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    hsp60-antibody-ab82513.pdf

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Tags & Cell Markers Subcellular Markers Organelles Mitochondria
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Anti-Hsp60 antibody (ab82513)

  • Datasheet
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Abpromise

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Western blot - Anti-Hsp60 antibody (ab82513)

    Key features and details

    • Rabbit polyclonal to Hsp60
    • Suitable for: WB
    • Reacts with: Mouse, Rat, Human
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant human Hsp60 protein (ab113192)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-Hsp60 antibody
      See all Hsp60 primary antibodies
    • Description

      Rabbit polyclonal to Hsp60
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      Native Hsp60 from Heliothis virescens (insect) sperm

    • Positive control

      • HeLa, 3T3 and PC12 Heat Shocked lysates.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      pH: 7.20
      Preservative: 0.09% Sodium azide
      Constituents: PBS, 50% Glycerol
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Tags & Cell Markers
      • Subcellular Markers
      • Organelles
      • Mitochondria
      • Signal Transduction
      • Protein Trafficking
      • Chaperones
      • Heat Shock Proteins
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Signal transduction proteins ELISA kits
      • Isotype/Loading Controls
      • Loading Controls
      • Hsp60

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant human Hsp60 protein (ab113192)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab82513 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    Application Abreviews Notes
    WB
    1/1000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
    Notes
    WB
    1/1000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).

    Target

    • Function

      Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
    • Involvement in disease

      Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
      Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life.
    • Sequence similarities

      Belongs to the chaperonin (HSP60) family.
    • Cellular localization

      Mitochondrion matrix.
    • Target information above from: UniProt accession P10809 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 3329 Human
      • Entrez Gene: 15510 Mouse
      • Entrez Gene: 63868 Rat
      • Omim: 118190 Human
      • SwissProt: P10809 Human
      • SwissProt: P63038 Mouse
      • SwissProt: P63039 Rat
      • Unigene: 595053 Human
      • Unigene: 727543 Human
      • Unigene: 1777 Mouse
      • Unigene: 102058 Rat
      see all
    • Alternative names

      • 60 kDa chaperonin antibody
      • 60 kDa heat shock protein, mitochondrial antibody
      • CH60_HUMAN antibody
      • Chaperonin 60 antibody
      • Chaperonin, 60-KD antibody
      • CPN60 antibody
      • fa04a05 antibody
      • GROEL antibody
      • heat shock 60kDa protein 1 (chaperonin) antibody
      • Heat shock protein 1 (chaperonin) antibody
      • Heat shock protein 60 antibody
      • Heat shock protein 65 antibody
      • heat shock protein family D (Hsp60) member 1 antibody
      • HLD4 antibody
      • Hsp 60 antibody
      • HSP 65 antibody
      • HSP-60 antibody
      • HSP60 antibody
      • HSP65 antibody
      • HSPD1 antibody
      • HuCHA60 antibody
      • Mitochondrial matrix protein P1 antibody
      • P60 lymphocyte protein antibody
      • short heat shock protein 60 Hsp60s1 antibody
      • SPG13 antibody
      see all

    Images

    • Western blot - Anti-Hsp60 antibody (ab82513)
      Western blot - Anti-Hsp60 antibody (ab82513)
      All lanes : Anti-Hsp60 antibody (ab82513) at 1/1000 dilution

      Lane 1 : HeLa (Heat
      Shocked) cell lysate
      Lane 2 : 3T3 (Heat Shocked),
      cell lysate
      Lane 3 : PC12 (Heat Shocked) cell lysate

      Predicted band size: 61 kDa
      Observed band size: 61 kDa

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab82513? Please let us know so that we can cite the reference in this datasheet.

    ab82513 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    Sehr geehrte Damen und Herren! Wir sind auf der Suche nach a.) polyclonal Hsp60 AK biotinyliert b.) mycobacterial Hsp65 AK biotinyliert, geeignet für Elisa. Haben Sie diese Produkte in Ihrem Sortiment? Vielen Dank im vorraus.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 18 2011

    Answer

    Vielen Dank für Ihre Anfrage. In der Tat haben wir einige polyklonalen HSP60- Antikörper in unserem Katalog verfügbar, unter anderem zum Beispiel ab105853: ein Kaninchen-polyklonaler, der gegen das humane Volllängenprotein gerichtet ist und für Western blot, ELISA und Radioimmunoassay (RIA) geeignet ist. Click here (or use the following: https://www.abcam.com/Biotin-Hsp60-antibody-ab105853.html). Des Weiteren haben wir auch einige Antikörper gegen HSP60 anderer Spezies verfügbar: ab81137: Click here (or use the following: https://www.abcam.com/Legionella-pneumophila-hsp60-antibody-18-ab81137.html). ab82513: Click here (or use the following: https://www.abcam.com/Hsp60-antibody-ab82513.html). ab82520: Click here (or use the following: https://www.abcam.com/Hsp60-antibody-ab82520.html). Die folgenden HSP65- Antikörper sind bei uns erhältlich: ab20045: Click here (or use the following: https://www.abcam.com/Mycobacterium-tuberculosis-Hsp-65-antibody-BDI578-ab20045.html). ab20519: Click here (or use the following: https://www.abcam.com/Mycobacterium-tuberculosis-Hsp-65-antibody-BDI577-ab20519.html). Falls die Antikörper nicht Biotin-konjugiert vorliegen, kann ich Ihnen unsere EasyLink-Kits empfehlen, mit denen Sie jedes Protein/jeden Antikörper mit dem Konjugat Ihrer Wahl koppeln können (s. Anhang oder https://www.abcam.com/EasyLink), wie zum Beispiel: ab102864: Click here (or use the following: https://www.abcam.com/EasyLink-Biotin-type-A-Conjugation-Kit-3-x-100g-Biotin-ab102864.html). ab102867: Click here (or use the following: https://www.abcam.com/Biotin-type-B-Conjugation-Kit-ab102867.html). Wir geben alle getesteten Spezies und Anwendungen auf unseren Produktdatenblättern an und aktualisieren diese sobald wir neue Informationen erhalten. Sollten Sie einen Antikörper gegen das HSP60 oder HSP65 einer anderen Spezies benötigen, bin ich Ihnen gerne bei der weiteren Suche behilflich. Falls Sie einen Antikörper in einer bisher ungetesteten Anwendung oder Spezies testen möchten, kann ich Ihnen zur Zeit ein spezielles Angebot über einen 100%igen Abreview-Rabatt anbieten. Bei diesem Angebot bekommen Sie einen Rabatt für eine zukünftige Bestellung, wenn Sie uns ein Abreview mit dem Testresultat zusenden. Der Rabatt würde gegen eine erneute Bestellung eines weiteren primären Antikörpers von uns verrechnet werden. Weitere Information schicke ich Ihnen auf Anfrage gerne zu. Alle unsere Produkte unterliegen unserer Abcam-Garantie: Falls sich herausstellt, dass der Antikörper nicht so funktioniert, wie auf dem Datenblatt beschrieben und Sie sich innerhalb von sechs Monaten mit Ihrem Problem an uns wenden, werden wir Ihnen gerne Vorschläge zur Optimierung Ihres Protokolls oder gegebenenfalls einen Ersatz oder eine Gutschrift schicken. Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

    Read More

    Abcam Scientific Support

    Answered on Nov 18 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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