Product nameAnti-Hsp70 antibody
See all Hsp70 primary antibodies
DescriptionRabbit polyclonal to Hsp70
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Mouse, Rat, Dog, Human, Caenorhabditis elegans, Zebrafish
Predicted to work with: Sheep, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Pig, African green monkey
- Liver lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab66262 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1.25 µg/ml. Predicted molecular weight: 70 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
Titre using peptide based assay 1/312500.
RelevanceFunction: In cooperation with other chaperones, the Hsp70 family stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Tissue specificity: HSPA1B is testis-specific.
Cellular localizationCytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
- DnaK type molecular chaperone HSP70 1 antibody
- Epididymis secretory protein Li 103 antibody
- FLJ54303 antibody
Anti-Hsp70 antibody (ab66262) at 1.25 µg/ml + Human fetal liver lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 70 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Antibody diluted in 5% skim milk / PBS buffer. Gel concentration 12%.
ab66262 has not yet been referenced specifically in any publications.