• Product name

  • Description

    Rabbit polyclonal to Hsp75
  • Host species

  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Human Hsp75 aa 600-650. The exact sequence is proprietary.
    Database link: Q12931

  • Positive control

    • WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate. IHC-P: Human colon carcinoma tissue.


  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab226401 was affinity purified using an epitope specific to Hsp75 immobilized on solid support.
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab226401 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/25000. Predicted molecular weight: 80 kDa.
IP Use at 2-10 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Function

    Chaperone that expresses an ATPase activity.
  • Tissue specificity

    Found in skeletal muscle, liver, heart, brain, kidney, pancreas, lung and placenta.
  • Sequence similarities

    Belongs to the heat shock protein 90 family.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • Heat Shock Protein 75 antibody
    • Heat shock protein 75 kDa antibody
    • Heat shock protein 75 kDa mitochondrial antibody
    • HSP 75 antibody
    • HSP90L antibody
    • mitochondrial antibody
    • TNF receptor associated protein 1 antibody
    • TNFR Associated Protein antibody
    • TNFR-associated protein 1 antibody
    • TRAP-1 antibody
    • Trap1 antibody
    • TRAP1_HUMAN antibody
    • Tumor Necrosis Factor Receptor Associated Protein 1 antibody
    • Tumor necrosis factor type 1 receptor associated protein antibody
    • Tumor necrosis factor type 1 receptor-associated protein antibody
    see all


  • All lanes : Anti-Hsp75 antibody (ab226401) at 0.04 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 80 kDa

    Exposure time: 10 seconds

    Lysates prepared using NETN lysis buffer.

  • Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for Hsp75 using ab226401 at 1/1000 dilution in immunohistochemical analysis.

    Detection: DAB staining.

  • Hsp75 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226401 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226401 at 0.4 µg/ml.

    Lane 1: ab226401 IP in HEK-293T whole cell lysate.

    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection: Chemiluminescence with exposure time of 10 seconds.


ab226401 has not yet been referenced specifically in any publications.

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