Anti-Hsp60 antibody [LK1] (ab230630)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Hsp60 antibody [LK1]
See all Hsp60 primary antibodies -
Description
Mouse monoclonal [LK1] to Hsp60 -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Pig, Monkey
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Immunogen
Recombinant full length protein corresponding to Human Hsp60.
Database link: P10809 -
Positive control
- IHC-P: Human spleen and adrenal tissues.
Properties
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Form
Liquid -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
LK1 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab230630 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. -
Involvement in disease
Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life. -
Sequence similarities
Belongs to the chaperonin (HSP60) family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 424059 Chicken
- Entrez Gene: 511913 Cow
- Entrez Gene: 100715481 Guinea pig
- Entrez Gene: 101844216 Hamster
- Entrez Gene: 3329 Human
- Entrez Gene: 15510 Mouse
- Entrez Gene: 492279 Pig
- Entrez Gene: 100349912 Rabbit
see all -
Alternative names
- 60 kDa chaperonin antibody
- 60 kDa heat shock protein, mitochondrial antibody
- CH60_HUMAN antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK1] (ab230630)](https://www.abcam.com/ps/products/230/ab230630/Images/ab230630-298777-ab2306301.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK1] (ab230630)Formalin-fixed, paraffin-embedded human spleen tissue stained for Hsp60 using ab230630 at 10 µg/ml in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK1] (ab230630)](https://www.abcam.com/ps/products/230/ab230630/Images/ab230630-298776-ab2306302.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK1] (ab230630)Formalin-fixed, paraffin-embedded human adrenal tissue stained for Hsp60 using ab230630 at 10 µg/ml in immunohistochemical analysis.
Datasheets and documents
References
ab230630 has not yet been referenced specifically in any publications.
