Key features and details
- Chicken polyclonal to Hsp70
- Suitable for: WB, ELISA, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgY
Storage instructionsShipped at room temperature. Store at -20°C.
Storage bufferpH: 7.40
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab74440 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000. Predicted molecular weight: 70 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceFunction: In cooperation with other chaperones, the Hsp70 family stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Tissue specificity: HSPA1B is testis-specific.
Cellular localizationCytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
- DnaK type molecular chaperone HSP70 1 antibody
- Epididymis secretory protein Li 103 antibody
- FLJ54303 antibody
Immunocytochemistry/Immunofluorescence analysis using ab74440 at 1/120 for 12 hours at 4°C in Heat Shocked HeLa Cells. Fixed by 2% Formaldehyde for 20 min at RT. Secondary Antibody: R-PE Goat Anti-Chicken (yellow) at 1/200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1/40000 for 2 hours at RT. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp70 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
Anti-Hsp70 antibody (ab74440) at 1/1000 dilution + Human HeLa cell lysates
Donkey Anti-Rabbit IgG: HRP
Predicted band size: 70 kDa
Immunocytochemistry/Immunofluorescence analysis using ab74440 at 1/120 for 12 hours at 4°C in Heat Shocked HeLa Cells. Fied by 2% Formaldehyde for 20 min at RT. Secondary Antibody: FITC Goat Anti-Chicken (green) at 1/200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1/40000 for 2 hours at RT. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp70 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab74440 has not yet been referenced specifically in any publications.