Key features and details
- Rabbit polyclonal to Hsp90 beta (phospho S226)
- Suitable for: WB, ELISA, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Hsp90 beta (phospho S226) antibody
See all Hsp90 beta primary antibodies
DescriptionRabbit polyclonal to Hsp90 beta (phospho S226)
SpecificityDetects Hsp90 beta only when phosphorylated at serine 226.
Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic phosphopeptide derived from human Hsp90 beta around the phosphorylation site of serine 226 (E-I-SP-D-D).
- NIH/3T3 cells.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab63562 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).|
|ICC/IF||1/500 - 1/1000.|
FunctionMolecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.
Sequence similaritiesBelongs to the heat shock protein 90 family.
DomainThe TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins.
modificationsUbiquitinated in the presence of STUB1-UBE2D1 complex (in vitro).
S-nitrosylated; negatively regulates the ATPase activity.
Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- 90 kda heat shock protein beta HSP90 beta antibody
- D6S182 antibody
- FLJ26984 antibody
All lanes : Anti-Hsp90 beta (phospho S226) antibody (ab63562) at 1/500 dilution
Lane 1 : extracts from A549 cells, treated
with serum (20%, 15mins)
Lane 2 : extracts from A549 cells, treated
with serum (20%, 15mins) with immunizing phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 83 kDa
Observed band size: 83 kDa
ab63562 at 1/500 dilution staining NIH/3T3 cells, without (left) and with (right) immunizing peptide.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab63562 has been referenced in 1 publication.
- Wang H et al. Hsp90a forms a stable complex at the cilium neck for the interaction of signalling molecules in IGF-1 receptor signalling. J Cell Sci 128:100-8 (2015). PubMed: 25359884