Anti-Hsp70 antibody (ab79852)
Key features and details
- Rabbit polyclonal to Hsp70
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: n/a
Overview
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Product name
Anti-Hsp70 antibody
See all Hsp70 primary antibodies -
Description
Rabbit polyclonal to Hsp70 -
Host species
Rabbit -
Specificity
Detects a 70kDa protein corresponding to the molecular mass of inducible hsp70. May cross-react with Hsc70 at lower dilutions. -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Horse, Chicken, Cow, Dog, Pig, Xenopus laevis, Macaque monkey, African green monkey, Chinese hamster, Orangutan, Bos mutus grunniens, Saguinus oedipus -
Immunogen
Recombinant full length protein corresponding to Human Hsp70 aa 1 to the C-terminus.
Database link: P0DMV8/P0DMV9 -
Positive control
- IHC-P: mouse Inflamed colon tissue; human colon carcinoma.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab79852 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF | (2) |
1/100.
|
WB | (1) |
1/10000. Detects a band of approximately 70 kDa.
|
IHC-P |
1/100 - 1/1000.
|
Notes |
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ICC/IF
1/100. |
WB
1/10000. Detects a band of approximately 70 kDa. |
IHC-P
1/100 - 1/1000. |
Target
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Relevance
Function: In cooperation with other chaperones, the Hsp70 family stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Tissue specificity: HSPA1B is testis-specific. -
Cellular localization
Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. -
Database links
- Entrez Gene: 281825 Cow
- Entrez Gene: 403612 Dog
- Entrez Gene: 15511 Human
- Entrez Gene: 3303 Human
- Entrez Gene: 193740 Mouse
- Entrez Gene: 3304 Mouse
- Entrez Gene: 396906 Pig
- Entrez Gene: 24472 Rat
see all -
Alternative names
- DnaK type molecular chaperone HSP70 1 antibody
- Epididymis secretory protein Li 103 antibody
- FLJ54303 antibody
see all
Images
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All lanes : Anti-Hsp70 antibody (ab79852) at 1/25000 dilution
Lane 1 : Cell lysates prepared from mouse Pam212 cells
Lane 2 : Molecular weight marker -
Immunohistochemistry of human colon carcinoma staining Hsp70 using ab79852 at 1:50000. A Biotin conjugated Goat Anti-Rabbit antibody at 1:2000 was used as a secondary antibody and Methyl Green at 200uL was used to counter stain.
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Immunohistochemical analysis of paraffin-embedded formalin fixed (4%) mouse Inflamed colon tissue labelling Hsp70 with ab79852 at 1/1000 dilution for 12 hours at 4°C, followed by secondary antibody Goat Anti-Rabbit (Biotin) at 1/2000 dilution for 1 hour at RT. Counter stained with Methyl Green at 200uL for 2 min at RT.
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Immunocytochemistry/Immunofluorescence analysis of heat shocked HeLa cells staining Hsp70 using ab79852 at a 1:100 dilution. The secondary antibody was a FITC conjugated Goat Anti-Rabbit (green) at a 1:200 dilution. Counterstain: DAPI (blue) nuclear stain at 1:40000. A) DAPI (blue) nuclear stain. (B) Anti-Hsp70 Antibody. (C) Composite.
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ab79852 staining Hsp70 in mouse backskin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
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All lanes : Anti-Hsp70 antibody (ab79852) at 1/25000 dilution
Lane 1 : Molecular weight marker
Lane 2 : Cell lysates prepared from human A431 cells
Lane 3 : Cell lysates prepared from human A549 cells
Lane 4 : Cell lysates prepared from human HCT116 cells
Lane 5 : Cell lysates prepared from Hela cells
Lane 6 : Cell lysates prepared from HEK293 cells
Lane 7 : Cell lysates prepared from HepG2 cells
Lane 8 : Cell lysates prepared from HL-60 cells
Lane 9 : Cell lysates prepared from HUVEC cells
Lane 10 : Cell lysates prepared from Jurkat cells
Lane 11 : Cell lysates prepared from MCF7 cells
Lane 12 : Cell lysates prepared from PC3 cells
Lane 13 : Cell lysates prepared from T98G cells
Lane 14 : Tissue lysates prepared from Rat brain
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (52)
ab79852 has been referenced in 52 publications.
- Panachan J et al. Extracellular Vesicle-Based Method for Detecting MYCN Amplification Status of Pediatric Neuroblastoma. Cancers (Basel) 14:N/A (2022). PubMed: 35681607
- Song C et al. HSP70 alleviates sepsis-induced cardiomyopathy by attenuating mitochondrial dysfunction-initiated NLRP3 inflammasome-mediated pyroptosis in cardiomyocytes. Burns Trauma 10:tkac043 (2022). PubMed: 36439706
- Uchida A et al. SOX17-positive rete testis epithelium is required for Sertoli valve formation and normal spermiogenesis in the male mouse. Nat Commun 13:7860 (2022). PubMed: 36543770
- Yang C et al. Exosome miR-134-5p restrains breast cancer progression via regulating PI3K/AKT pathway by targeting ARHGAP1. J Obstet Gynaecol Res 47:4037-4048 (2021). PubMed: 34378285
- Parry HA et al. Short and long-term effect of reproduction on mitochondrial dynamics and autophagy in rats. Heliyon 7:e08070 (2021). PubMed: 34622072