Anti-HSPA14 antibody [EPR4210] (ab108612)
- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-HSPA14 antibody [EPR4210]
See all HSPA14 primary antibodies -
DescriptionRabbit monoclonal [EPR4210] to HSPA14
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human HSPA14 aa 50-150. The exact sequence is proprietary.
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Positive control
- K562, COS1, Human fetal liver, and HeLa cell lysates. Human kidney tissue. HeLa cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR4210
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IsotypeIgG
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Research areas
Applications
Our Abpromise guarantee covers the use of ab108612 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100 - 1/250. | |
| WB | 1/1000 - 1/10000. Detects a band of approximately 54 kDa (predicted molecular weight: 55 kDa). | |
| IP | 1/10 - 1/100. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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FunctionComponent of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, binds to the nascent polypeptide chain, while DNAJC2 stimulates its ATPase activity.
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Sequence similaritiesBelongs to the heat shock protein 70 family.
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Cellular localizationCytoplasm > cytosol.
- Information by UniProt
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Database links
- Entrez Gene: 51182 Human
- Entrez Gene: 50497 Mouse
- Entrez Gene: 307133 Rat
- Omim: 610369 Human
- SwissProt: Q0VDF9 Human
- SwissProt: Q99M31 Mouse
- SwissProt: Q6AYB4 Rat
- Unigene: 534169 Human
see all -
Alternative names
- Heat shock 70 kDa protein 14 antibody
- Heat shock 70kDa protein 14 antibody
- Heat shock protein HSP60 antibody
see all
Images
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![Western blot - Anti-HSPA14 antibody [EPR4210] (ab108612)](https://www.abcam.com/ps/products/108/ab108612/Images/HSPA14-Primary-antibodies-ab108612-1.jpg)
Western blot - Anti-HSPA14 antibody [EPR4210] (ab108612)All lanes : Anti-HSPA14 antibody [EPR4210] (ab108612) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : COS1 cell lysate
Lane 3 : Human fetal liver lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 55 kDa
Observed band size: 54 kDa (why is the actual band size different from the predicted?) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA14 antibody [EPR4210] (ab108612)](https://www.abcam.com/ps/products/108/ab108612/Images/HSPA14-Primary-antibodies-ab108612-4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA14 antibody [EPR4210] (ab108612)Immunohistochemical analysis of paraffin-embedded Human kidney tissue using ab108612 at 1/100. -
![Immunocytochemistry/ Immunofluorescence - Anti-HSPA14 antibody [EPR4210] (ab108612)](https://www.abcam.com/ps/products/108/ab108612/Images/HSPA14-Primary-antibodies-ab108612-3.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HSPA14 antibody [EPR4210] (ab108612)Immunofluorescent staining of HeLa cells using ab108612 at 1/100
Protocols
References
ab108612 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"