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    hsp70-antibody-ab137680.pdf

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Signal Transduction Protein Trafficking Chaperones Heat Shock Proteins
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Anti-Hsp70 antibody (ab137680)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (2)

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Western blot - Anti-Hsp70 antibody (ab137680)
  • Western blot - Anti-Hsp70 antibody (ab137680)
  • Western blot - Anti-Hsp70 antibody (ab137680)
  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp70 antibody (ab137680)

Key features and details

  • Rabbit polyclonal to Hsp70
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Protein
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Recombinant human Hsp70 protein (Active) (ab78434)

View more associated products

Overview

  • Product name

    Anti-Hsp70 antibody
    See all Hsp70 primary antibodies
  • Description

    Rabbit polyclonal to Hsp70
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment, corresponding to amino acids 308-641 of Human Hsp70(NP_005336)

  • Positive control

    • Recombinant human Hsp70 protein (ab92415) can be used as a positive control in WB. 293T, A431, H1299, HeLaS3, Hela, HepG2, MOLT4, Raji Mouse and Rat brain whole cell lysates; HeLa cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Heat Shock Proteins
    • Cancer
    • Tumor biomarkers
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • Raji whole cell lysate (ab30124)
    • Mouse brain tissue lysate - total protein (ab30151)
    • Recombinant human Hsp70 protein (ab78427)
    • Recombinant human Hsp70 protein (ab78433)
    • Recombinant human Hsp70 protein (Active) (ab78434)
    • A431 whole cell lysate (ab7909)
    • MOLT4 whole cell lysate (ab7912)
    • Recombinant human Hsp70 protein (ab80031)
    • Recombinant human Hsp70 protein (ab92415)
  • Recombinant Protein

    • Recombinant human Hsp70 protein (Active) (ab78434)

Applications

Our Abpromise guarantee covers the use of ab137680 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 70 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Relevance

    Function: In cooperation with other chaperones, the Hsp70 family stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Tissue specificity: HSPA1B is testis-specific.
  • Cellular localization

    Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Database links

    • Entrez Gene: 281825 Cow
    • Entrez Gene: 15511 Human
    • Entrez Gene: 3303 Human
    • Entrez Gene: 193740 Mouse
    • Entrez Gene: 3304 Mouse
    • Entrez Gene: 24472 Rat
    • Entrez Gene: 294254 Rat
    • Omim: 140550 Human
    • Omim: 603012 Human
    • SwissProt: Q27975 Cow
    • SwissProt: P0DMV8 Human
    • SwissProt: P0DMV9 Human
    • SwissProt: P17879 Mouse
    • SwissProt: Q61696 Mouse
    • SwissProt: P0DMW0 Rat
    • SwissProt: P0DMW1 Rat
    see all
  • Alternative names

    • DnaK type molecular chaperone HSP70 1 antibody
    • Epididymis secretory protein Li 103 antibody
    • FLJ54303 antibody
    • FLJ54370 antibody
    • FLJ54392 antibody
    • FLJ54408 antibody
    • FLJ75127 antibody
    • Heat shock 70 kDa protein 1 antibody
    • Heat shock 70 kDa protein 1/2 antibody
    • Heat shock 70 kDa protein 1A antibody
    • Heat shock 70 kDa protein 1A/1B antibody
    • Heat shock 70kDa protein 1B antibody
    • Heat shock induced protein antibody
    • HEL S 103 antibody
    • HSP70 1 antibody
    • HSP70 1A antibody
    • HSP70 1B antibody
    • HSP70 2 antibody
    • HSP70-1 antibody
    • HSP70-1/HSP70-2 antibody
    • HSP70-1A antibody
    • HSP70-2 antibody
    • HSP70.1 antibody
    • HSP70.1/HSP70.2 antibody
    • HSP71_HUMAN antibody
    • HSP72 antibody
    • HSPA1 antibody
    • HSPA1A antibody
    • HSPA1B antibody
    see all

Images

  • Western blot - Anti-Hsp70 antibody (ab137680)
    Western blot - Anti-Hsp70 antibody (ab137680)
    All lanes : Anti-Hsp70 antibody (ab137680) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : H1299 whole cell lysate
    Lane 4 : HeLa S3 whole cell lysate

    Lane 5 : HepG2 whole cell lysate
    Lane 6 : MOLT4 whole cell lysate
    Lane 7 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 70 kDa



    7.5% SDS PAGE
  • Western blot - Anti-Hsp70 antibody (ab137680)
    Western blot - Anti-Hsp70 antibody (ab137680)
    Anti-Hsp70 antibody (ab137680) at 1/2500 dilution + Rat brain whole cell lysate at 40 µg

    Predicted band size: 70 kDa

  • Western blot - Anti-Hsp70 antibody (ab137680)
    Western blot - Anti-Hsp70 antibody (ab137680)
    Anti-Hsp70 antibody (ab137680) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 70 kDa



    7.5% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp70 antibody (ab137680)
    Immunocytochemistry/ Immunofluorescence - Anti-Hsp70 antibody (ab137680)
    Immunofluorescence analysis of methanol-fixed HeLa cells labelling Hsp70 with ab137680 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (2)

    Publishing research using ab137680? Please let us know so that we can cite the reference in this datasheet.

    ab137680 has been referenced in 2 publications.

    • Chittoor-Vinod VG  et al. HSP90 Inhibitor, NVP-AUY922, Improves Myelination in Vitro and Supports the Maintenance of Myelinated Axons in Neuropathic Mice. ACS Chem Neurosci 10:2890-2902 (2019). PubMed: 31017387
    • Lu J  et al. Hypothermic properties of dexmedetomidine provide neuroprotection in rats following cerebral ischemia-reperfusion injury. Exp Ther Med 18:817-825 (2019). PubMed: 31258715

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Hsp70 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Pancreatic cancer)
    Permeabilization
    Yes - 0.1% Triton X-100
    Specification
    Pancreatic cancer
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 30 2020

    Flow Cytometry abreview for Anti-Hsp70 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (Glioblastoma)
    Permeabilization
    Yes - 0.1% PBS-Tween
    Gating Strategy
    No gate
    Specification
    Glioblastoma
    Fixation
    Formaldehyde
    Read More

    Ahmet Kaynak

    Verified customer

    Submitted Sep 30 2020

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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