSP65 antibody
    • HSPD1 antibody
    • HuCHA60 antibody
    • Mitochondrial matrix protein P1 antibody
    • P60 lymphocyte protein antibody
    • short heat shock protein 60 Hsp60s1 antibody
    • SPG13 antibody
    see all

Images

  • Western blot - Anti-Hsp60 antibody (ab46798)
    Western blot - Anti-Hsp60 antibody (ab46798)
    All lanes : Anti-Hsp60 antibody (ab46798) at 1/20000 dilution (purified)

    Lane 1 : Mouse brain tissue lysate at 20 µg
    Lane 2 : Mouse heart tissue lysate at 20 µg
    Lane 3 : Mouse spleen tissue lysate at 20 µg
    Lane 4 : Rat heart tissue lysate at 10 µg

    Secondary
    All lanes : HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 60 kDa
    Observed band size: 60 kDa



    Blocking Buffer: 5% NFDM/TBST

    Dilution Buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody (ab46798)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody (ab46798)

    Immunohistochemical staining of paraffin embedded human lung adenocarcinoma with purified ab46798 at a working dilution of 1 in 100. The secondary antibody used is ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp60 antibody (ab46798)
    Immunocytochemistry/ Immunofluorescence - Anti-Hsp60 antibody (ab46798)

    Immunofluorescence analysis of HeLa cells labeling Hsp60 with ab46798 at a working dilution of 1 in 50 counter-stained with DAPI. The secondary antibody was ab150077 Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 500. Tubulin was stained with mouse anti-tubulin at a dilution of 1/1000 (ab7291) and Alexa Fluor® 594 goat anti-mouse at a dilution of 1/500 (ab150120) . The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in the bottom middle and right hand panels - for the first negative control, purified ab46798 was used at a dilution of 1/200 followed by an Alexa Fluor® 555 goat anti-mouse antibody at a dilution of 1/500 and for the second negative control mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab15007) were used.

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp60 antibody (ab46798)
    Immunocytochemistry/ Immunofluorescence - Anti-Hsp60 antibody (ab46798)

    ab46798 stained in Hela cells. Cells were fixed with 100% methanol (5 min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab46798 at 10 µg/ml and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control) at 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150120 (pseudo-colored red) and ab150081 (colored green) used at 1 µg/ml for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Flow Cytometry - Anti-Hsp60 antibody (ab46798)
    Flow Cytometry - Anti-Hsp60 antibody (ab46798)

    Overlay histogram showing HeLa cells fixed in 2% PFA and stained with purified ab46798 at a dilution of 1 in 150 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 150. Rabbit monoclonal IgG was used as an isotype control (black) and cells without incubation with the antibody were used as a negative control (blue line).

  • Immunoprecipitation - Anti-Hsp60 antibody (ab46798)
    Immunoprecipitation - Anti-Hsp60 antibody (ab46798)

    ab46798 (purified) at 1/40 immunoprecipitating Hsp60 in MCF7 (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-Hsp60 antibody (ab46798)
    Western blot - Anti-Hsp60 antibody (ab46798)
    Anti-Hsp60 antibody (ab46798) at 1/20000 dilution (purified) + T47-D cell lysate at 10 µg

    Secondary
    HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 60 kDa
    Observed band size: 60 kDa



    Blocking Buffer: 5% NFDM/TBST

    Dilution Buffer: 5% NFDM/TBST

  • Western blot - Anti-Hsp60 antibody (ab46798)
    Western blot - Anti-Hsp60 antibody (ab46798)
    All lanes : Anti-Hsp60 antibody (ab46798) at 1/50000 dilution (purified)

    Lane 1 : MCF7 cell lysate
    Lane 2 : SW480 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 60 kDa
    Observed band size: 60 kDa



    Blocking Buffer: 5% NFDM/TBST

    Dilution Buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody (ab46798)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody (ab46798)

    Immunohistochemical staining of paraffin embedded rat kidney with purified ab46798 at a working dilution of 1 in 100. The secondary antibody used is ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody (ab46798)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody (ab46798)

    Immunohistochemical staining of paraffin embedded mouse cardiac muscle with purified ab46798 at a working dilution of 1 in 100. The secondary antibody used is ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody (ab46798)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody (ab46798)

    Immunohistochemical staining of paraffin embedded human cervical carcinoma with purified ab46798 at a working dilution of 1 in 100. The secondary antibody used is ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody (ab46798)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody (ab46798)This image is courtesy of an Abreview submitted by Nicole Schechter Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Unpurified ab46798 staining Hsp60 from human colon tissue by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Cells were formaldehyde fixed prior to blocking in 10% serum for 2 hours at 21°C. The primary antibody was diluted 1/500 and incubated with the sample for 2 hours at 21°C. Alexa fluor® 594 goat polyclonal, diluted 1/5000, was used as the secondary.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody (ab46798)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody (ab46798)

    Immunohistochemical analysis of paraffin-embedded human breast adenocarcinoma using unpurified ab46798 at 1/250 dilution.

  • Western blot - Anti-Hsp60 antibody (ab46798)
    Western blot - Anti-Hsp60 antibody (ab46798)
    Anti-Hsp60 antibody (ab46798) at 1/50000 dilution (purified) + Pig heart tissue lysate at 20 µg

    Secondary
    HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 60 kDa
    Observed band size: 60 kDa



    Blocking Buffer: 5% NFDM/TBST

    Dilution Buffer: 5% NFDM/TBST

  • Western blot - Anti-Hsp60 antibody (ab46798)
    Western blot - Anti-Hsp60 antibody (ab46798)
    Anti-Hsp60 antibody (ab46798) at 1/50000 dilution (unpurified) + T-47D whole cell lysate (ab14899) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution (Goat anti -rabbit HRP)

    Predicted band size: 60 kDa
    Observed band size: 60 kDa



    Secondary antibody - anti-rabbit HRP (ab6721)

Protocols

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (100)

Publishing research using ab46798? Please let us know so that we can cite the reference in this datasheet.

ab46798 has been referenced in 100 publications.

  • Lee Y  et al. General anesthesia activates the mitochondrial unfolded protein response and induces age-dependent, long-lasting changes in mitochondrial function in the developing brain. Neurotoxicology 82:1-8 (2021). PubMed: 33144179
  • Mesquita PHC  et al. Effects of Resistance Training on the Redox Status of Skeletal Muscle in Older Adults. Antioxidants (Basel) 10:N/A (2021). PubMed: 33652958
  • Sato S  et al. Homeostatic p62 levels and inclusion body formation in CHCHD2 knockout mice. Hum Mol Genet 30:443-453 (2021). PubMed: 33631794
  • Sun MY  et al. Cisplatin-Resistant Gastric Cancer Cells Promote the Chemoresistance of Cisplatin-Sensitive Cells via the Exosomal RPS3-Mediated PI3K-Akt-Cofilin-1 Signaling Axis. Front Cell Dev Biol 9:618899 (2021). PubMed: 33644057
  • Baehr A  et al. Agrin Promotes Coordinated Therapeutic Processes Leading to Improved Cardiac Repair in Pigs. Circulation 142:868-881 (2020). PubMed: 32508131
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
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Immunocytochemistry/ Immunofluorescence abreview for Anti-Hsp60 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa FlipIn Cell line)
Permeabilization
Yes - 0.3% Triton X
Specification
HeLa FlipIn Cell line
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Süleyman Bozkurt

Verified customer

Submitted Jan 25 2022

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