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    hsp60-antibody-lk1-ab230630.pdf

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Tags & Cell Markers Subcellular Markers Organelles Mitochondria
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Anti-Hsp60 antibody [LK1] (ab230630)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK1] (ab230630)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK1] (ab230630)

Key features and details

  • Mouse monoclonal [LK1] to Hsp60
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG1

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Protein
Product image
Recombinant human Hsp60 protein (ab113192)
Secondary
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Goat Anti-Mouse IgG H&L (HRP) (ab205719)

View more associated products

Overview

  • Product name

    Anti-Hsp60 antibody [LK1]
    See all Hsp60 primary antibodies
  • Description

    Mouse monoclonal [LK1] to Hsp60
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Pig, Monkey
  • Immunogen

    Recombinant full length protein corresponding to Human Hsp60.
    Database link: P10809

  • Positive control

    • IHC-P: Human spleen and adrenal tissues.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from TCS.
  • Clonality

    Monoclonal
  • Clone number

    LK1
  • Isotype

    IgG1
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Heat Shock Proteins
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Signal transduction proteins ELISA kits

Associated products

  • Alternative Versions

    • Anti-Hsp60 antibody [LK1] - BSA and Azide free (ab212454)
    • Anti-Hsp60 antibody [LK1] (ab233878)
    • Anti-Hsp60 antibody [LK1] - BSA and Azide free (ab269783)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Recombinant Protein

    • Recombinant human Hsp60 protein (ab113192)
  • Related Products

    • Recombinant Human Hsp60 protein (ab113177)
    • Recombinant human Hsp60 protein (ab113192)
    • Recombinant Human Hsp60 protein (ab56277)

Applications

Our Abpromise guarantee covers the use of ab230630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
  • Involvement in disease

    Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
    Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life.
  • Sequence similarities

    Belongs to the chaperonin (HSP60) family.
  • Cellular localization

    Mitochondrion matrix.
  • Target information above from: UniProt accession P10809 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 424059 Chicken
    • Entrez Gene: 511913 Cow
    • Entrez Gene: 100715481 Guinea pig
    • Entrez Gene: 101844216 Hamster
    • Entrez Gene: 3329 Human
    • Entrez Gene: 15510 Mouse
    • Entrez Gene: 492279 Pig
    • Entrez Gene: 100349912 Rabbit
    • Entrez Gene: 63868 Rat
    • Entrez Gene: 101122254 Sheep
    • Omim: 118190 Human
    • SwissProt: Q5ZL72 Chicken
    • SwissProt: P31081 Cow
    • SwissProt: P10809 Human
    • SwissProt: P63038 Mouse
    • SwissProt: P63039 Rat
    • Unigene: 595053 Human
    • Unigene: 727543 Human
    • Unigene: 1777 Mouse
    • Unigene: 102058 Rat
    see all
  • Alternative names

    • 60 kDa chaperonin antibody
    • 60 kDa heat shock protein, mitochondrial antibody
    • CH60_HUMAN antibody
    • Chaperonin 60 antibody
    • Chaperonin, 60-KD antibody
    • CPN60 antibody
    • fa04a05 antibody
    • GROEL antibody
    • heat shock 60kDa protein 1 (chaperonin) antibody
    • Heat shock protein 1 (chaperonin) antibody
    • Heat shock protein 60 antibody
    • Heat shock protein 65 antibody
    • heat shock protein family D (Hsp60) member 1 antibody
    • HLD4 antibody
    • Hsp 60 antibody
    • HSP 65 antibody
    • HSP-60 antibody
    • HSP60 antibody
    • HSP65 antibody
    • HSPD1 antibody
    • HuCHA60 antibody
    • Mitochondrial matrix protein P1 antibody
    • P60 lymphocyte protein antibody
    • short heat shock protein 60 Hsp60s1 antibody
    • SPG13 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK1] (ab230630)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK1] (ab230630)

    Formalin-fixed, paraffin-embedded human spleen tissue stained for Hsp60 using ab230630 at 10 µg/ml in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK1] (ab230630)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK1] (ab230630)

    Formalin-fixed, paraffin-embedded human adrenal tissue stained for Hsp60 using ab230630 at 10 µg/ml in immunohistochemical analysis.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab230630? Please let us know so that we can cite the reference in this datasheet.

    ab230630 has not yet been referenced specifically in any publications.

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