Recombinant
RabMAb

Recombinant Anti-HSPA14 antibody [EPR4210] (ab108612)

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Overview

  • Product name

    Anti-HSPA14 antibody [EPR4210]
    See all HSPA14 primary antibodies

  • Description

    Rabbit monoclonal [EPR4210] to HSPA14

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human HSPA14 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • K562, COS1, Human fetal liver, and HeLa cell lysates. Human kidney tissue. HeLa cells.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR4210

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab108612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/10000. Detects a band of approximately 54 kDa (predicted molecular weight: 55 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Function

      Component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, binds to the nascent polypeptide chain, while DNAJC2 stimulates its ATPase activity.

    • Sequence similarities

      Belongs to the heat shock protein 70 family.

    • Cellular localization

      Cytoplasm > cytosol.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Heat shock 70 kDa protein 14 antibody
      • Heat shock 70kDa protein 14 antibody
      • Heat shock protein HSP60 antibody
      • Heat shock protein hsp70 related protein antibody
      • HSP60 antibody
      • HSP70 4 antibody
      • HSP70 like protein 1 antibody
      • HSP70-like protein 1 antibody
      • HSP70L1 antibody
      • HSP7E_HUMAN antibody
      • HSPA 14 antibody
      • HSPA14 antibody
      • MGC131990 antibody
      see all

    Images

    • Western blot - Anti-HSPA14 antibody [EPR4210] (ab108612)
      Western blot - Anti-HSPA14 antibody [EPR4210] (ab108612)
      All lanes : Anti-HSPA14 antibody [EPR4210] (ab108612) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : COS1 cell lysate
      Lane 3 : Human fetal liver lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 55 kDa
      Observed band size: 54 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA14 antibody [EPR4210] (ab108612)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA14 antibody [EPR4210] (ab108612)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue using ab108612 at 1/100.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-HSPA14 antibody [EPR4210] (ab108612)
      Immunocytochemistry/ Immunofluorescence - Anti-HSPA14 antibody [EPR4210] (ab108612)
      Immunofluorescent staining of HeLa cells using ab108612 at 1/100

    References

    This product has been referenced in:

    • Morgan SV  et al. Proteomic and cellular localisation studies suggest non-tight junction cytoplasmic and nuclear roles for occludin in astrocytes. Eur J Neurosci 47:1444-1456 (2018). Read more (PubMed: 29738614) »
    See 1 Publication for this product

    Publishing research using ab108612? Please let us know so that we can cite the reference in this datasheet.

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