Anti-Hsp70 antibody (ab137680)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-Hsp70 antibody
See all Hsp70 primary antibodies -
Description
Rabbit polyclonal to Hsp70 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow
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Immunogen
Recombinant fragment, corresponding to amino acids 308-641 of Human Hsp70(NP_005336)
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Positive control
- Recombinant human Hsp70 protein (ab92415) can be used as a positive control in WB. 293T, A431, H1299, HeLaS3, Hela, HepG2, MOLT4, Raji Mouse and Rat brain whole cell lysates; HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
- HeLa whole cell lysate (ab29545)
- Raji whole cell lysate (ab30124)
- Mouse brain tissue lysate - total protein (ab30151)
- Recombinant human Hsp70 protein (ab78427)
- Recombinant human Hsp70 protein (ab78433)
- Recombinant human Hsp70 protein (Active) (ab78434)
- HepG2 whole cell lysate (ab7900)
- A431 whole cell lysate (ab7909)
- MOLT4 whole cell lysate (ab7912)
- Recombinant human Hsp70 protein (ab80031)
- Recombinant human Hsp70 protein (ab92415)
- 293T whole cell lysate (ab95494)
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab137680 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 70 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Relevance
Function: In cooperation with other chaperones, the Hsp70 family stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Tissue specificity: HSPA1B is testis-specific. -
Cellular localization
Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. -
Database links
- Entrez Gene: 281825 Cow
- Entrez Gene: 15511 Human
- Entrez Gene: 3303 Human
- Entrez Gene: 193740 Mouse
- Entrez Gene: 3304 Mouse
- Entrez Gene: 24472 Rat
- Entrez Gene: 294254 Rat
- Omim: 140550 Human
see all -
Alternative names
- DnaK type molecular chaperone HSP70 1 antibody
- Epididymis secretory protein Li 103 antibody
- FLJ54303 antibody
see all
Images
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Western blot - Anti-Hsp70 antibody (ab137680)All lanes : Anti-Hsp70 antibody (ab137680) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lane 4 : HeLa S3 whole cell lysate
Lane 5 : HepG2 whole cell lysate
Lane 6 : MOLT4 whole cell lysate
Lane 7 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 70 kDa
7.5% SDS PAGE -
Western blot - Anti-Hsp70 antibody (ab137680)Anti-Hsp70 antibody (ab137680) at 1/2500 dilution + Rat brain whole cell lysate at 40 µg
Predicted band size: 70 kDa -
Western blot - Anti-Hsp70 antibody (ab137680)Anti-Hsp70 antibody (ab137680) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 70 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Hsp70 antibody (ab137680)Immunofluorescence analysis of methanol-fixed HeLa cells labelling Hsp70 with ab137680 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
References
This product has been referenced in:
- Chittoor-Vinod VG et al. HSP90 Inhibitor, NVP-AUY922, Improves Myelination in Vitro and Supports the Maintenance of Myelinated Axons in Neuropathic Mice. ACS Chem Neurosci 10:2890-2902 (2019). Read more (PubMed: 31017387) »
- Lu J et al. Hypothermic properties of dexmedetomidine provide neuroprotection in rats following cerebral ischemia-reperfusion injury. Exp Ther Med 18:817-825 (2019). Read more (PubMed: 31258715) »
