Western blot abreview for Anti-Hsp70 antibody [C92F3A-5]

Excellent
Abreviews
Application
Western blot
Sample
Rat Tissue lysate - whole (Bone tissue (Tibia))
Gel Running Conditions
Reduced Denaturing (12,5%)
Loading amount
50 µg
Specification
Bone tissue (Tibia)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500

Secondary antibody

Dilution
1/1000
Name

Detection

Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 70 kDa
Positive control
sample

Additional data

Abcam response
published PS 25.8.2015

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Submitted Aug 25 2015

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