Anti-Hsp90 antibody (ab13495)
Key features and details
- Rabbit polyclonal to Hsp90
- Suitable for: ICC/IF, IHC-P, ICC, IHC-Fr, ELISA, IP, Flow Cyt, WB
- Reacts with: Mouse, Rat, Human, Xenopus laevis
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Hsp90 antibody
See all Hsp90 primary antibodies -
Description
Rabbit polyclonal to Hsp90 -
Host species
Rabbit -
Specificity
Detects 90kD proteins corresponding to the molecular mass of hsp90aß. -
Tested applications
Suitable for: ICC/IF, IHC-P, ICC, IHC-Fr, ELISA, IP, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Xenopus laevis -
Immunogen
Human Hsp90.
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Positive control
- ICC: Heat Shocked Cervical cancer cell line (HeLa).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab13495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (3) |
1/100.
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| IHC-P | (1) |
Use at an assay dependent concentration.
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| ICC | (1) |
Use at an assay dependent concentration.
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| IHC-Fr |
Use at an assay dependent concentration.
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| ELISA |
Use at an assay dependent concentration.
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| IP | (1) |
Use at an assay dependent concentration.
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| Flow Cyt | (1) |
Use at an assay dependent concentration.
See Abreview. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
| WB | (7) |
1/20000 - 1/40000. Detects a band of approximately 90 kDa (predicted molecular weight: 83.2 (beta) , 84.5 (alpha) kDa).
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| Notes |
|---|
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ICC/IF
1/100. |
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IHC-P
Use at an assay dependent concentration. |
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ICC
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
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ELISA
Use at an assay dependent concentration. |
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IP
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent concentration. See Abreview. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB
1/20000 - 1/40000. Detects a band of approximately 90 kDa (predicted molecular weight: 83.2 (beta) , 84.5 (alpha) kDa). |
Target
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Function
Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. -
Sequence similarities
Belongs to the heat shock protein 90 family. -
Domain
The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins like the co-chaperone STUB1. -
Post-translational
modificationsISGylated.
S-nitrosylated; negatively regulates the ATPase activity and the activation of eNOS by HSP90AA1. -
Cellular localization
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 3320 Human
- Entrez Gene: 3326 Human
- Entrez Gene: 15516 Mouse
- Entrez Gene: 15519 Mouse
- Entrez Gene: 299331 Rat
- Entrez Gene: 301252 Rat
- Omim: 140571 Human
- Omim: 140572 Human
see all -
Alternative names
- Heat shock 86 kDa antibody
- Heat shock protein 90kDa alpha cytosolic class A member 1 antibody
- Heat shock protein 90kDa alpha cytosolic class B member 1 antibody
see all
Images
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Western blot - Anti-Hsp90 antibody (ab13495)All lanes : Anti-Hsp90 antibody (ab13495) at 1/10000 dilution (2 hours at room temperature)
Lane 1 : A431 with BSA for 30 minutes at room temperature
Lane 2 : A549 with BSA for 30 minutes at room temperature
Lane 3 : HCT116 with BSA for 30 minutes at room temperature
Lane 4 : Hela with BSA for 30 minutes at room temperature
Lane 5 : HEK293 with BSA for 30 minutes at room temperature
Lane 6 : HepG2 with BSA for 30 minutes at room temperature
Lane 7 : HL-60 with BSA for 30 minutes at room temperature
Lane 8 : HUVEC with BSA for 30 minutes at room temperature
Lane 9 : Jurkat with BSA for 30 minutes at room temperature
Lane 10 : MCF7 with BSA for 30 minutes at room temperature
Lane 11 : PC3 with BSA for 30 minutes at room temperature
Lane 12 : T98G with BSA for 30 minutes at room temperature
Lysates/proteins at 2 µg per lane.
Blocking peptides at 1.5 % per lane.
Secondary
All lanes : HRP Donkey anti-rabbit IgG, 1 hour at room temperature
Predicted band size: 83.2 (beta) , 84.5 (alpha) kDa -
Western blot - Anti-Hsp90 antibody (ab13495)Image courtesy of Dr Mahesh Shivananjappa by Abreview.Anti-Hsp90 antibody (ab13495) at 1/1000 dilution + whole cell lysate prepared from human platelets treated with A23187 for 1 hour at 20 µg
Secondary
HRP conjugated goat anti-rabbit polyclonal at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 83.2 (beta) , 84.5 (alpha) kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Additional bands at: 36 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 antibody (ab13495)ab13495 staining Hsp90 in Mouse backskin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with Bouin's fixative solution. Samples were incubated with primary antibody (1/100) for 1 hour at room temperature. A FITC-conjugated Goat anti-rabbit IgG polyclonal (1/50) was used as the secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 antibody (ab13495)Immunofluorescent analysis of 2% paraformaldehyde fixed Heat Shocked Cervical cancer cell line (HeLa) for 20min at RT, labelling Hsp90 with ab13495 at 1/100 dilution for 12 hours at 4°C, followed by secondary antibody Goat Anti-Rabbit (FITC) (green) at 1/200 dilution for 2 hours at RT. Image showing cytoplasmic staining on HeLa cells. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp90 Antibody (green) (C) Composite image.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (49)
ab13495 has been referenced in 49 publications.
- Niu L et al. Sericin inhibits MDA-MB-468 cell proliferation via the PI3K/Akt pathway in triple-negative breast cancer. Mol Med Rep 23:N/A (2021). PubMed: 33313947
- Casagrande BP et al. AMPK in the gut-liver-brain axis and its influence on OP rats in an HSHF intake and WTD rat model. Pflugers Arch 473:1199-1211 (2021). PubMed: 34075446
- Mesquita PHC et al. Effects of Resistance Training on the Redox Status of Skeletal Muscle in Older Adults. Antioxidants (Basel) 10:N/A (2021). PubMed: 33652958
- Chiang S et al. Mechanisms of impaired mitochondrial homeostasis and NAD+ metabolism in a model of mitochondrial heart disease exhibiting redox active iron accumulation. Redox Biol 46:102038 (2021). PubMed: 34416478
- Uda M et al. Potential roles of neuronal nitric oxide synthase and the PTEN-induced kinase 1 (PINK1)/Parkin pathway for mitochondrial protein degradation in disuse-induced soleus muscle atrophy in adult rats. PLoS One 15:e0243660 (2020). PubMed: 33296434