Overview

  • Product name

  • Description

    Chicken polyclonal to Hsp70
  • Host species

    Chicken
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Human full length hsp70

Properties

Applications

Our Abpromise guarantee covers the use of ab74440 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    ELISA: Use at an assay dependent dilution.
    WB: 1/1000. Predicted molecular weight: 70 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      Function: In cooperation with other chaperones, the Hsp70 family stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Tissue specificity: HSPA1B is testis-specific.
    • Cellular localization

      Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
    • Database links

    • Alternative names

      • DnaK type molecular chaperone HSP70 1 antibody
      • Epididymis secretory protein Li 103 antibody
      • FLJ54303 antibody
      • FLJ54370 antibody
      • FLJ54392 antibody
      • FLJ54408 antibody
      • FLJ75127 antibody
      • Heat shock 70 kDa protein 1 antibody
      • Heat shock 70 kDa protein 1/2 antibody
      • Heat shock 70 kDa protein 1A antibody
      • Heat shock 70 kDa protein 1A/1B antibody
      • Heat shock 70kDa protein 1B antibody
      • Heat shock induced protein antibody
      • HEL S 103 antibody
      • HSP70 1 antibody
      • HSP70 1A antibody
      • HSP70 1B antibody
      • HSP70 2 antibody
      • HSP70-1 antibody
      • HSP70-1/HSP70-2 antibody
      • HSP70-1A antibody
      • HSP70-2 antibody
      • HSP70.1 antibody
      • HSP70.1/HSP70.2 antibody
      • HSP71_HUMAN antibody
      • HSP72 antibody
      • HSPA1 antibody
      • HSPA1A antibody
      • HSPA1B antibody
      see all

    Images

    • Immunocytochemistry/Immunofluorescence analysis using ab74440 at 1/120 for 12 hours at 4°C in Heat Shocked HeLa Cells. Fixed by 2% Formaldehyde for 20 min at RT. Secondary Antibody: R-PE Goat Anti-Chicken (yellow) at 1/200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1/40000 for 2 hours at RT. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp70 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

    • Anti-Hsp70 antibody (ab74440) at 1/1000 dilution + Human HeLa cell lysates

      Secondary
      Donkey Anti-Rabbit IgG: HRP

      Predicted band size: 70 kDa

    • Immunocytochemistry/Immunofluorescence analysis using ab74440 at 1/120 for 12 hours at 4°C in Heat Shocked HeLa Cells. Fied by 2% Formaldehyde for 20 min at RT. Secondary Antibody: FITC Goat Anti-Chicken (green) at 1/200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1/40000 for 2 hours at RT. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp70 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

    References

    ab74440 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab74440.
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