Anti-Hsp90 antibody [4F3.E8] (ab79848)
Key features and details
- Mouse monoclonal [4F3.E8] to Hsp90
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2a
Overview
-
Product name
Anti-Hsp90 antibody [4F3.E8]
See all Hsp90 primary antibodies -
Description
Mouse monoclonal [4F3.E8] to Hsp90 -
Host species
Mouse -
Specificity
ab79848 detects both alpha and beta forms of Hsp90 equally well. -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant Human Hsp90 purified from E.coli
-
Positive control
- Heat shocked HeLa cell lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
4F3.E8 -
Isotype
IgG2a -
Research areas
Associated products
-
Compatible Secondaries
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab79848 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/100.
|
|
WB | (1) |
1/2000. Predicted molecular weight: 85 , 83 kDa.
|
ICC/IF |
Use at an assay dependent concentration.
|
Notes |
---|
IHC-P
1/100. |
WB
1/2000. Predicted molecular weight: 85 , 83 kDa. |
ICC/IF
Use at an assay dependent concentration. |
Target
-
Function
Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. -
Sequence similarities
Belongs to the heat shock protein 90 family. -
Domain
The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins like the co-chaperone STUB1. -
Post-translational
modificationsISGylated.
S-nitrosylated; negatively regulates the ATPase activity and the activation of eNOS by HSP90AA1. -
Cellular localization
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
-
Database links
- Entrez Gene: 3320 Human
- Entrez Gene: 3326 Human
- Entrez Gene: 15516 Mouse
- Entrez Gene: 15519 Mouse
- Entrez Gene: 299331 Rat
- Entrez Gene: 301252 Rat
- Omim: 140571 Human
- Omim: 140572 Human
see all -
Alternative names
- Heat shock 86 kDa antibody
- Heat shock protein 90kDa alpha cytosolic class A member 1 antibody
- Heat shock protein 90kDa alpha cytosolic class B member 1 antibody
see all
Images
-
Anti-Hsp90 antibody [4F3.E8] (ab79848) at 1/1000 dilution + Rat tissue lysate
Predicted band size: 85 , 83 kDa -
ab79848 staining Hsp90 in Mouse backskin tissue sections by ICC/IF (Immunocytochemistry/immunofluorescence).
-
ab79848 staining Hsp90 in Human keratinocyte cell line by ICC/IF (Immunocytochemistry/immunofluorescence).
-
ab79848 staining Hsp90 in Human colon cancer tissue sections by Immunohistochemistry. Samples were incubated with primary antibody at 1:100,000 dilution for 12hours at 4ºC. A Biotin Goat anti-mouse was used as the secondary antibody at 1/2000 dilution for 1 hour at room temperature. Mayer Hematoxylin was used as a nuclear stain.
-
ab79848 at 100 dilution staining Hsp90 in mouse muscle tissue by Immunohistochemistry (Bouin's fixed paraffin embedded tissue sections). Antigen retrieval using microwave method and citrate buffer. A Fluorophore conjugated goat anti mouse at 1/50 dilution was used as secondary.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab79848 has been referenced in 1 publication.
- Halder LD et al. Immune modulation by complement receptor 3-dependent human monocyte TGF-ß1-transporting vesicles. Nat Commun 11:2331 (2020). PubMed: 32393780