Western blot abreview for Anti-Hsp60 antibody

Average
Abreviews
Application
Western blot
Loading amount
48 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Rat Tissue lysate - whole (Bone tissue (Tibia))
Specification
Bone tissue (Tibia)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in TBS-T
Dilution
1/1500

Secondary antibody

Dilution
1/2000

Detection

Detection method
ECL
Bands
Specific: 60 kDa Non-specific: 27, 68 kDa
Exposure
12 minute(s) and 0 second(s)
Positive control
Samples

Mr. Helder Fonseca

Verified customer

Submitted Oct 06 2014

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