Anti-HSPA1L antibody (ab154403)
Key features and details
- Rabbit polyclonal to HSPA1L
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HSPA1L antibody
See all HSPA1L primary antibodies -
Description
Rabbit polyclonal to HSPA1L -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 335-639 of Human HSPA1L (Uniprot P34931).
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Positive control
- 293T, A431, H1299, HeLaS3, HepG2, Molt4 and Raji whole cell lysates, A431 cells
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab154403 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 70 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. -
Tissue specificity
Expressed in spermatids. -
Sequence similarities
Belongs to the heat shock protein 70 family. - Information by UniProt
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Database links
- Entrez Gene: 540190 Cow
- Entrez Gene: 3305 Human
- Entrez Gene: 15482 Mouse
- Entrez Gene: 100144518 Pig
- Entrez Gene: 24963 Rat
- Omim: 140559 Human
- SwissProt: P0CB32 Cow
- SwissProt: P34931 Human
see all -
Alternative names
- Heat shock 70 kDa protein 1 Hom antibody
- Heat shock 70 kDa protein 1 like antibody
- Heat shock 70 kDa protein 1-Hom antibody
see all
Images
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Western blot - Anti-HSPA1L antibody (ab154403)All lanes : Anti-HSPA1L antibody (ab154403) at 1/1000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HeLa S3 whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lane 4 : Molt4 whole cell lysate
Lane 5 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 70 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-HSPA1L antibody (ab154403)Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling HSPA1L with ab154403 at 1/500. Image at the bottom costained with Hoechst 33342.
Protocols
References (0)
ab154403 has not yet been referenced specifically in any publications.
