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Sorry to bother you, but really need help.
1, Is it applicable in rabbit experiment? I read the specification of the product, but I am still not sure about the question. I mean when I am doing a rabbit experiment, and I want to detect the HSP70 (induced by pressure), not HSP73 (constitutionally existing in cells), is it correct to use this product?
2,Does the HSP70 ( not HSP73) present in normal cells. I mean if I measure the quantity of the HSP70 protein in a totally normal cell, will I get the protein band in Western blot? Will the band be very blurring? Or clear?
3,If I add a traumatic stimulant to the normal rabbit brain, I want to detect the HSP70 protein quantity around the lesion. Will I get the elevated HSP70 result? I read some papers on HSP70, and the elevated HSP70 results were all obtained by whole body overheated not just local injury. So I doubt the effect of the local injury.
Thank you very much for your patience.
Asked on Feb 08 2013
Thank you for contacting us.
Yes, this product has been tested sucessfully in rabbit for Western Blotting. Our results show a clean band.
The antibody should specifically detect HSP70 and not HSP73.
Regarding local injury, I am not sure whether your levels will be elevated or not. Cells that are heat shocked do increase HSP70 so it seems reasonable that this would appear in your staining but this would have to be tested.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Feb 08 2013