Product nameAnti-HSPA14 antibody [EPR4210]
See all HSPA14 primary antibodies
DescriptionRabbit monoclonal [EPR4210] to HSPA14
Tested applicationsSuitable for: ICC/IF, WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human HSPA14 aa 50-150. The exact sequence is proprietary.
- K562, COS1, Human fetal liver, and HeLa cell lysates. Human kidney tissue. HeLa cells.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab108612 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/250.|
|WB||1/1000 - 1/10000. Detects a band of approximately 54 kDa (predicted molecular weight: 55 kDa).|
|IP||1/10 - 1/100.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionComponent of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, binds to the nascent polypeptide chain, while DNAJC2 stimulates its ATPase activity.
Sequence similaritiesBelongs to the heat shock protein 70 family.
Cellular localizationCytoplasm > cytosol.
- Information by UniProt
- Heat shock 70 kDa protein 14 antibody
- Heat shock 70kDa protein 14 antibody
- Heat shock protein HSP60 antibody
All lanes : Anti-HSPA14 antibody [EPR4210] (ab108612) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : COS1 cell lysate
Lane 3 : Human fetal liver lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 55 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of paraffin-embedded Human kidney tissue using ab108612 at 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunofluorescent staining of HeLa cells using ab108612 at 1/100