Product nameAnti-Hsp90 antibody
See all Hsp90 primary antibodies
DescriptionRabbit polyclonal to Hsp90
SpecificityDetects 90kD proteins corresponding to the molecular mass of hsp90aß.
Tested applicationsSuitable for: ELISA, IP, IHC-P, IHC-Fr, ICC/IF, ICC, Flow Cyt, WBmore details
Species reactivityReacts with: Mouse, Rat, Human, Xenopus laevis
- RAW 264.7 whole cell lysate (ab7187) can be used as a positive control in WB. HeLa Cell Lysate (Heat Shocked). Hsp90 Protein.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: Whole serum
Our Abpromise guarantee covers the use of ab13495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/20000 - 1/40000. Detects a band of approximately 90 kDa (predicted molecular weight: 83.2 (beta) , 84.5 (alpha) kDa).|
FunctionMolecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.
Sequence similaritiesBelongs to the heat shock protein 90 family.
DomainThe TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins like the co-chaperone STUB1.
S-nitrosylated; negatively regulates the ATPase activity and the activation of eNOS by HSP90AA1.
Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- Heat shock 86 kDa antibody
- Heat shock protein 90kDa alpha cytosolic class A member 1 antibody
- Heat shock protein 90kDa alpha cytosolic class B member 1 antibody
All lanes : Anti-Hsp90 antibody (ab13495) at 1/10000 dilution (2 hours at room temperature)
Lane 1 : A431 with BSA for 30 minutes at room temperature
Lane 2 : A549 with BSA for 30 minutes at room temperature
Lane 3 : HCT116 with BSA for 30 minutes at room temperature
Lane 4 : Hela with BSA for 30 minutes at room temperature
Lane 5 : HEK293 with BSA for 30 minutes at room temperature
Lane 6 : HepG2 with BSA for 30 minutes at room temperature
Lane 7 : HL-60 with BSA for 30 minutes at room temperature
Lane 8 : HUVEC with BSA for 30 minutes at room temperature
Lane 9 : Jurkat with BSA for 30 minutes at room temperature
Lane 10 : MCF7 with BSA for 30 minutes at room temperature
Lane 11 : PC3 with BSA for 30 minutes at room temperature
Lane 12 : T98G with BSA for 30 minutes at room temperature
Lysates/proteins at 2 µg per lane.
Blocking peptides at 1.5 % per lane.
All lanes : HRP Donkey anti-rabbit IgG, 1 hour at room temperature
Predicted band size: 83.2 (beta) , 84.5 (alpha) kDa
ab13495 staining Hsp90 in murine RAW 264 (ab7187).7 cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton-X100 in 2% BSA for 15 minutes, blocked with 2% BSA for 1 hour at 4°C and then incubated with ab13495 at a 1/150 dilution. The secondary used was an Alexa-Fluor 488 conjugated chicken anti-rabbit IgG (H+L) used at a 1/500 dilution.
ab13495 staining Hsp90 in Mouse backskin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with Bouin's fixative solution. Samples were incubated with primary antibody (1/100) for 1 hour at room temperature. A FITC-conjugated Goat anti-rabbit IgG polyclonal (1/50) was used as the secondary antibody.
ab13495 at a 1/100 dilution staining Hsp90 in human PMN cells by Immunocytochemistry/ Immunofluorescence incubated for 4 hours at 37°C. PFA fixed. Blocked using 2% BSA for 1 hour at 22°C. Secondary used at 1/250 polyclonal Goat anti-rabbit IgG (H+L) conjugated to Alexa Fluor 568 (ab175471).Left image: DAPI staining nuclei (blue)Middle image: Hsp90 (red)Right image: Overlay
ab13495 staining Hsp90 in Human platelet cells by Flow cytometry.
Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton-X-100 in 2% BSA for 15 minutes. Primary antibody used at a 1/250 dilution and incubated for 18 hours at 4°C. The secondary antibody used was an Alexa Fluor®488 conjugated chicken anti-rabbit IgG (H+L) at a 1/500 dilution.
P : Permeabilized; US : Unstained, Red Peak; IgG RB : IgG Rabbit (Isotype Control) ab171870), Blue Peak; HSP90, Green peak.
Anti-Hsp90 antibody (ab13495) at 1/1000 dilution + whole cell lysate prepared from human platelets treated with A23187 for 1 hour at 20 µg
HRP conjugated goat anti-rabbit polyclonal at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 83.2 (beta) , 84.5 (alpha) kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Additional bands at: 36 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
This product has been referenced in:
- Cho S et al. Progerin phosphorylation in interphase is lower and less mechanosensitive than lamin-A,C in iPS-derived mesenchymal stem cells. Nucleus 9:230-245 (2018). Read more (PubMed: 29619860) »
- An T et al. Comparative analysis of proteomes between diabetic and normal human sperm: Insights into the effects of diabetes on male reproduction based on the regulation of mitochondria-related proteins. Mol Reprod Dev 85:7-16 (2018). Read more (PubMed: 29149484) »