Western blot abreview for Anti-Hsp60 antibody

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - other (Hela cells, mitochondrial fraction)
Loading amount
20 µg
Specification
Hela cells, mitochondrial fraction
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C

Other product details

Dilution
1/5000
Incubation time
3 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: PBS tween 0.1% + BSA 3%

Secondary antibody

Name
Non-Abcam antibody was used: anti rabbit iGG sigma, 1h
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/15000

Detection

Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 60 kDa Non-specific: 25, 20 kDa

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Submitted Sep 09 2008

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