• Product name

    Anti-Hsp90 antibody [4F3.E8]
    See all Hsp90 primary antibodies
  • Description

    Mouse monoclonal [4F3.E8] to Hsp90
  • Host species

  • Specificity

    ab79848 detects both alpha and beta forms of Hsp90 equally well.
  • Tested applications

    Suitable for: IHC-P, IHC-Fr, WB, IP, ICC, ICC/IF, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant Human Hsp90 purified from E.coli

  • Positive control

    • Heat shocked HeLa cell lysate.



Our Abpromise guarantee covers the use of ab79848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.
IHC-Fr Use at an assay dependent concentration.
WB 1/2000. Predicted molecular weight: 85 , 83 kDa.
IP Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.


  • Function

    Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.
  • Sequence similarities

    Belongs to the heat shock protein 90 family.
  • Domain

    The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins like the co-chaperone STUB1.
  • Post-translational

    S-nitrosylated; negatively regulates the ATPase activity and the activation of eNOS by HSP90AA1.
  • Cellular localization

    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • Heat shock 86 kDa antibody
    • Heat shock protein 90kDa alpha cytosolic class A member 1 antibody
    • Heat shock protein 90kDa alpha cytosolic class B member 1 antibody
    • Heat shock protein HSP 90 alpha antibody
    • Heat shock protein HSP 90 beta antibody
    • Heat shock protein HSP 90-alpha antibody
    • HS90A_HUMAN antibody
    • HSP 84 antibody
    • HSP 86 antibody
    • Hsp 90 antibody
    • HSP86 antibody
    • HSP90A antibody
    • HSP90AA1 antibody
    • HSP90AB1 antibody
    • HSP90B antibody
    • HSPC1 antibody
    • HSPC2 antibody
    • HSPCAL1 antibody
    • HSPCAL4 antibody
    • Renal carcinoma antigen NY-REN-38 antibody
    see all


  • Anti-Hsp90 antibody [4F3.E8] (ab79848) at 1/1000 dilution + Rat tissue lysate

    Predicted band size: 85 , 83 kDa

  • ab79848 staining Hsp90 in Mouse backskin tissue sections by ICC/IF (Immunocytochemistry/immunofluorescence).

  • ab79848 staining Hsp90 in Human keratinocyte cell line by ICC/IF (Immunocytochemistry/immunofluorescence).

  • ab79848 staining Hsp90 in Human colon cancer tissue sections by Immunohistochemistry. Samples were incubated with primary antibody at 1:100,000 dilution for 12hours at 4ºC. A Biotin Goat anti-mouse was used as the secondary antibody at 1/2000 dilution for 1 hour at room temperature. Mayer Hematoxylin was used as a nuclear stain. 

  • ab79848 at 100 dilution staining Hsp90 in mouse muscle tissue by Immunohistochemistry (Bouin's fixed paraffin embedded tissue sections). Antigen retrieval using microwave method and citrate buffer. A Fluorophore conjugated goat anti mouse at 1/50 dilution was used as secondary.


ab79848 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Western blot
Human Cell lysate - whole cell (HK-2 cells (Human Kidney))
Loading amount
9 µg
HK-2 cells (Human Kidney)
Gel Running Conditions
Reduced Denaturing (Nupage 4-12% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 16°C

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