Key features and details
- Rabbit polyclonal to HSPA1L
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HSPA1L antibody
See all HSPA1L primary antibodies
DescriptionRabbit polyclonal to HSPA1L
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig
Recombinant fragment, corresponding to a region within amino acids 335-639 of Human HSPA1L (Uniprot P34931).
- 293T, A431, H1299, HeLaS3, HepG2, Molt4 and Raji whole cell lysates, A431 cells
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154403 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 70 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionIn cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.
Tissue specificityExpressed in spermatids.
Sequence similaritiesBelongs to the heat shock protein 70 family.
- Information by UniProt
- Heat shock 70 kDa protein 1 Hom antibody
- Heat shock 70 kDa protein 1 like antibody
- Heat shock 70 kDa protein 1-Hom antibody
All lanes : Anti-HSPA1L antibody (ab154403) at 1/1000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HeLa S3 whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lane 4 : Molt4 whole cell lysate
Lane 5 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 70 kDa
7.5% SDS PAGE
Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling HSPA1L with ab154403 at 1/500. Image at the bottom costained with Hoechst 33342.
ab154403 has not yet been referenced specifically in any publications.