Overview

  • Product name

    Anti-Hsp70 antibody [BB70]
    See all Hsp70 primary antibodies
  • Description

    Mouse monoclonal [BB70] to Hsp70
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cyt, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Xenopus laevis, Carp, Drosophila melanogaster, Schizosaccharomyces pombe, Rainbow trout
  • Immunogen

    Chicken Hsp70/Hsp90 complex

  • Positive control

    • WB: Hela cell lysate; Bovine MDBK cell lysate. IHC-P: Mouse and human colon cancer tissues. Flow Cyt: Jurkat cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab53496 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ICC/IF 1/200.
Flow Cyt Use 0.5µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 70 kDa. Detects 72kDa and 73kDa proteins corresponding to the molecular mass of inducible hsp and hsc70 on SDS PAGE immunoblots.
IP Use at an assay dependent concentration.

Target

  • Relevance

    Function: In cooperation with other chaperones, the Hsp70 family stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Tissue specificity: HSPA1B is testis-specific.
  • Cellular localization

    Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Database links

  • Alternative names

    • DnaK type molecular chaperone HSP70 1 antibody
    • Epididymis secretory protein Li 103 antibody
    • FLJ54303 antibody
    • FLJ54370 antibody
    • FLJ54392 antibody
    • FLJ54408 antibody
    • FLJ75127 antibody
    • Heat shock 70 kDa protein 1 antibody
    • Heat shock 70 kDa protein 1/2 antibody
    • Heat shock 70 kDa protein 1A antibody
    • Heat shock 70 kDa protein 1A/1B antibody
    • Heat shock 70kDa protein 1B antibody
    • Heat shock induced protein antibody
    • HEL S 103 antibody
    • HSP70 1 antibody
    • HSP70 1A antibody
    • HSP70 1B antibody
    • HSP70 2 antibody
    • HSP70-1 antibody
    • HSP70-1/HSP70-2 antibody
    • HSP70-1A antibody
    • HSP70-2 antibody
    • HSP70.1 antibody
    • HSP70.1/HSP70.2 antibody
    • HSP71_HUMAN antibody
    • HSP72 antibody
    • HSPA1 antibody
    • HSPA1A antibody
    • HSPA1B antibody
    see all

Images

  • ab53496 at 100,000 dilution staining Hsp70 + Hsc70 in mouse colon cancer tissue section by immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). A antibody amplifier™ system was used for staining. A HRP-conjugated secondary antibody was used at 1/10 dilution. 

  • Anti-Hsp70 antibody [BB70] (ab53496) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

    Predicted band size: 70 kDa

  • Ab53496, at a dilution of 1/200, staining Hsp70 + Hsc70 in liver smears of rat hepatocytes by Immunohistochemistry Formalin fixed Parafin embedded tissue.

    1. Control

    2. Two hours after exposure tp hyperthermic stress   

  • ab53496 at 100,000 dilution staining Hsp70 + Hsc70 in human colon cancer tissue section by immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). A antibody amplifier™ system was used for staining.

  • Anti-Hsp70 antibody [BB70] (ab53496) at 1/1000 dilution + Bovine MDBK cells

    Predicted band size: 70 kDa

  • Ab53496, at a 1/200 dilution, staining Hsp70 + Hsc70 in nuclear smears of rat hepatocytes by immunocytochemistry. 

    1. Control

    2.Five hours after exposure to hyperthermic stress

       

  • ab53496 staining Hsp70 in LLC-PK1 cells expressing Hsp70 under cold block conditions, by Immunocytochemistry/ Immunofluorescence.

    Cells were fixed with 4% paraformaldehyde, permeabilized with 0.01% Triton X-100 in PBS for 4 minutes and blocked with 1% BSA/PBS for 20 minutes. Samples were incubated with primary antibody overnight at 4°C.
  • Overlay histogram showing Jurkat cells stained with ab53496 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53496, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References

This product has been referenced in:

  • Ha J  et al. Hydrogen sulfide inhibits Kir2 and Kir3 channels by decreasing sensitivity to the phospholipid phosphatidylinositol 4,5-bisphosphate (PIP2). J Biol Chem 293:3546-3561 (2018). Read more (PubMed: 29317494) »
  • Tang S  et al. Rosemary Reduces Heat Stress by Inducing CRYAB and HSP70 Expression in Broiler Chickens. Oxid Med Cell Longev 2018:7014126 (2018). Read more (PubMed: 30425783) »
See all 4 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Vielen Dank für Ihre Antwort.

Die Frau Hoffmann ist außer Haus und ich hoffe es ist Ihnen Recht, dass ich mich um Ihren Fall kümmere.

Unser AbTrial ist nur ein Angebot, welches Sie nicht in Anspruch nehmen müssen. Natürlich können Sie jedes unserer Produkte bestellen dass Sie möchten ohne jegliche weitere Verpflichtung.

Ich hoffe dies hilft Ihnen weiter und ich wünsche Ihnen viel Glück mit Ihren Versuchen.

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Answer

Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Leider muss ich Ihnen bestätigen, dass ab53496 und ab79275 nicht in ELISA getestet sind und deshalb auch nicht unserer Garantie unterliegen. Wir wissen auch nicht ob beide Klone das gleiche Epitope binden, da die Epitope nicht gemapped wurden.

Vielleicht finden Sie in unserer Auswahl an anti HSP70 Antikörpern, die schon für ELISA getestet sind ein gutes Pärchen.

Für humane Proben würde ich folgendes vorschlagen:

ab47455 als capture Antikörper und ab48515 als Detektionsantikörper. Prinzipiell ist es von Vorteil einen polyklonalen Detektionsantikörper zu verwenden, da die Signalstärke besser ist und auch eine Epitop-Überlappung weniger wahrscheinlich ist.

Diese zwei Antikörper sind noch nicht in einen sandwichELISA getestet worden. Deshalb würde ich Ihnen folgendes Testprogramm vorschlagen: Abtrial

Die Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link nachlesen:
https://www.abcam.com/abtrial

Bitte lassen Sie mich wissen, ob Sie gerne daran teilnehmen möchten und mit welchen Antikörpern. Ich habe Ihre Bestellung noch nicht weitergegeben.

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Question
Answer

Thank you for contacting us.

The doublets using ab53496are not always well resolved because there is only ˜1kDa between them. The lab was able to provide the attached image showing the doublet on bovine MDBK cells at a 1:1000 dilution. I hope this helps, please let me know if you need any additional information or assistance.

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