Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4210] to HSPA14
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Product nameAnti-HSPA14 antibody [EPR4210]
See all HSPA14 primary antibodies
DescriptionRabbit monoclonal [EPR4210] to HSPA14
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human HSPA14 aa 50-150. The exact sequence is proprietary.
- K562, COS1, Human fetal liver, and HeLa cell lysates. Human kidney tissue. HeLa cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab108612 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/250.|
|WB||1/1000 - 1/10000. Detects a band of approximately 54 kDa (predicted molecular weight: 55 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionComponent of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, binds to the nascent polypeptide chain, while DNAJC2 stimulates its ATPase activity.
Sequence similaritiesBelongs to the heat shock protein 70 family.
Cellular localizationCytoplasm > cytosol.
- Information by UniProt
- Heat shock 70 kDa protein 14 antibody
- Heat shock 70kDa protein 14 antibody
- Heat shock protein HSP60 antibody
All lanes : Anti-HSPA14 antibody [EPR4210] (ab108612) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : COS-1 (African green monkey kidney fibroblast-like cell line) cell lysate
Lane 3 : Human fetal liver lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 55 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of paraffin-embedded Human kidney tissue using ab108612 at 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunofluorescent staining of HeLa cells using ab108612 at 1/100
ab108612 has been referenced in 1 publication.
- Morgan SV et al. Proteomic and cellular localisation studies suggest non-tight junction cytoplasmic and nuclear roles for occludin in astrocytes. Eur J Neurosci 47:1444-1456 (2018). PubMed: 29738614