Anti-Hsp90 antibody [AC88] (ab13492)

Mouse monoclonal Hsp90 antibody [AC88]. Validated in WB, IP, IHC, ICC, Flow Cyt, ICC/IF and tested in Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig and more.

Overview

  • Product name
    Anti-Hsp90 antibody [AC88]
    See all Hsp90 primary antibodies
  • Description
    Mouse monoclonal [AC88] to Hsp90
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-Fr, IHC-P, ICC/IF, WB, IP, Flow Cyt, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig, Caenorhabditis elegans, Carp, Fish, Monkey, African green monkey, Rainbow trout
  • Immunogen

    Full length native protein (purified) corresponding to Hsp90. from Achlya ambisexualis (water mold).

  • Epitope
    The epitope of this antibody has been mapped to amino acid residues 604-697 of the human Hsp90 sequence.
  • Positive control
    • ICC/IF: Panc-1 and HepG2 cells. WB: HeLa Cell Lysate (Heat Shocked), Hsp90 Protein; HepG2 cell lysate; PC12 cell lysate; NIH/3T3 cell lysate; Rat2 cell lysate. IHC-P: Human spleen and testis tissue. IHC-fr: Human stomach tissue.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 22nd May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    AC88
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab13492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 90 kDa.

84.7 (alpha) , 83.2  (beta)

IP Use at an assay dependent concentration.

When used in immunoprecipitation, this antibody does not appear to react well with Hsp90 that is bound to steroid receptors or to pp60v-src of Rouse sarcoma virus.

Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC Use at an assay dependent concentration.

Target

Images

  • ab13492 staining Hsp90 (red) and another antibody to C2GnT-M (Golgi enzyme, greeen) in Panc-1 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with PFA and blocked with 1% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/50 1% Donkey serum in PBST) for 1 hour at 22°C. An undiluted DyLight® 594-conjugated Donkey anti-mouse IgG polyclonal was used as the secondary antibody.

    This image was generated using the ascites version of the product. 

    See Abreview

  • All lanes : Anti-Hsp90 antibody [AC88] (ab13492) at 1/1000 dilution

    Lane 2 : HSP90 cell lysate
    Lane 3 : HSP90ß cell lysate
    Lane 4 : HSP90a cell lysate
    Lane 5 : HeLa (heat shocked) cell lysate
    Lane 6 : NIH/3T3 (Heat shocked) cell lysate
    Lanes 7-8 : PC-12 (Heat shocked) cell lysate


    This image was generated using the ascites version of the product.

  • Anti-Hsp90 antibody [AC88] (ab13492) at 1/1000 dilution + Human fibroblast whole cell lysate at 40 µg

    Secondary
    HRP-conjugated goat anti-mouse polyclonal IgG at 1/2000 dilution

    Developed using the ECL technique.

    Exposure time: 20 seconds


    Blocked with 5% Milk for 2 hours at 22°C

    This image was generated using the ascites version of the product.

    See Abreview

  • Frozen sections of human stomach tissue stained for Hsp90 using ab13492 at 1/100 dilution in immunohistochemical analysis.

    This image was generated using the ascites version of the product.

  • ab13492 staining Hsp90 in Human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a TRIS-EDTA Buffer. Samples were incubated with primary antibody (1/500) for 30 minutes at 20°C. A HRP-conjugated Goat anti-rabbit/mouse IgG polyclonal was used as the secondary antibody.

    This image was generated using the ascites version of the product.

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab13492 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum (ab7481) / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13492, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    This image was generated using the ascites version of the product.

  • ab13492 staining Hsp90 in Human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (10 ug/ml).

    This image was generated using the ascites version of the product.

  • All lanes : Anti-Hsp90 antibody [AC88] (ab13492) at 1/1000 dilution

    Lane 1 : Hsp90 native human protein
    Lane 2 : Hsp90 beta reombinant human protein
    Lane 3 : Hsp90 alpha reombinant human protein
    Lane 4 : Cell lysates prepared from heat shocked Hela cells
    Lane 5 : Cell lysates prepared from heat shocked 3T3 cells
    Lane 6 : Cell lysates prepared from heat shocked PC-12 cells
    Lane 7 : Cell lysates prepared from heat shocked CHO-K1 cells
    Lane 8 : Cell lysates prepared from heat shocked Rat-2 cells


    This image was generated using the ascites version of the product.

  • ICC/IF image of ab13492 stained HepG2 cells (ab7900). The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13492, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-mouse IgG - H&L, pre-adsorbed (ab96879) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    This image was generated using the ascites version of the product.

  • ab13492 Immunoprecipitate OF hSP 90 in human AGS whole cell lysate. 200µg of cell lysate was incubated with primary antibody (1/250 in RIPA buffer) and matrix (Protein A/G) for 16 hours at 4°C.
    For western blotting anti-mouse HRP (ab6728) (1/1000) was used.

    This image was generated using the ascites version of the product.

    See Abreview

References

This product has been referenced in:
  • Dong L  et al. HSP90 interacts with HMGCR and promotes the progression of hepatocellular carcinoma. Mol Med Rep 19:524-532 (2019). Read more (PubMed: 30483734) »
  • Eckersley-Maslin M  et al. Dppa2 and Dppa4 directly regulate the Dux-driven zygotic transcriptional program. Genes Dev 33:194-208 (2019). Read more (PubMed: 30692203) »
See all 51 Publications for this product

Customer reviews and Q&As

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1-10 of 26 Abreviews

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X-100
Specification
HeLa
Fixative
Paraformaldehyde

Dr. Kirk Mcmanus

Verified customer

Submitted Aug 10 2018

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (murine proerythroblast)
Permeabilization
Yes - Tween-20
Specification
murine proerythroblast
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Acetone

Abcam user community

Verified customer

Submitted Jan 12 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Normal kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6.0 Citric Acid
Permeabilization
Yes - Tween-20
Specification
Normal kidney
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jan 12 2018

Application
Immunocytochemistry
Sample
Human Cultured Cells (Human Renal Epithelial Cells)
Permeabilization
Yes - 0.1% Triton
Specification
Human Renal Epithelial Cells
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Formaldehyde

Neda Ghousifam

Verified customer

Submitted May 31 2017

Application
Western blot
Sample
Hamster Cell lysate - whole cell (CHO cells)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
20 µg
Treatment
Infected with virus
Specification
CHO cells
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Aug 24 2016

Application
Immunohistochemistry (Frozen sections)
Blocking step
0.25%TritonX100, 0.2%Gelatine as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
Sample
Mouse Tissue sections (mouse embryonic spinal cord E11)
Specification
mouse embryonic spinal cord E11
Permeabilization
Yes - 0.25%TritonX100
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 01 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0
Sample
Human Tissue sections (Testis)
Specification
Testis
Permeabilization
Yes - Wash Buffer from Dako with Tween
Fixative
Paraformaldehyde

Mr. Rudolf Jung

Verified customer

Submitted Mar 31 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Sample
Human Cell (Panc-1)
Specification
Panc-1
Permeabilization
No
Fixative
Paraformaldehyde

Dr. Armen Petrosyan

Verified customer

Submitted Nov 19 2013

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Sample
Human Cell lysate - whole cell (Panc-1 cell lysate)
Specification
Panc-1 cell lysate
Treatment
1-control 2- drug KNK437 35 mcm for 1h 3 - drug Brefeldin A 30 mcm for 30 min
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Armen Petrosyan

Verified customer

Submitted Nov 18 2013

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Cow Cell lysate - whole cell (oviductal epithelial cells)
Specification
oviductal epithelial cells
Blocking step
PVDF Blocking Reagent (TOYOBO) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 06 2013

1-10 of 26 Abreviews

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