Product nameAnti-Hsp90 beta (phospho S254) antibody
See all Hsp90 beta primary antibodies
DescriptionRabbit polyclonal to Hsp90 beta (phospho S254)
SpecificityDetects endogenous levels of HSP90B only when phosphorylated at serine 254.
Tested applicationsSuitable for: ICC/IF, WB, ELISA, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthesized phosphopeptide derived from human HSP90B around the phosphorylation site of serine 254 (V-G-SP-D-E).
- Extracts from Hela cells treated with TNF-a, human breast carcinoma tissue. IF/ICC: HeLa cell line
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol, PBS
Without Mg+2 and Ca+2
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab51136 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||1/500 - 1/1000. Detects a band of approximately 87 kDa (predicted molecular weight: 83 kDa).|
|IHC-P||Use at an assay dependent concentration.|
FunctionMolecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.
Sequence similaritiesBelongs to the heat shock protein 90 family.
DomainThe TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins.
modificationsUbiquitinated in the presence of STUB1-UBE2D1 complex (in vitro).
S-nitrosylated; negatively regulates the ATPase activity.
Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- 90 kda heat shock protein beta HSP90 beta antibody
- D6S182 antibody
- FLJ26984 antibody
ICC/IF image of ab51136 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51136, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM
All lanes : Anti-Hsp90 beta (phospho S254) antibody (ab51136) at 1/500 dilution
Lane 1 : extracts from Hela cells treated with TNF-a with immunizing peptide
Lane 2 : extracts from Hela cells treated with TNF-a
Lysates/proteins at 0.02 µg/ml per lane.
Predicted band size: 83 kDa
Observed band size: 88 kDa why is the actual band size different from the predicted?
Ab51136 at 1/50 dilution staining human breast carcinoma tissue with and without blocking peptide; paraffin embedded.
ab51136 has not yet been referenced specifically in any publications.