Anti-Hsp90 beta antibody [H90-10] (ab53497)

Abreviews (1)Q&A (2)Specific References (5)

Overview

  • Product name
    Anti-Hsp90 beta antibody [H90-10]
    See all Hsp90 beta primary antibodies
  • Description
    Mouse monoclonal [H90-10] to Hsp90 beta
  • Host species
    Mouse
  • Tested applications
    Suitable for: Flow Cyt, ELISA, IHC-Fr, IHC-P, ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Chicken, Human
  • Immunogen

    Recombinant full length Hsp90 beta protein (Human)

  • Positive control
    • WB: Recombinant human Hsp90 beta protein. IHC-P: Human placenta, backskin and colon carcinoma tissue; mouse colon tissue. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    H90-10
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53497 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.5µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IHC-P 1/100.
ICC/IF Use at an assay dependent concentration.
WB 1/2500. Predicted molecular weight: 83 kDa.
IP Use at an assay dependent concentration.

Target

  • Function
    Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.
  • Sequence similarities
    Belongs to the heat shock protein 90 family.
  • Domain
    The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins.
  • Post-translational
    modifications
    Ubiquitinated in the presence of STUB1-UBE2D1 complex (in vitro).
    ISGylated.
    S-nitrosylated; negatively regulates the ATPase activity.
  • Cellular localization
    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • 90 kda heat shock protein beta HSP90 beta antibody
    • D6S182 antibody
    • FLJ26984 antibody
    • Heat shock 84 kDa antibody
    • Heat shock 90kD protein 1, beta antibody
    • Heat shock 90kDa protein 1 beta antibody
    • Heat shock protein 90 alpha family class B member 1 antibody
    • Heat shock protein 90 kDa antibody
    • Heat shock protein 90kDa alpha (cytosolic) class B member 1 antibody
    • Heat shock protein 90kDa alpha family class B member 1 antibody
    • Heat shock protein beta antibody
    • Heat shock protein HSP 90 beta antibody
    • Heat shock protein HSP 90-beta antibody
    • HS90B_HUMAN antibody
    • HSP 84 antibody
    • HSP 90 antibody
    • HSP 90 b antibody
    • HSP 90b antibody
    • HSP84 antibody
    • HSP90 BETA antibody
    • hsp90ab1 antibody
    • HSP90B antibody
    • HSPC2 antibody
    • HSPCB antibody
    see all

Images

  • Western blot - Anti-Hsp90 beta antibody [H90-10] (ab53497)
    Western blot - Anti-Hsp90 beta antibody [H90-10] (ab53497)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [H90-10] (ab53497)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [H90-10] (ab53497)
    Ab53497 staining Human normal placenta. Staining is localized to cytoplasmic compartment.
    Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 beta antibody [H90-10] (ab53497)
    Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 beta antibody [H90-10] (ab53497)
    ICC/IF image of ab53497 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53497, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [H90-10] (ab53497)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [H90-10] (ab53497)

    Paraffin-embedded mouse backskin (epidermis) tissue fixed with Bouin's fixative, stained for Hsp90 beta using ab53497 at 1/100 dilution in immunohistochemical analysis. Primary antibody was incubated for 1 hour at room temperature. Secondary antibody was a FITC-conjugated goat anti-mouse (green) at 1/50 dilution ncubated for 1 hour at room temperature.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [H90-10] (ab53497)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [H90-10] (ab53497)

    Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for Hsp90 beta using ab53497 at 1/10000 in immunohistochemical analysis. Primary antibody was incubated for 12 hours at 4°C. Secondary antibody was an Alexa Fluor® 555 goat anti-mouse (red) at 1/5000 dilution incubated for 1 hour at room temperature.

    40x magnification.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [H90-10] (ab53497)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [H90-10] (ab53497)

    ab53497 at 100,000 dilution staining Hsp90 in human colon cancer tissue section by immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). A antibody amplifier™ system was used for staining. A HRP-conjugated secondary antibody was used at 1/10 dilution

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [H90-10] (ab53497)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [H90-10] (ab53497)

    ab53497 at 100,000 dilution staining Hsp90 beta in mouse colon tissue section by immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). A antibody amplifier™ system was used for staining. An Alexa Fluor®  568 conjugated secondary antibody was used at 1/10 dilution.

  • Western blot - Anti-Hsp90 beta antibody [H90-10] (ab53497)
    Western blot - Anti-Hsp90 beta antibody [H90-10] (ab53497)
    All lanes : Anti-Hsp90 beta antibody [H90-10] (ab53497)

    Lane 1 : Hsp90 beta protein
    Lane 2 : Hsp90 alpha protein

    Lysates/proteins at 2 µg per lane.

    Predicted band size: 83 kDa

  • Flow Cytometry - Anti-Hsp90 beta antibody [H90-10] (ab53497)
    Flow Cytometry - Anti-Hsp90 beta antibody [H90-10] (ab53497)
    Overlay histogram showing HeLa cells stained with ab53497 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53497, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References

This product has been referenced in:
  • Sha L  et al. Pharmacologic inhibition of Hsp90 to prevent GLT-1 degradation as an effective therapy for epilepsy. J Exp Med 214:547-563 (2017). Read more (PubMed: 28028152) »
  • Carpp LN  et al. Quantitative proteomic analysis of host-virus interactions reveals a role for Golgi brefeldin A resistance factor 1 (GBF1) in dengue infection. Mol Cell Proteomics 13:2836-54 (2014). Read more (PubMed: 24855065) »

See all 5 Publications for this product

Publishing research using ab53497? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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3 reviews or Q&A

10
Votes: Highest First

Thank your for your kindness,and I wish to have my antibodies refunded. Could you please tell 51AB company to contact me before refunding me?

Thank you for your reply. I will forward your request to 51AB, who will be in contact with you to process your refund request. I hope this information is helpful.  Please do not hesitate to contact us if you have any additional questions.

To whom it may concern, I bought two primary antibodies ab79849 and ab53497,which have been used in CoIP,but the results are confusing. I intend to verify the combination between HSP90α and HBP21,and that between HSP90β and HBP21using protein A+G...

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Than you for your inquiry and I am sorry to hear that you are experiencing some trouble with ab53497 and ab79849. Are you running any isotype controls with these experiments? I just want to make sure that the pull down with the HBP21 antibody i...

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Western blot abreview for Anti-Hsp90 beta antibody [H90-10]

 Excellent
Abreviews
Application
Western blot
Sample
Fish Tissue lysate - whole (Heart, brain, liver, white muscle, red muscle)
Loading amount
60 µg
Specification
Heart, brain, liver, white muscle, red muscle
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (7.5)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
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Dr. Katja Anttila

Verified customer

Submitted Mar 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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