• Product name
    Anti-Hsp60 antibody [LK-2]
    See all Hsp60 primary antibodies
  • Description
    Mouse monoclonal [LK-2] to Hsp60
  • Host species
  • Tested applications
    Suitable for: IHC-Fr, IHC-P, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Chicken, Guinea pig, Hamster, Cow, Human, Pig, Saccharomyces cerevisiae, Monkey, Escherichia coli
  • Immunogen

    Human Hsp60 produced through recombinant DNA methods in E.coli

  • Positive control
    • HeLa cell lysate



Our Abpromise guarantee covers the use of ab59458 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.25 µg/ml. Predicted molecular weight: 60 kDa.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.



  • Function
    Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
  • Involvement in disease
    Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
    Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life.
  • Sequence similarities
    Belongs to the chaperonin (HSP60) family.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • 60 kDa chaperonin antibody
    • 60 kDa heat shock protein, mitochondrial antibody
    • CH60_HUMAN antibody
    • Chaperonin 60 antibody
    • Chaperonin, 60-KD antibody
    • CPN60 antibody
    • fa04a05 antibody
    • GROEL antibody
    • heat shock 60kDa protein 1 (chaperonin) antibody
    • Heat shock protein 1 (chaperonin) antibody
    • Heat shock protein 60 antibody
    • Heat shock protein 65 antibody
    • heat shock protein family D (Hsp60) member 1 antibody
    • HLD4 antibody
    • Hsp 60 antibody
    • HSP 65 antibody
    • HSP-60 antibody
    • HSP60 antibody
    • HSP65 antibody
    • HSPD1 antibody
    • HuCHA60 antibody
    • Mitochondrial matrix protein P1 antibody
    • P60 lymphocyte protein antibody
    • short heat shock protein 60 Hsp60s1 antibody
    • SPG13 antibody
    see all


  • Anti-Hsp60 antibody [LK-2] (ab59458) at 1/1000 dilution + HeLa cell lysate

    Predicted band size: 60 kDa

  • Lanes 1-3 : Anti-Hsp60 antibody [LK-2] (ab59458) at 1/4000 dilution

    Lanes 1-3 : Saccharomyces cerevisiae whole cell lysate at 6900000 cells
    Lane 4 : Ladder

    Lanes 1-2 : HRP-conjugated goat anti-mouse IgG monoclonal at 1/5000 dilution
    Lane 3 : HRP-conjugated goat anti-mouse IgG monoclonal at 1/2000 dilution

    Performed under reducing conditions.

    Predicted band size: 60 kDa
    Observed band size: 60 kDa

    Exposure time: 25 seconds

    See Abreview

  • ab59464 at 100,000 dilution staining Hsp60 alpha in human colon tissue section by Immunohistochemistry (Bouin's fixed paraffin embedded tissue sections). Tissue underwent heat mediated antigen retrieval in microwave with 2, 5 minutes incubation intervals in citrate buffer. An antibody amplifier™ system was used for staining. A HRP-conjugated anti mouse secondary was used at 1/10 dilution.

  • Paraffin-embedded mouse back skin tissue stained for Hsp60 using ab59458 at 1/100 dilution in immunohistochemical analysis. DAB staining.


This product has been referenced in:
  • Farrugia G  et al. Aspirin impairs acetyl-coenzyme A metabolism in redox-compromised yeast cells. Sci Rep 9:6152 (2019). Read more (PubMed: 30992471) »
  • Bivi N  et al. Transcriptome and proteome analysis of osteocytes treated with nitrogen-containing bisphosphonates. J Proteome Res 8:1131-42 (2009). Read more (PubMed: 19226166) »
See all 2 Publications for this product

Customer reviews and Q&As

Western blot
Loading amount
6.9e+006 cells
Gel Running Conditions
Reduced Denaturing (4-12 % Bis-Tris, MOPS running buffer, semi-dry transfer to nitrocellulose)
Saccharomyces cerevisiae Cell lysate - whole cell (W303alpha)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Submitted Apr 25 2014

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