Key features and details
- Mouse monoclonal [BB70] to Hsp70
- Suitable for: IHC-P, ICC/IF, Flow Cyt, WB
- Reacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Xenopus laevis, Carp, Drosophila melanogaster, Schizosaccharomyces pombe, Rainbow trout
- Isotype: IgG2a
Product nameAnti-Hsp70 antibody [BB70]
See all Hsp70 primary antibodies
DescriptionMouse monoclonal [BB70] to Hsp70
Tested applicationsSuitable for: IHC-P, ICC/IF, Flow Cyt, WBmore details
Species reactivityReacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Xenopus laevis, Carp, Drosophila melanogaster, Schizosaccharomyces pombe, Rainbow trout
Chicken Hsp70/Hsp90 complex
- WB: Hela cell lysate; Bovine MDBK cell lysate. IHC-P: Mouse and human colon cancer tissues. Flow Cyt: Jurkat cells.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab53496 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|Flow Cyt||Use 0.5µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 70 kDa. Detects 72kDa and 73kDa proteins corresponding to the molecular mass of inducible hsp and hsc70 on SDS PAGE immunoblots.|
RelevanceFunction: In cooperation with other chaperones, the Hsp70 family stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Tissue specificity: HSPA1B is testis-specific.
Cellular localizationCytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
- DnaK type molecular chaperone HSP70 1 antibody
- Epididymis secretory protein Li 103 antibody
- FLJ54303 antibody
ab53496 at 100,000 dilution staining Hsp70 + Hsc70 in mouse colon cancer tissue section by immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). A antibody amplifier™ system was used for staining. A HRP-conjugated secondary antibody was used at 1/10 dilution.
Anti-Hsp70 antibody [BB70] (ab53496) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Predicted band size: 70 kDa
Ab53496, at a dilution of 1/200, staining Hsp70 + Hsc70 in liver smears of rat hepatocytes by Immunohistochemistry Formalin fixed Parafin embedded tissue.
2. Two hours after exposure tp hyperthermic stress
ab53496 at 100,000 dilution staining Hsp70 + Hsc70 in human colon cancer tissue section by immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). A antibody amplifier™ system was used for staining.
Anti-Hsp70 antibody [BB70] (ab53496) at 1/1000 dilution + Bovine MDBK cells
Predicted band size: 70 kDa
Ab53496, at a 1/200 dilution, staining Hsp70 + Hsc70 in nuclear smears of rat hepatocytes by immunocytochemistry.
2.Five hours after exposure to hyperthermic stress
ab53496 staining Hsp70 in LLC-PK1 cells expressing Hsp70 under cold block conditions, by Immunocytochemistry/ Immunofluorescence.
Cells were fixed with 4% paraformaldehyde, permeabilized with 0.01% Triton X-100 in PBS for 4 minutes and blocked with 1% BSA/PBS for 20 minutes. Samples were incubated with primary antibody overnight at 4°C.
Overlay histogram showing Jurkat cells stained with ab53496 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53496, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
ab53496 has been referenced in 4 publications.
- Ha J et al. Hydrogen sulfide inhibits Kir2 and Kir3 channels by decreasing sensitivity to the phospholipid phosphatidylinositol 4,5-bisphosphate (PIP2). J Biol Chem 293:3546-3561 (2018). PubMed: 29317494
- Tang S et al. Rosemary Reduces Heat Stress by Inducing CRYAB and HSP70 Expression in Broiler Chickens. Oxid Med Cell Longev 2018:7014126 (2018). PubMed: 30425783
- Xiao B et al. Endothelial cell-derived exosomes protect SH-SY5Y nerve cells against ischemia/reperfusion injury. Int J Mol Med 40:1201-1209 (2017). PubMed: 28849073
- Rice WL et al. Differential, phosphorylation dependent trafficking of AQP2 in LLC-PK1 cells. PLoS One 7:e32843 (2012). ICC/IF ; Human . PubMed: 22403603