Key features and details
- Rabbit polyclonal to Hsp70
- Suitable for: WB
- Reacts with: Mouse, Rat, Sheep, Hamster, Cow, Dog, Human, Pig, Monkey
- Isotype: IgG
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab69413 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).|
RelevanceFunction: In cooperation with other chaperones, the Hsp70 family stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Tissue specificity: HSPA1B is testis-specific.
Cellular localizationCytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
- DnaK type molecular chaperone HSP70 1 antibody
- Epididymis secretory protein Li 103 antibody
- FLJ54303 antibody
All lanes : Anti-Hsp70 antibody (ab69413) at 1/1000 dilution
Lane 1 : Hsp70 recombinant human protein
Lane 2 : Hsc70 recombinant bovine protein
Lane 3 : Cell lysates prepared from heat shock treated Hela cells
Lane 4 : Cell lysates prepared from heat shock treated PC-12 cells
Lane 5 : Cell lysates prepared from heat shock treated Vero cells
Lane 6 : Cell lysates prepared from heat shock treated CHO-K1 cells
Predicted band size: 70 kDa
ab69413 has not yet been referenced specifically in any publications.