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Our Abpromise guarantee covers the use of ab74248 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 5 - 10 µg/ml. Predicted molecular weight: 85 kDa.
Use at a concentration of 5 - 10 µg/ml for 2 hours at room temperature.
|IP||Use at 10 µg/mg of lysate.
(Native verified, use Protein A)
|IHC-P||Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at a concentration of 5 - 10 µg/ml for 30 minutes at room temperature. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 to 20 minutes followed by cooling at room temperature for 20 minutes.
|ICC/IF||Use at an assay dependent concentration.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"