Question (71826) | Anti-Hsp70 antibody [C92F3A-5] (ab47455)

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Question

thank you very much for your reply.
One last question, in totally normal cells will I get a band of HSP 70 (not HSP73) in WB? I learned that HSP 73 is constitutionally produced by cells, while HSP70 protein is induced by heat, injury, which makes me to think that HSP70 would not appear in normal cell. Is this correct?














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Dear Sir/Madam








Thank you for contacting us.

Yes, this product has been tested sucessfully in rabbit for Western Blotting. Our results show a clean band.

The antibody should specifically detect HSP70 and not HSP73.

Regarding local injury, I am not sure whether your levels will be elevated or not. Cells that are heat shocked do increase HSP70 so it seems reasonable that this would appear in your staining but this would have to be tested.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.



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Heather

Heather Allen
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Abcam Inc.
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Your original inquiry to Abcam:


Sorry to bother you, but really need help.



I bought the HSP70 antibody from your company. The product is Anti-Hsp70 antibody [C92F3A-5] ab47455.

I have a few questions about the functions of the antibody. Some of the answers to them may seem easy to find on internet, but my english is not good enough, finding answers will be very annoying to me. So I want to ask for help from you. Thank you very much!



1, Is it applicable in rabbit experiment? I read the specification of the product, but I am still not sure about the question. I mean when I am doing a rabbit experiment, and I want to detect the HSP70 (induced by pressure), not HSP73 (constitutionally existing in cells), is it correct to use this product?

2,Does the HSP70 ( not HSP73) present in normal cells. I mean if I measure the quantity of the HSP70 protein in a totally normal cell, will I get the protein band in Western blot? Will the band be very blurring? Or clear?

3,If I add a traumatic stimulant to the normal rabbit brain, I want to detect the HSP70 protein quantity around the lesion. Will I get the elevated HSP70 result? I read some papers on HSP70, and the elevated HSP70 results were all obtained by whole body overheated not just local injury. So I doubt the effect of the local injury.

Thank you very much for your patience.






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Answer

In normal cells there is still some low expression of HSP70, which is upregulated by heat or injury. I hope this information is helpfu.

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