Anti-Hsp90 alpha antibody [5G5] (ab128483)

Mouse monoclonal Hsp90 alpha antibody [5G5]. Validated in WB, ELISA, IHC, Flow Cyt and tested in Mouse, Human, Monkey. Immunogen corresponding to recombinant fragment.

Overview

  • Product name

    Anti-Hsp90 alpha antibody [5G5]
    See all Hsp90 alpha primary antibodies
  • Description

    Mouse monoclonal [5G5] to Hsp90 alpha
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ELISA, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Human, Monkey
  • Immunogen

    Purified recombinant fragment of Human Hsp90 alpha expressed in E. coli.

  • Positive control

    • Human Hsp90 alpha (amino acids 275-484) recombinant protein; NIH 3T3, HeLa, HCT116, HL60, SK-Br-3, C6 and C0S7 cell lysates
      Flow Cyt: HeLa cells
      IHC-P: Lung cancer tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab128483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 85 kDa.
ELISA 1/10000.
IHC-P 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Molecular chaperone. Has ATPase activity.
  • Sequence similarities

    Belongs to the heat shock protein 90 family.
  • Post-translational
    modifications

    ISGylated.
  • Cellular localization

    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • EL52 antibody
    • epididymis luminal secretory protein 52 antibody
    • Heat shock 86 kDa antibody
    • heat shock 90kD protein 1, alpha antibody
    • Heat shock 90kD protein 1, alpha like 4 antibody
    • heat shock 90kD protein, alpha-like 4 antibody
    • Heat shock 90kDa protein 1 alpha antibody
    • Heat shock protein 90kDa alpha (cytosolic) class A member 1 antibody
    • Heat shock protein HSP 90-alpha antibody
    • HS90A_HUMAN antibody
    • HSP 86 antibody
    • HSP86 antibody
    • Hsp89 antibody
    • HSP89A antibody
    • Hsp90 antibody
    • HSP90A antibody
    • HSP90AA1 antibody
    • HSP90ALPHA antibody
    • HSP90N antibody
    • HSPC1 antibody
    • HSPCA antibody
    • HSPCAL1 antibody
    • HSPCAL4 antibody
    • HSPN antibody
    • LAP 2 antibody
    • LAP2 antibody
    • lipopolysaccharide-associated protein 2 antibody
    • LPS-associated protein 2 antibody
    • Renal carcinoma antigen NY-REN-38 antibody
    see all

Images

  • Anti-Hsp90 alpha antibody [5G5] (ab128483) at 1/500 dilution + Human Hsp90 alpha (amino acids 275-484) recombinant protein

    Predicted band size: 85 kDa



    Expected MWt of recombinant protein is 50.5 kDa.
  • ELISA using ab128483.
  • All lanes : Anti-Hsp90 alpha antibody [5G5] (ab128483) at 1/500 dilution

    Lane 1 : NIH 3T3 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HCT116 cell lysate
    Lane 4 : HL-60 cell lysate
    Lane 5 : SK-Br-3 cell lysate
    Lane 6 : C6 cell lysate
    Lane 7 : C0S7 cell lysate

    Predicted band size: 85 kDa

  • ab128483, at a 1/200 dilution, staining Hsp90 alpha in HeLa cells by Flow Cytometry (green). Negative control is shown in red.
  • ab128483, at a 1/200 dilution, staining Hsp90 alpha in paraffin embedded lung cancer tissue by Immunohistochemistry, with DAB staining.
  • ab128483, at a 1/200 dilution, staining Hsp90 alpha in paraffin embedded lung cancer tissue by Immunohistochemistry, with DAB staining.

References

ab128483 has not yet been referenced specifically in any publications.

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