Overview

  • Product name
  • Description
    Rabbit polyclonal to Hsp70
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig, Zebrafish
  • Immunogen

    Recombinant fragment, corresponding to a sequence within amino acids 308-579 of Human Hsp70 (NP_005336).

  • Positive control
    • A431 and Raji whole cell lysates; A431 cells; 293T, H1299, HeLa, HepG2, and MOLT4 cell lines

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 1.21% Tris, 0.75% Glycine
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 70 kDa.
ICC/IF 1/100 - 1/200.

Target

Images

  • All lanes : Anti-Hsp70 antibody (ab97699) at 1/2000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : Hela
    whole cell lysate
    Lane 3 : Hep G2 Whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 70 kDa



    7.5% SDS-PAGE.
  • ab97699, at 1/200 dilution, staining Hsp70 in paraformaldehyde-fixed A431 cells by Immunofluorescence. Lower image is merged with DNA probe.

References

ab97699 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab97699.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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