Anti-Hsp70 antibody [BB70] (ab53496)
Key features and details
- Mouse monoclonal [BB70] to Hsp70
- Suitable for: IHC-P, ICC/IF, Flow Cyt, WB
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG2a
Overview
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Product name
Anti-Hsp70 antibody [BB70]
See all Hsp70 primary antibodies -
Description
Mouse monoclonal [BB70] to Hsp70 -
Host species
Mouse -
Specificity
Detects ~72 (Hsp) and ~73kDa (Hsc).
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Tested applications
Suitable for: IHC-P, ICC/IF, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human -
Immunogen
Other Immunogen Type corresponding to Chicken Hsp70.
Database link: P08106 -
Positive control
- WB: Hela cell lysate; Bovine MDBK cell lysate. IHC-P: Mouse and human colon cancer tissues. Flow Cyt: Jurkat cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
BB70 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53496 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200.
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ICC/IF |
1/200.
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Flow Cyt |
Use 0.5µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000. Predicted molecular weight: 70 kDa.
Detects 72kDa and 73kDa proteins corresponding to the molecular mass of inducible hsp and hsc70 on SDS PAGE immunoblots. |
Notes |
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IHC-P
1/200. |
ICC/IF
1/200. |
Flow Cyt
Use 0.5µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
WB
1/1000. Predicted molecular weight: 70 kDa. Detects 72kDa and 73kDa proteins corresponding to the molecular mass of inducible hsp and hsc70 on SDS PAGE immunoblots. |
Target
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Relevance
Function: In cooperation with other chaperones, the Hsp70 family stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Tissue specificity: HSPA1B is testis-specific. -
Cellular localization
Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. -
Database links
- Entrez Gene: 281825 Cow
- Entrez Gene: 15511 Human
- Entrez Gene: 3303 Human
- Entrez Gene: 193740 Mouse
- Entrez Gene: 3304 Mouse
- Entrez Gene: 24472 Rat
- Entrez Gene: 294254 Rat
- Omim: 140550 Human
see all -
Alternative names
- DnaK type molecular chaperone HSP70 1 antibody
- Epididymis secretory protein Li 103 antibody
- FLJ54303 antibody
see all
Images
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ab53496 at 100,000 dilution staining Hsp70 + Hsc70 in mouse colon cancer tissue section by immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). A antibody amplifier™ system was used for staining. A HRP-conjugated secondary antibody was used at 1/10 dilution.
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Anti-Hsp70 antibody [BB70] (ab53496) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Predicted band size: 70 kDa -
Ab53496, at a dilution of 1/200, staining Hsp70 + Hsc70 in liver smears of rat hepatocytes by Immunohistochemistry Formalin fixed Parafin embedded tissue.
1. Control
2. Two hours after exposure tp hyperthermic stress
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ab53496 at 100,000 dilution staining Hsp70 + Hsc70 in human colon cancer tissue section by immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). A antibody amplifier™ system was used for staining.
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Anti-Hsp70 antibody [BB70] (ab53496) at 1/1000 dilution + Bovine MDBK cells
Predicted band size: 70 kDa -
Ab53496, at a 1/200 dilution, staining Hsp70 + Hsc70 in nuclear smears of rat hepatocytes by immunocytochemistry.
1. Control
2.Five hours after exposure to hyperthermic stress
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ab53496 staining Hsp70 in LLC-PK1 cells expressing Hsp70 under cold block conditions, by Immunocytochemistry/ Immunofluorescence.
Cells were fixed with 4% paraformaldehyde, permeabilized with 0.01% Triton X-100 in PBS for 4 minutes and blocked with 1% BSA/PBS for 20 minutes. Samples were incubated with primary antibody overnight at 4°C. -
Overlay histogram showing Jurkat cells stained with ab53496 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53496, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab53496 has been referenced in 7 publications.
- Zhou C et al. Gut Microbiota and Serum Metabolite Potential Interactions in Growing Layer Hens Exposed to High-Ambient Temperature. Front Nutr 9:877975 (2022). PubMed: 35571932
- Sugito S et al. Immunohistochemical Expression of AQP2 and HSP70 in Broiler Kidney Tissue Treated with Salix tetrasperma Roxb. Extract under Heat Exposure. ScientificWorldJournal 2021:8711286 (2021). PubMed: 34707467
- Kim JW et al. Leptin Modulates the Metastasis of Canine Inflammatory Mammary Adenocarcinoma Cells Through Downregulation of Lysosomal Protective Protein Cathepsin A (CTSA). Int J Mol Sci 21:N/A (2020). PubMed: 33255835
- Ha J et al. Hydrogen sulfide inhibits Kir2 and Kir3 channels by decreasing sensitivity to the phospholipid phosphatidylinositol 4,5-bisphosphate (PIP2). J Biol Chem 293:3546-3561 (2018). PubMed: 29317494
- Tang S et al. Rosemary Reduces Heat Stress by Inducing CRYAB and HSP70 Expression in Broiler Chickens. Oxid Med Cell Longev 2018:7014126 (2018). PubMed: 30425783
- Xiao B et al. Endothelial cell-derived exosomes protect SH-SY5Y nerve cells against ischemia/reperfusion injury. Int J Mol Med 40:1201-1209 (2017). PubMed: 28849073
- Rice WL et al. Differential, phosphorylation dependent trafficking of AQP2 in LLC-PK1 cells. PLoS One 7:e32843 (2012). ICC/IF ; Human . PubMed: 22403603