Key features and details
- Rabbit polyclonal to Hsp70 (phospho S153)
- Suitable for: WB
- Reacts with: Human
Product nameAnti-Hsp70 (phospho S153) antibody
See all Hsp70 primary antibodies
DescriptionRabbit polyclonal to Hsp70 (phospho S153)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human Hsp70 (phospho S153).
Database link: P0DMV8
- WB: T47D treated with EGF, cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.50
Constituents: 0.24% HEPES, 0.87% Sodium chloride, 50% Glycerol, 0.01% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab228421 is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
Our Abpromise guarantee covers the use of ab228421 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 70 kDa.|
RelevanceFunction: In cooperation with other chaperones, the Hsp70 family stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Tissue specificity: HSPA1B is testis-specific.
Cellular localizationCytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
- DnaK type molecular chaperone HSP70 1 antibody
- Epididymis secretory protein Li 103 antibody
- FLJ54303 antibody
All lanes : Anti-Hsp70 (phospho S153) antibody (ab228421) at 1/1000 dilution
Lane 1 : T-47D (human ductal breast epithelial tumor cell line) cell lysate treated with 1 nM EGF for 60 minutes
Lane 2 : T-47D (human ductal breast epithelial tumor cell line) cell lysate treated with 1 nM EGF for 60 minutes, plus lambda phosphatase (1200 units for 30 minutes)
Developed using the ECL technique.
Predicted band size: 70 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228421 has not yet been referenced specifically in any publications.