Anti-Hsp90 antibody (ab13495)

Reviews (14)Q&A (7)References (30)

Overview

  • Product name

    Anti-Hsp90 antibody
    See all Hsp90 primary antibodies

  • Description

    Rabbit polyclonal to Hsp90

  • Host species

    Rabbit

  • Specificity

    Detects 90kD proteins corresponding to the molecular mass of hsp90aß.

  • Tested applications

    Suitable for: ELISA, IP, IHC-P, IHC-Fr, ICC/IF, ICC, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human, Xenopus laevis

  • Immunogen

    Human Hsp90.

  • Positive control

    • RAW 264.7 whole cell lysate (ab7187) can be used as a positive control in WB. HeLa Cell Lysate (Heat Shocked). Hsp90 Protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab13495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
Flow Cyt 1/250.

(see Abreview).

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/20000 - 1/40000. Detects a band of approximately 90 kDa (predicted molecular weight: 83.2 (beta) , 84.5 (alpha) kDa).

Target

  • Function

    Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.

  • Sequence similarities

    Belongs to the heat shock protein 90 family.

  • Domain

    The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins like the co-chaperone STUB1.

  • Post-translational
    modifications

    ISGylated.
    S-nitrosylated; negatively regulates the ATPase activity and the activation of eNOS by HSP90AA1.

  • Cellular localization

    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Heat shock 86 kDa antibody
    • Heat shock protein 90kDa alpha cytosolic class A member 1 antibody
    • Heat shock protein 90kDa alpha cytosolic class B member 1 antibody
    • Heat shock protein HSP 90 alpha antibody
    • Heat shock protein HSP 90 beta antibody
    • Heat shock protein HSP 90-alpha antibody
    • HS90A_HUMAN antibody
    • HSP 84 antibody
    • HSP 86 antibody
    • Hsp 90 antibody
    • HSP86 antibody
    • HSP90A antibody
    • HSP90AA1 antibody
    • HSP90AB1 antibody
    • HSP90B antibody
    • HSPC1 antibody
    • HSPC2 antibody
    • HSPCAL1 antibody
    • HSPCAL4 antibody
    • Renal carcinoma antigen NY-REN-38 antibody
    see all

Images

  • Western blot - Anti-Hsp90 antibody (ab13495)
    Western blot - Anti-Hsp90 antibody (ab13495)
    All lanes : Anti-Hsp90 antibody (ab13495) at 1/10000 dilution (2 hours at room temperature)

    Lane 1 : A431 with BSA for 30 minutes at room temperature
    Lane 2 : A549 with BSA for 30 minutes at room temperature
    Lane 3 : HCT116 with BSA for 30 minutes at room temperature
    Lane 4 : Hela with BSA for 30 minutes at room temperature
    Lane 5 : HEK293 with BSA for 30 minutes at room temperature
    Lane 6 : HepG2 with BSA for 30 minutes at room temperature
    Lane 7 : HL-60 with BSA for 30 minutes at room temperature
    Lane 8 : HUVEC with BSA for 30 minutes at room temperature
    Lane 9 : Jurkat with BSA for 30 minutes at room temperature
    Lane 10 : MCF7 with BSA for 30 minutes at room temperature
    Lane 11 : PC3 with BSA for 30 minutes at room temperature
    Lane 12 : T98G with BSA for 30 minutes at room temperature

    Lysates/proteins at 2 µg per lane.

    Blocking peptides at 1.5 % per lane.

    Secondary
    All lanes : HRP Donkey anti-rabbit IgG, 1 hour at room temperature

    Predicted band size: 83.2 (beta) , 84.5 (alpha) kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 antibody (ab13495)
    Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 antibody (ab13495)Image courtesy of Dr Mahesh Shivananjappa by Abreview.

    ab13495 staining Hsp90 in murine RAW 264 (ab7187).7 cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton-X100 in 2% BSA for 15 minutes, blocked with 2% BSA for 1 hour at 4°C and then incubated with ab13495 at a 1/150 dilution. The secondary used was an Alexa-Fluor 488 conjugated chicken anti-rabbit IgG (H+L) used at a 1/500 dilution.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 antibody (ab13495)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 antibody (ab13495)

    ab13495 staining Hsp90 in Mouse backskin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with Bouin's fixative solution. Samples were incubated with primary antibody (1/100) for 1 hour at room temperature. A FITC-conjugated Goat anti-rabbit IgG polyclonal (1/50) was used as the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 antibody (ab13495)
    Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 antibody (ab13495)This image was kindly supplied by Dr Mahesh Shivananjappa by Abreview

    ab13495 at a 1/100 dilution staining Hsp90 in human PMN cells by Immunocytochemistry/ Immunofluorescence incubated for 4 hours at 37°C. PFA fixed. Blocked using 2% BSA for 1 hour at 22°C. Secondary used at 1/250 polyclonal Goat anti-rabbit IgG (H+L) conjugated to Alexa Fluor 568 (ab175471).Left image: DAPI staining nuclei (blue)Middle image: Hsp90 (red)Right image: Overlay

    See Abreview

  • Flow Cytometry - Anti-Hsp90 antibody (ab13495)
    Flow Cytometry - Anti-Hsp90 antibody (ab13495)Image courtesy of Dr Mahesh Shivananjappa by Abreview.

    ab13495 staining Hsp90 in Human platelet cells by Flow cytometry.
    Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton-X-100 in 2% BSA for 15 minutes. Primary antibody used at a 1/250 dilution and incubated for 18 hours at 4°C. The secondary antibody used was an Alexa Fluor®488 conjugated chicken anti-rabbit IgG (H+L) at a 1/500 dilution.

    P : Permeabilized; US : Unstained, Red Peak; IgG RB : IgG Rabbit (Isotype Control) ab171870), Blue Peak; HSP90, Green peak.

    See Abreview

  • Western blot - Anti-Hsp90 antibody (ab13495)
    Western blot - Anti-Hsp90 antibody (ab13495)Image courtesy of Dr Mahesh Shivananjappa by Abreview.
    Anti-Hsp90 antibody (ab13495) at 1/1000 dilution + whole cell lysate prepared from human platelets treated with A23187 for 1 hour at 20 µg

    Secondary
    HRP conjugated goat anti-rabbit polyclonal at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 83.2 (beta) , 84.5 (alpha) kDa
    Observed band size: 90 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 36 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 5 minutes

    See Abreview

References

Publishing research using ab13495? Please let us know so that we can cite the reference in this datasheet.

ab13495 has been referenced in 30 publications.

  • Cho S  et al. Progerin phosphorylation in interphase is lower and less mechanosensitive than lamin-A,C in iPS-derived mesenchymal stem cells. Nucleus 9:230-245 (2018). PubMed: 29619860
  • An T  et al. Comparative analysis of proteomes between diabetic and normal human sperm: Insights into the effects of diabetes on male reproduction based on the regulation of mitochondria-related proteins. Mol Reprod Dev 85:7-16 (2018). PubMed: 29149484
  • Zheng H  et al. Is co-expression of USP22 and HSP90 more effective in predicting prognosis of gastric cancer? Pathol Res Pract N/A:N/A (2018). PubMed: 30598339
  • Zhang MQ  et al. Differential Effects of Autophagy-Related 10 Protein on HCV Replication and Autophagy Flux Are Mediated by Its Cysteine44 and Cysteine135. Front Immunol 9:2176 (2018). PubMed: 30319633
  • Zhang M  et al. Jujuboside A promotes Aß clearance and ameliorates cognitive deficiency in Alzheimer's disease through activating Axl/HSP90/PPAR? pathway. Theranostics 8:4262-4278 (2018). PubMed: 30128052
View all Publications for this product

Customer reviews and Q&As

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1-10 of 21 Abreviews or Q&A

Western blot abreview for Anti-Hsp90 antibody

 Good
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Spinal Cord)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
50 µg
Specification
Spinal Cord
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Jul 02 2015

Western blot abreview for Anti-Hsp90 antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (8% Gel)
Sample
Mouse Tissue lysate - whole (Skeletal muscle)
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Dec 11 2014

Western blot abreview for Anti-Hsp90 antibody

 Good
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (8% Gel)
Sample
Rat Tissue lysate - whole (Skeletal muscle)
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Dec 04 2014

Western blot abreview for Anti-Hsp90 antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (4-20% gradient gel)
Sample
Rat Tissue lysate - whole (rat brain celles)
Specification
rat brain celles
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 4°C
Read More

Abcam user community

Verified customer

Submitted Oct 10 2014

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Hsp90 antibody

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Antigen retrieval step
None
Sample
Human Tissue sections (skin)
Specification
skin
Permeabilization
No
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Jun 10 2014

Western blot abreview for Anti-Hsp90 antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Non-reduced Denaturing (7.5)
Sample
Human Cell lysate - whole cell (Glioblastoma cell lines)
Specification
Glioblastoma cell lines
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Read More

Mr. Achilleas Mitrakas

Verified customer

Submitted Apr 28 2014

Immunocytochemistry/ Immunofluorescence abreview for Anti-Hsp90 antibody

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Human Cell (U87MG Human brain glioblastoma)
Specification
U87MG Human brain glioblastoma
Permeabilization
Yes - 0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
Fixative
Paraformaldehyde
Read More

Dr. Dimitra Kalamida

Verified customer

Submitted Nov 18 2013

Question

Thank you for your kindly reply.

Our customer raises inquiries about our products: Anti-Hsp90 antibody (ab13495) and Anti-Hsp70 antibody [5A5] (ab2787).

She would like to test in bufo bankorensis / WB. She didn’t have the protein sequences for Hsp90 and Hsp70 from bufo bankorensis.

She have the HSP90 and Hsp70 gene of bufo bankorensis in text format.

Please find the HSP90 and Hsp70 gene of bufo bankorensis in the attachment.

Would you please help this customer to create a discount code, the information of this customer as follow:

xxxxxxxxxxxxxxxxx

Thank you very much.

Best regards,

Read More
Answer

Thank you for providing those details.

The sequences provided are only truncations of the proteins and I have therefore not been able to compare them to the immunogens used to raise the antibodies ab2787 and ab13495. I cannot therefore ascertain how likely it is that the antibodies will cross react with your customers bufo bankorensis samples.

I have however performed a blast of the two immunogens used and they each resulted with a few sequences of toad derived proteins matching with higher than 85% homology. It is therefore possible that the antibodies will cross react. I am therefore willing to provide the testing discount codes as a gesture of goodwill. The codes are as follows:

ab2781, Discount code: xxxxx expiration date 26th December 2012
ab13495, Discount code: xxxxx expiration date 26th December 2012

I hope this has been of help. If you have any further questions, please do not hesitate to contact us again.

Read More

Question

Dear xxxxx,

Sorry to disturb you. Our customer raises inquiries about our products: Anti-Hsp90 antibody (ab13495) and Anti-Hsp70 antibody [5A5] (ab2787).

She would like to test in bufo bankorensis / WB. She didn’t have the protein sequences for Hsp90 and Hsp70 from bufo bankorensis.

She have the HSP90 and Hsp70 gene of bufo bankorensis. Please find the HSP90 and Hsp70 gene of bufo bankorensis in the attachment.

Would you please help this customer to create a discount code, the information of this customer as follow:

xxxxxx
Thank you very much.

Best regards,

Read More
Answer

Thank you very much for providing those sequences.

In order to help the customer, could you please provide me the sequence in text format (i.e. in the body of the email, or as a word document). This will allow me to copy and paste the sequence to be able to find the protein sequence and align this to the immunogens used to raise the Hsp90 and Hsp70. This will allow me to advise the customer as to whether the antibodies are likely to be able tocross-react withthe proteins of interest or not.

I look forward to receiving your reply.

Read More

Question

Dear Sir/ Madam,

Sorry to disturb you. Our customer raises inquiries about our products: Anti-Hsp90 antibody (ab13495) and Anti-Hsp70 antibody [5A5] (ab2787).

She would like to test in bufo bankorensis / WB, would you please help this customer to create a discount code, the information of this customer as follow:

Thank you very much.

Best regards,

Read More
Answer

Thank you for contacting us.

I have been unable to find a sequence for Hsp90 and Hsp70 in bufo bankorensis, I am therefore not able to say if the antibodies are likely to be able to detect the proteins from these samples. Would you be able to ask the customer is they have the protein sequences for Hsp90 and Hsp70from bufo bankorensis and I will check this for them. I can then let you know if the customer would be eligiblefor the testing discount scheme or not.

Many thanks in advance for your assistance in this matter.

Read More

1-10 of 21 Abreviews or Q&A

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