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Recombinant Human Hsp90 purified from E.coli
Our Abpromise guarantee covers the use of ab79848 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|WB||1/2000. Predicted molecular weight: 85 , 83 kDa.|
|IP||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
ab79848 staining Hsp90 in Mouse backskin tissue sections by ICC/IF (Immunocytochemistry/immunofluorescence).
ab79848 staining Hsp90 in Human keratinocyte cell line by ICC/IF (Immunocytochemistry/immunofluorescence).
ab79848 staining Hsp90 in Human colon cancer tissue sections by Immunohistochemistry. Samples were incubated with primary antibody at 1:100,000 dilution for 12hours at 4ºC. A Biotin Goat anti-mouse was used as the secondary antibody at 1/2000 dilution for 1 hour at room temperature. Mayer Hematoxylin was used as a nuclear stain.
ab79848 at 100 dilution staining Hsp90 in mouse muscle tissue by Immunohistochemistry (Bouin's fixed paraffin embedded tissue sections). Antigen retrieval using microwave method and citrate buffer. A Fluorophore conjugated goat anti mouse at 1/50 dilution was used as secondary.
ab79848 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"