Key features and details
- Mouse monoclonal [Hyb-K41220A] to Hsp90
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG2a
Product nameAnti-Hsp90 antibody [Hyb-K41220A]
See all Hsp90 primary antibodies
DescriptionMouse monoclonal [Hyb-K41220A] to Hsp90
Will detect both alpha (inducible) and beta (constitutively-expressed) forms.
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Rat, Human
Recombinant fragment corresponding to Human Hsp90. Specificity mapped to amino acids 291-304.
Database link: P07900
EpitopeSpecificity mapped to amino acids 291-304.
- WB: A431, A594, HCT 116, HeLa, HepG2, HL-60, HUVEC, Jurkat, MCF7, PC-3 and T98G cell lysates; rat brain tissue lysate. ICC/IF: HeLa cells. IHC-P: Mouse backskin.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab241995 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 85 kDa.|
FunctionMolecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.
Sequence similaritiesBelongs to the heat shock protein 90 family.
DomainThe TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins like the co-chaperone STUB1.
S-nitrosylated; negatively regulates the ATPase activity and the activation of eNOS by HSP90AA1.
Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- Heat shock 86 kDa antibody
- Heat shock protein 90kDa alpha cytosolic class A member 1 antibody
- Heat shock protein 90kDa alpha cytosolic class B member 1 antibody
Bouin's fixed, paraffin-embedded mouse back skin tissue stained for Hsp90 using ab241995 at 1/100 dilution for 1 hr at RT in immunohistochemical analysis.
Secondary antibody is FITC Goat Anti-Mouse at 1/50 dilution for 1 hr at RT.
All lanes : Anti-Hsp90 antibody [Hyb-K41220A] (ab241995) at 1/1000 dilution
Lane 1 : A431 (Human epidermoid carcinoma cell line) cell lysate
Lane 2 : A549 (human lung carcinoma cell line) cell lysate
Lane 3 : HCT 116 (human colorectal carcinoma cell line) cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 5 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 6 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 7 : HL-60 (human promyelocytic leukemia cell line) cell lysate
Lane 8 : HUVEC (human umbilical vein endothelial cell line) cell lysate
Lane 9 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 10 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 11 : PC-3 (Human prostate adenocarcinoma cell line) cell lysate
Lane 12 : T98G (Human glioblastoma multiforme) cell lysate
Lane 13 : Rat brain tissue lysate
Lysates/proteins at 15 µg per lane.
Lanes 1-5 & 7-13 : Sheep Anti-Mouse IgG: HRP
Predicted band size: 85 kDa
Primary incubation for 2 hours at RT.
Secondary incubation for 1 hour at RT.
Block: 1.5% BSA for 30 minutes at RT.
2% formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Hsp90 (green, B) using ab241995 at 1/100 dilution for 12 hours at 4°C in ICC/IF. Secondary antibody is Goat anti-Mouse FITC at 1/200 dilution. The nuclear counterstain is DAPI (blue, A). (C) is a composite of (A) and (B).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab241995 has not yet been referenced specifically in any publications.