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I have been using ab47455 for a couple years in IP and WB to detect HSP70. It has worked well in the past but two recent lots, GR61173 and GR40351, have not detected exogenous HSP70 or endogenous protein, as had been the case with previous lots. Other antibodies from different suppliers have detected HSP70 in these same samples.
Asked on May 14 2012
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Answered on May 14 2012