Anti-Hsp90 antibody (ab13495)
- Datasheet
- References (22)
- Protocols
Overview
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Product nameAnti-Hsp90 antibody
See all Hsp90 primary antibodies -
DescriptionRabbit polyclonal to Hsp90
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Host speciesRabbit
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SpecificityDetects 90kD proteins corresponding to the molecular mass of hsp90aß.
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Tested applicationsSuitable for: ELISA, IP, IHC-P, IHC-Fr, ICC/IF, ICC, Flow Cyt, WBmore details
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Species reactivityReacts with: Mouse, Rat, Human, Xenopus laevis
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Immunogen
Human Hsp90.
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Positive control
- RAW 264.7 whole cell lysate (ab7187) can be used as a positive control in WB. HeLa Cell Lysate (Heat Shocked). Hsp90 Protein.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferConstituent: Whole serum
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PurityWhole antiserum
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Corresponding Antibody
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab13495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. | |
| ICC | Use at an assay dependent concentration. | |
| Flow Cyt | 1/250. (see Abreview). ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB | 1/20000 - 1/40000. Detects a band of approximately 90 kDa (predicted molecular weight: 83.2 (beta) , 84.5 (alpha) kDa). |
Target
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FunctionMolecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.
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Sequence similaritiesBelongs to the heat shock protein 90 family.
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DomainThe TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins like the co-chaperone STUB1.
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Post-translational
modificationsISGylated.
S-nitrosylated; negatively regulates the ATPase activity and the activation of eNOS by HSP90AA1. -
Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
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Database links
- Entrez Gene: 3320 Human
- Entrez Gene: 3326 Human
- Entrez Gene: 15516 Mouse
- Entrez Gene: 15519 Mouse
- Entrez Gene: 299331 Rat
- Entrez Gene: 301252 Rat
- Omim: 140571 Human
- Omim: 140572 Human
see all -
Alternative names
- Heat shock 86 kDa antibody
- Heat shock protein 90kDa alpha cytosolic class A member 1 antibody
- Heat shock protein 90kDa alpha cytosolic class B member 1 antibody
see all
Images
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Western blot - Anti-Hsp90 antibody (ab13495)All lanes : Anti-Hsp90 antibody (ab13495) at 1/10000 dilution (2 hours at room temperature)
Lane 1 : A431 with BSA for 30 minutes at room temperature
Lane 2 : A549 with BSA for 30 minutes at room temperature
Lane 3 : HCT116 with BSA for 30 minutes at room temperature
Lane 4 : Hela with BSA for 30 minutes at room temperature
Lane 5 : HEK293 with BSA for 30 minutes at room temperature
Lane 6 : HepG2 with BSA for 30 minutes at room temperature
Lane 7 : HL-60 with BSA for 30 minutes at room temperature
Lane 8 : HUVEC with BSA for 30 minutes at room temperature
Lane 9 : Jurkat with BSA for 30 minutes at room temperature
Lane 10 : MCF7 with BSA for 30 minutes at room temperature
Lane 11 : PC3 with BSA for 30 minutes at room temperature
Lane 12 : T98G with BSA for 30 minutes at room temperature
Lysates/proteins at 2 µg per lane.
Blocking peptides at 1.5 % per lane.
Secondary
All lanes : HRP Donkey anti-rabbit IgG, 1 hour at room temperature
Predicted band size: 83.2 (beta) , 84.5 (alpha) kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 antibody (ab13495)Image courtesy of Dr Mahesh Shivananjappa by Abreview.ab13495 staining Hsp90 in murine RAW 264 (ab7187).7 cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton-X100 in 2% BSA for 15 minutes, blocked with 2% BSA for 1 hour at 4°C and then incubated with ab13495 at a 1/150 dilution. The secondary used was an Alexa-Fluor 488 conjugated chicken anti-rabbit IgG (H+L) used at a 1/500 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 antibody (ab13495)ab13495 staining Hsp90 in Mouse backskin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with Bouin's fixative solution. Samples were incubated with primary antibody (1/100) for 1 hour at room temperature. A FITC-conjugated Goat anti-rabbit IgG polyclonal (1/50) was used as the secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 antibody (ab13495)This image was kindly supplied by Dr Mahesh Shivananjappa by Abreviewab13495 at a 1/100 dilution staining Hsp90 in human PMN cells by Immunocytochemistry/ Immunofluorescence incubated for 4 hours at 37°C. PFA fixed. Blocked using 2% BSA for 1 hour at 22°C. Secondary used at 1/250 polyclonal Goat anti-rabbit IgG (H+L) conjugated to Alexa Fluor 568 (ab175471).Left image: DAPI staining nuclei (blue)Middle image: Hsp90 (red)Right image: Overlay
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Flow Cytometry - Anti-Hsp90 antibody (ab13495)Image courtesy of Dr Mahesh Shivananjappa by Abreview.ab13495 staining Hsp90 in Human platelet cells by Flow cytometry.
Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton-X-100 in 2% BSA for 15 minutes. Primary antibody used at a 1/250 dilution and incubated for 18 hours at 4°C. The secondary antibody used was an Alexa Fluor®488 conjugated chicken anti-rabbit IgG (H+L) at a 1/500 dilution.P : Permeabilized; US : Unstained, Red Peak; IgG RB : IgG Rabbit (Isotype Control) ab171870), Blue Peak; HSP90, Green peak.
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Western blot - Anti-Hsp90 antibody (ab13495)Image courtesy of Dr Mahesh Shivananjappa by Abreview.Anti-Hsp90 antibody (ab13495) at 1/1000 dilution + whole cell lysate prepared from human platelets treated with A23187 for 1 hour at 20 µg
Secondary
HRP conjugated goat anti-rabbit polyclonal at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 83.2 (beta) , 84.5 (alpha) kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Additional bands at: 36 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
Protocols
References
This product has been referenced in:
- Cho S et al. Progerin phosphorylation in interphase is lower and less mechanosensitive than lamin-A,C in iPS-derived mesenchymal stem cells. Nucleus 9:230-245 (2018). Read more (PubMed: 29619860) »
- An T et al. Comparative analysis of proteomes between diabetic and normal human sperm: Insights into the effects of diabetes on male reproduction based on the regulation of mitochondria-related proteins. Mol Reprod Dev 85:7-16 (2018). Read more (PubMed: 29149484) »
