Anti-Hsp90 antibody [Hyb-K41220A] (ab241995)
Key features and details
- Mouse monoclonal [Hyb-K41220A] to Hsp90
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2a
Overview
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Product name
Anti-Hsp90 antibody [Hyb-K41220A]
See all Hsp90 primary antibodies -
Description
Mouse monoclonal [Hyb-K41220A] to Hsp90 -
Host species
Mouse -
Specificity
Will detect both alpha (inducible) and beta (constitutively-expressed) forms.
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Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human Hsp90. Specificity mapped to amino acids 291-304.
Database link: P07900 -
Epitope
Specificity mapped to amino acids 291-304. -
Positive control
- WB: A431, A594, HCT 116, HeLa, HepG2, HL-60, HUVEC, Jurkat, MCF7, PC-3 and T98G cell lysates; rat brain tissue lysate. ICC/IF: HeLa cells. IHC-P: Mouse backskin.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
Hyb-K41220A -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab241995 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 85 kDa.
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ICC/IF |
1/100.
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IHC-P |
1/100.
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Notes |
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WB
1/1000. Predicted molecular weight: 85 kDa. |
ICC/IF
1/100. |
IHC-P
1/100. |
Target
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Function
Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. -
Sequence similarities
Belongs to the heat shock protein 90 family. -
Domain
The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins like the co-chaperone STUB1. -
Post-translational
modificationsISGylated.
S-nitrosylated; negatively regulates the ATPase activity and the activation of eNOS by HSP90AA1. -
Cellular localization
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 3320 Human
- Entrez Gene: 3326 Human
- Entrez Gene: 15516 Mouse
- Entrez Gene: 15519 Mouse
- Entrez Gene: 299331 Rat
- Entrez Gene: 301252 Rat
- Omim: 140571 Human
- Omim: 140572 Human
see all -
Alternative names
- Heat shock 86 kDa antibody
- Heat shock protein 90kDa alpha cytosolic class A member 1 antibody
- Heat shock protein 90kDa alpha cytosolic class B member 1 antibody
see all
Images
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Bouin's fixed, paraffin-embedded mouse back skin tissue stained for Hsp90 using ab241995 at 1/100 dilution for 1 hr at RT in immunohistochemical analysis.
Secondary antibody is FITC Goat Anti-Mouse at 1/50 dilution for 1 hr at RT.
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All lanes : Anti-Hsp90 antibody [Hyb-K41220A] (ab241995) at 1/1000 dilution
Lane 1 : A431 (Human epidermoid carcinoma cell line) cell lysate
Lane 2 : A549 (human lung carcinoma cell line) cell lysate
Lane 3 : HCT 116 (human colorectal carcinoma cell line) cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 5 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 6 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 7 : HL-60 (human promyelocytic leukemia cell line) cell lysate
Lane 8 : HUVEC (human umbilical vein endothelial cell line) cell lysate
Lane 9 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 10 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 11 : PC-3 (Human prostate adenocarcinoma cell line) cell lysate
Lane 12 : T98G (Human glioblastoma multiforme) cell lysate
Lane 13 : Rat brain tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
Lanes 1-5 & 7-13 : Sheep Anti-Mouse IgG: HRP
Predicted band size: 85 kDaPrimary incubation for 2 hours at RT.
Secondary incubation for 1 hour at RT.
Block: 1.5% BSA for 30 minutes at RT.
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2% formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Hsp90 (green, B) using ab241995 at 1/100 dilution for 12 hours at 4°C in ICC/IF. Secondary antibody is Goat anti-Mouse FITC at 1/200 dilution. The nuclear counterstain is DAPI (blue, A). (C) is a composite of (A) and (B).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab241995 has not yet been referenced specifically in any publications.