Key features and details
- Rabbit polyclonal to Hsp60
- Suitable for: WB, IHC-P, ICC
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Hsp60 antibody
See all Hsp60 primary antibodies
DescriptionRabbit polyclonal to Hsp60
Tested applicationsSuitable for: WB, IHC-P, ICCmore details
Species reactivityReacts with: Mouse, Rat, Human
A synthetic peptide mapping at the middle region of human Hsp60, identical to the related rat and mouse sequence.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab68416 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 60 kDa.|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml.|
|ICC||Use a concentration of 0.5 - 1 µg/ml.|
FunctionImplicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
Involvement in diseaseDefects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life.
Sequence similaritiesBelongs to the chaperonin (HSP60) family.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- 60 kDa chaperonin antibody
- 60 kDa heat shock protein, mitochondrial antibody
- CH60_HUMAN antibody
All lanes : Anti-Hsp60 antibody (ab68416) at 0.5 µg/ml
Lane 1 : Rat brain tissue lysate
Lane 2 : Rat liver tissue lysate
Lane 3 : CEM cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : SMMC cell lysate
Lane 6 : COLO320 cell lysate
Predicted band size: 60 kDa
Immunocytochemistry of SMMC cells using ab68416 at 1 μg/ml
ab68416 at 1µg/ml staining Hsp60 in Human mammary cancer tissue sections by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). The tissue sections underwent antigen retrieval by boiling. A Biotin-conjugated Goat anti-rabbit IgG was used as secondary at 1/200 dilution.
ab68416 has been referenced in 1 publication.
- Deng MY et al. Frontal-subcortical protein expression following prenatal exposure to maternal inflammation. PLoS One 6:e16638 (2011). WB ; Mouse . PubMed: 21347362