• Product name

    HSP70 ELISA Kit
    See all HSP70 kits
  • Detection method

  • Precision

    Sample n Mean SD CV%
    Overall 24 < 5%
    Sample n Mean SD CV%
    Overall 9 < 13%
  • Sample type

    Tissue Extracts, Cell Lysate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    200 pg/ml
  • Range

    780 pg/ml - 50000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Tissue Extracts >=90 % - %
    Cell Lysate >=90 % - %

  • Assay time

    4h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Product overview

    HSP70 ELISA kit is designed for the accurate quantitative measurement of HSP70 in samples from Human, Mouse and Rat origins. This assay allows for the quantitative determination of inducible HSP70 from cell lysates and tissue extracts.

    A HSP70 monoclonal antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, incubated and then washed. A HSP70 polyclonal antibody is then added, incubated and washed. An HRP conjugated anti-IgG antibody is then added, incubated. The plate is washed once more and the TMB substrate is then added which HRP catalyzes, generating a blue coloration after incubation. A stop solution is added which generates conversion to yellow color read at 450 nm which is proportional to the amount of analyte bound.

    Get higher sensitivity in only 90 minutes with Human HSP70 ELISA Kit (ab187399) from our SimpleStep ELISA® range.

  • Notes

    Inducible heat shock protein 70 (HSP70) is a stress protein whose expression is upregulated when the cell or organism is placed under conditions of stress. HSP70 is essential for cellular recovery, survival, and maintenance of normal cellular function. It is also a molecular chaperone that prevents protein aggregation and refolds damaged proteins in response to cellular stress caused by environmental insults, pathogens, and disease. Current research is aimed at exploiting HSP70’s cellular protective abilities as a therapeutic strategy against damaging cellular stress.


    In most mammals, the expression of inducible HSP70 is strictly stress inducible and can only be detected following a significant stress upon the cell or organism. However, in humans and primates, inducible HSP70 is present at basal levels and is upregulated in response to stress. The role of HSP70 has been studied in a variety of medically relevant models or conditions such as hyperthermia, hypertension, toxic exposure to chemical agents, hypoxia, ischemia, inflammation, autoimmunity, apoptosis, cancer, organ transplantation, and bacterial and viral infections. HSP70 has also been studied in the normal processes of aging, spermatogenesis, menstruation, and physical activity such as exercise.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform



Associated products


Our Abpromise guarantee covers the use of ab133060 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Representative Standard Curve using ab133060.



This product has been referenced in:

  • Jang KW  et al. Molecular and histological effects of MR-guided pulsed focused ultrasound to the rat heart. J Transl Med 15:252 (2017). Read more (PubMed: 29237455) »
  • Oraby AM  et al. Cognitive Function and Heat Shock Protein 70 in Children With Temporal Lobe Epilepsy. J Child Neurol N/A:N/A (2016). Sandwich ELISA ; Human . Read more (PubMed: 27664194) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

A good kit for use with marine worms

Good Average 3/5 (Ease of Use)
I purchased this kit to try and quantify HSP70 in the marine worm Hediste diversicolor. The kit was fairly straightforward as I have used ELISA kits before although the protocol has a fair amount of typographical errors and the procedure is lacking information about when to add the samples (again fairly easy to work out though). The standard curve produced appears accurate and for my particular samples a 1:2 dilution or 1:4 dilution was necessary to bring the samples in range for testing with this kit. Spiked lysed tissue homogenate samples with HSP70 standard indicated 96% recovery for this species at a 1:4 dilution. I would recommend this product for use with verified or unverified species samples.

Cameron Hird

Verified customer

Submitted Jul 28 2016


Unfortunately, we have not tested this kit on Neisseria meningitidis and Neisseria lactamica lysates. I have compared the total human Hsp70 protein sequence with the Neisseria meningitidis and Neisseria lactamica sequences. Unfortunately, there is only 44% total homology between these species and therefore it is unlikely that this kit will work with your species.

Please also note that this kit will only detect induced Hsp70 and not constitutive Hsp70.

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