Recombinant
RabMAb

Recombinant Anti-Hsp90 antibody [EPR3953] - BSA and Azide free (ab239960)

Overview

  • Product name

    Anti-Hsp90 antibody [EPR3953] - BSA and Azide free
    See all Hsp90 primary antibodies
  • Description

    Rabbit monoclonal [EPR3953] to Hsp90 - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Hsp90. The exact sequence is proprietary.

  • Positive control

    • IHC-P: Human gastric adenocarcinoma tissue, Human lung carcinoma and Human kidney tissue
  • General notes

    Ab239960 is the carrier-free version of ab109248. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab239960 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab239960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

WB Use at an assay dependent concentration. Detects a band of approximately 90 kDa (predicted molecular weight: 85 kDa).
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.
    • Sequence similarities

      Belongs to the heat shock protein 90 family.
    • Domain

      The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins like the co-chaperone STUB1.
    • Post-translational
      modifications

      ISGylated.
      S-nitrosylated; negatively regulates the ATPase activity and the activation of eNOS by HSP90AA1.
    • Cellular localization

      Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links

    • Alternative names

      • Heat shock 86 kDa antibody
      • Heat shock protein 90kDa alpha cytosolic class A member 1 antibody
      • Heat shock protein 90kDa alpha cytosolic class B member 1 antibody
      • Heat shock protein HSP 90 alpha antibody
      • Heat shock protein HSP 90 beta antibody
      • Heat shock protein HSP 90-alpha antibody
      • HS90A_HUMAN antibody
      • HSP 84 antibody
      • HSP 86 antibody
      • Hsp 90 antibody
      • HSP86 antibody
      • HSP90A antibody
      • HSP90AA1 antibody
      • HSP90AB1 antibody
      • HSP90B antibody
      • HSPC1 antibody
      • HSPC2 antibody
      • HSPCAL1 antibody
      • HSPCAL4 antibody
      • Renal carcinoma antigen NY-REN-38 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling Hsp90 with purified ab109248 at 1:50 dilution (19.42 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • ab109248, at 1/100, staining Hsp90 in paraffin-embedded Human gastric adenocarcinoma tissue by Immunohistochemistry.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109248).

    • ab109248, at 1/100, staining Hsp90 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109248).

    References

    ab239960 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab239960.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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