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One of the IHC images says that the antibody was diluted 1:100,000. Is that correct, or do you recommend a higher concentration in IHC?
Asked on Sep 06 2012
Thank you very much for your call yesterday and for your patience while I've been in touch with the lab about ab47455.
The antibody was used at a 1:100,000 dilution using a novel antibody amplification system, so these results are not typical and we recommend starting at a 1:1000 dilution.
I am sorry for the confusion, but I hope that this information is useful. Please let me know if you have any questions or if there is anything else that we can do for you.
Answered on Sep 06 2012