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  1. Link

    hsp90-antibody-ab13495.pdf

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Signal Transduction Protein Trafficking Chaperones Heat Shock Proteins
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Anti-Hsp90 antibody (ab13495)

  • Datasheet
  • SDS
Reviews (14)Q&A (7)References (49)

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Western blot - Anti-Hsp90 antibody (ab13495)
  • Western blot - Anti-Hsp90 antibody (ab13495)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 antibody (ab13495)
  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 antibody (ab13495)

Key features and details

  • Rabbit polyclonal to Hsp90
  • Suitable for: ICC/IF, IHC-P, ICC, IHC-Fr, ELISA, IP, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human, Xenopus laevis
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Hsp90 antibody [EPR3953] (ab109248)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Hsp90 antibody
    See all Hsp90 primary antibodies
  • Description

    Rabbit polyclonal to Hsp90
  • Host species

    Rabbit
  • Specificity

    Detects 90kD proteins corresponding to the molecular mass of hsp90aß.
  • Tested applications

    Suitable for: ICC/IF, IHC-P, ICC, IHC-Fr, ELISA, IP, Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Xenopus laevis
  • Immunogen

    Human Hsp90.

  • Positive control

    • ICC: Heat Shocked Cervical cancer cell line (HeLa).
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Heat Shock Proteins
    • Cancer
    • Tumor biomarkers
    • Other
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568) (ab175471)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • RAW 264.7 whole cell lysate (ab7187)
  • Recombinant Protein

    • Recombinant P. falciparum HSP90 protein (ab222790)
  • Related Products

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab13495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (3)
1/100.
IHC-P (1)
Use at an assay dependent concentration.
ICC (1)
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
IP (1)
Use at an assay dependent concentration.
Flow Cyt (1)
Use at an assay dependent concentration.

See Abreview. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

WB (7)
1/20000 - 1/40000. Detects a band of approximately 90 kDa (predicted molecular weight: 83.2 (beta) , 84.5 (alpha) kDa).
Notes
ICC/IF
1/100.
IHC-P
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

See Abreview. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/20000 - 1/40000. Detects a band of approximately 90 kDa (predicted molecular weight: 83.2 (beta) , 84.5 (alpha) kDa).

Target

  • Function

    Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.
  • Sequence similarities

    Belongs to the heat shock protein 90 family.
  • Domain

    The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins like the co-chaperone STUB1.
  • Post-translational
    modifications

    ISGylated.
    S-nitrosylated; negatively regulates the ATPase activity and the activation of eNOS by HSP90AA1.
  • Cellular localization

    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession P07900 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3320 Human
    • Entrez Gene: 3326 Human
    • Entrez Gene: 15516 Mouse
    • Entrez Gene: 15519 Mouse
    • Entrez Gene: 299331 Rat
    • Entrez Gene: 301252 Rat
    • Omim: 140571 Human
    • Omim: 140572 Human
    • SwissProt: P07900 Human
    • SwissProt: P08238 Human
    • SwissProt: P07901 Mouse
    • SwissProt: P11499 Mouse
    • SwissProt: P34058 Rat
    • SwissProt: P82995 Rat
    • Unigene: 525600 Human
    • Unigene: 1843 Mouse
    • Unigene: 315997 Mouse
    • Unigene: 341186 Mouse
    • Unigene: 440626 Mouse
    • Unigene: 486334 Mouse
    • Unigene: 119867 Rat
    • Unigene: 232226 Rat
    • Unigene: 3277 Rat
    see all
  • Alternative names

    • Heat shock 86 kDa antibody
    • Heat shock protein 90kDa alpha cytosolic class A member 1 antibody
    • Heat shock protein 90kDa alpha cytosolic class B member 1 antibody
    • Heat shock protein HSP 90 alpha antibody
    • Heat shock protein HSP 90 beta antibody
    • Heat shock protein HSP 90-alpha antibody
    • HS90A_HUMAN antibody
    • HSP 84 antibody
    • HSP 86 antibody
    • Hsp 90 antibody
    • HSP86 antibody
    • HSP90A antibody
    • HSP90AA1 antibody
    • HSP90AB1 antibody
    • HSP90B antibody
    • HSPC1 antibody
    • HSPC2 antibody
    • HSPCAL1 antibody
    • HSPCAL4 antibody
    • Renal carcinoma antigen NY-REN-38 antibody
    see all

Images

  • Western blot - Anti-Hsp90 antibody (ab13495)
    Western blot - Anti-Hsp90 antibody (ab13495)
    All lanes : Anti-Hsp90 antibody (ab13495) at 1/10000 dilution (2 hours at room temperature)

    Lane 1 : A431 with BSA for 30 minutes at room temperature
    Lane 2 : A549 with BSA for 30 minutes at room temperature
    Lane 3 : HCT116 with BSA for 30 minutes at room temperature
    Lane 4 : Hela with BSA for 30 minutes at room temperature
    Lane 5 : HEK293 with BSA for 30 minutes at room temperature
    Lane 6 : HepG2 with BSA for 30 minutes at room temperature
    Lane 7 : HL-60 with BSA for 30 minutes at room temperature
    Lane 8 : HUVEC with BSA for 30 minutes at room temperature
    Lane 9 : Jurkat with BSA for 30 minutes at room temperature
    Lane 10 : MCF7 with BSA for 30 minutes at room temperature
    Lane 11 : PC3 with BSA for 30 minutes at room temperature
    Lane 12 : T98G with BSA for 30 minutes at room temperature

    Lysates/proteins at 2 µg per lane.

    Blocking peptides at 1.5 % per lane.

    Secondary
    All lanes : HRP Donkey anti-rabbit IgG, 1 hour at room temperature

    Predicted band size: 83.2 (beta) , 84.5 (alpha) kDa

  • Western blot - Anti-Hsp90 antibody (ab13495)
    Western blot - Anti-Hsp90 antibody (ab13495)Image courtesy of Dr Mahesh Shivananjappa by Abreview.
    Anti-Hsp90 antibody (ab13495) at 1/1000 dilution + whole cell lysate prepared from human platelets treated with A23187 for 1 hour at 20 µg

    Secondary
    HRP conjugated goat anti-rabbit polyclonal at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 83.2 (beta) , 84.5 (alpha) kDa
    Observed band size: 90 kDa why is the actual band size different from the predicted?
    Additional bands at: 36 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 5 minutes

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 antibody (ab13495)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 antibody (ab13495)

    ab13495 staining Hsp90 in Mouse backskin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with Bouin's fixative solution. Samples were incubated with primary antibody (1/100) for 1 hour at room temperature. A FITC-conjugated Goat anti-rabbit IgG polyclonal (1/50) was used as the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 antibody (ab13495)
    Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 antibody (ab13495)

    Immunofluorescent analysis of 2% paraformaldehyde fixed Heat Shocked Cervical cancer cell line (HeLa) for 20min at RT, labelling Hsp90 with ab13495 at 1/100 dilution for 12 hours at 4°C, followed by secondary antibody Goat Anti-Rabbit (FITC) (green) at 1/200 dilution for 2 hours at RT. Image showing cytoplasmic staining on HeLa cells. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp90 Antibody (green) (C) Composite image.

Protocols

  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (49)

Publishing research using ab13495? Please let us know so that we can cite the reference in this datasheet.

ab13495 has been referenced in 49 publications.

  • Niu L  et al. Sericin inhibits MDA-MB-468 cell proliferation via the PI3K/Akt pathway in triple-negative breast cancer. Mol Med Rep 23:N/A (2021). PubMed: 33313947
  • Casagrande BP  et al. AMPK in the gut-liver-brain axis and its influence on OP rats in an HSHF intake and WTD rat model. Pflugers Arch 473:1199-1211 (2021). PubMed: 34075446
  • Mesquita PHC  et al. Effects of Resistance Training on the Redox Status of Skeletal Muscle in Older Adults. Antioxidants (Basel) 10:N/A (2021). PubMed: 33652958
  • Chiang S  et al. Mechanisms of impaired mitochondrial homeostasis and NAD+ metabolism in a model of mitochondrial heart disease exhibiting redox active iron accumulation. Redox Biol 46:102038 (2021). PubMed: 34416478
  • Uda M  et al. Potential roles of neuronal nitric oxide synthase and the PTEN-induced kinase 1 (PINK1)/Parkin pathway for mitochondrial protein degradation in disuse-induced soleus muscle atrophy in adult rats. PLoS One 15:e0243660 (2020). PubMed: 33296434
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-3 of 3 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Hsp90 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Human Cell (U87MG Human brain glioblastoma)
Specification
U87MG Human brain glioblastoma
Permeabilization
Yes - 0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
Fixative
Paraformaldehyde
Read More

DR. Dimitra Kalamida

Verified customer

Submitted Nov 18 2013

Immunocytochemistry/ Immunofluorescence abreview for Anti-Hsp90 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cultured Cells (RAW 264.7)
Specification
RAW 264.7
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton-X100 in 2% BSA for 15min
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C
Read More

Dr. Mahesh Shivananjappa

Verified customer

Submitted Jun 21 2012

Immunocytochemistry/ Immunofluorescence abreview for Anti-Hsp90 antibody

Below Average
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (neuronal)
Specification
neuronal
Fixative
Methanol
Permeabilization
Yes - 0.2% Triton x-100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Read More

Abcam user community

Verified customer

Submitted Apr 03 2012

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