Anti-HSPA1L antibody (ab154403)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-HSPA1L antibody
See all HSPA1L primary antibodies -
Description
Rabbit polyclonal to HSPA1L -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 335-639 of Human HSPA1L (Uniprot P34931).
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Positive control
- 293T, A431, H1299, HeLaS3, HepG2, Molt4 and Raji whole cell lysates, A431 cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab154403 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 70 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. -
Tissue specificity
Expressed in spermatids. -
Sequence similarities
Belongs to the heat shock protein 70 family. - Information by UniProt
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Database links
- Entrez Gene: 540190 Cow
- Entrez Gene: 3305 Human
- Entrez Gene: 15482 Mouse
- Entrez Gene: 100144518 Pig
- Entrez Gene: 24963 Rat
- Omim: 140559 Human
- SwissProt: P0CB32 Cow
- SwissProt: P34931 Human
see all -
Alternative names
- Heat shock 70 kDa protein 1 Hom antibody
- Heat shock 70 kDa protein 1 like antibody
- Heat shock 70 kDa protein 1-Hom antibody
see all
Images
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Western blot - Anti-HSPA1L antibody (ab154403)All lanes : Anti-HSPA1L antibody (ab154403) at 1/1000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HeLa S3 whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lane 4 : Molt4 whole cell lysate
Lane 5 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 70 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-HSPA1L antibody (ab154403)Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling HSPA1L with ab154403 at 1/500. Image at the bottom costained with Hoechst 33342.
References
ab154403 has not yet been referenced specifically in any publications.
