Anti-Hsp90 beta (phospho S254) antibody (ab51136)
Key features and details
- Rabbit polyclonal to Hsp90 beta (phospho S254)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Hsp90 beta (phospho S254) antibody
See all Hsp90 beta primary antibodies -
Description
Rabbit polyclonal to Hsp90 beta (phospho S254) -
Host species
Rabbit -
Specificity
Detects endogenous levels of HSP90B only when phosphorylated at serine 254. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Hsp90 beta aa 200-300 (phospho S254).
Database link: P08238 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS
Without Mg+2 and Ca+2 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab51136 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/1000. Detects a band of approximately 87 kDa (predicted molecular weight: 83 kDa).
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IHC-P |
Use at an assay dependent concentration.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 87 kDa (predicted molecular weight: 83 kDa). |
IHC-P
Use at an assay dependent concentration. |
Target
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Function
Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. -
Sequence similarities
Belongs to the heat shock protein 90 family. -
Domain
The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins. -
Post-translational
modificationsUbiquitinated in the presence of STUB1-UBE2D1 complex (in vitro).
ISGylated.
S-nitrosylated; negatively regulates the ATPase activity. -
Cellular localization
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 3326 Human
- Omim: 140572 Human
- SwissProt: P08238 Human
- Unigene: 509736 Human
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Alternative names
- 90 kda heat shock protein beta HSP90 beta antibody
- D6S182 antibody
- FLJ26984 antibody
see all
Images
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All lanes : Anti-Hsp90 beta (phospho S254) antibody (ab51136) at 1/500 dilution
Lane 1 : extracts from Hela cells treated with TNF-a with immunizing peptide
Lane 2 : extracts from Hela cells treated with TNF-a
Lysates/proteins at 0.02 µg/ml per lane.
Predicted band size: 83 kDa
Observed band size: 88 kDa why is the actual band size different from the predicted? -
Ab51136 at 1/50 dilution staining human breast carcinoma tissue with and without blocking peptide; paraffin embedded.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab51136 has been referenced in 1 publication.
- Weeraphan C et al. Phosphoproteome Profiling of Isogenic Cancer Cell-Derived Exosome Reveals HSP90 as a Potential Marker for Human Cholangiocarcinoma. Proteomics 19:e1800159 (2019). PubMed: 31054213