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    hspa1l-antibody-ab154403.pdf

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Signal Transduction Protein Trafficking Chaperones Heat Shock Proteins
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Anti-HSPA1L antibody (ab154403)

  • Datasheet
  • SDS
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Abpromise

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Western blot - Anti-HSPA1L antibody (ab154403)
  • Immunocytochemistry/ Immunofluorescence - Anti-HSPA1L antibody (ab154403)

Key features and details

  • Rabbit polyclonal to HSPA1L
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-HSPA1L antibody
    See all HSPA1L primary antibodies
  • Description

    Rabbit polyclonal to HSPA1L
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 335-639 of Human HSPA1L (Uniprot P34931).

  • Positive control

    • 293T, A431, H1299, HeLaS3, HepG2, Molt4 and Raji whole cell lysates, A431 cells
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Heat Shock Proteins
    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Other Chaperones

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Raji whole cell lysate (ab30124)
    • A431 whole cell lysate (ab7909)
    • MOLT4 whole cell lysate (ab7912)

Applications

Our Abpromise guarantee covers the use of ab154403 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 70 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.
  • Tissue specificity

    Expressed in spermatids.
  • Sequence similarities

    Belongs to the heat shock protein 70 family.
  • Target information above from: UniProt accession P34931 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 540190 Cow
    • Entrez Gene: 3305 Human
    • Entrez Gene: 15482 Mouse
    • Entrez Gene: 100144518 Pig
    • Entrez Gene: 24963 Rat
    • Omim: 140559 Human
    • SwissProt: P0CB32 Cow
    • SwissProt: P34931 Human
    • SwissProt: P16627 Mouse
    • SwissProt: A5A8V7 Pig
    • SwissProt: P55063 Rat
    • Unigene: 690634 Human
    • Unigene: 14287 Mouse
    • Unigene: 187184 Rat
    see all
  • Alternative names

    • Heat shock 70 kDa protein 1 Hom antibody
    • Heat shock 70 kDa protein 1 like antibody
    • Heat shock 70 kDa protein 1-Hom antibody
    • Heat shock 70 kDa protein 1-like antibody
    • Heat shock 70 kDa protein 1L antibody
    • Heat shock 70kD protein like 1 antibody
    • HS71L_HUMAN antibody
    • HSP70 1L antibody
    • HSP70 HOM antibody
    • HSP70-Hom antibody
    • HSPA1L antibody
    • hum70t antibody
    • Spermatid specific heat shock protein 70 antibody
    see all

Images

  • Western blot - Anti-HSPA1L antibody (ab154403)
    Western blot - Anti-HSPA1L antibody (ab154403)
    All lanes : Anti-HSPA1L antibody (ab154403) at 1/1000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : HeLa S3 whole cell lysate
    Lane 3 : HepG2 whole cell lysate
    Lane 4 : Molt4 whole cell lysate
    Lane 5 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 70 kDa



    7.5% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-HSPA1L antibody (ab154403)
    Immunocytochemistry/ Immunofluorescence - Anti-HSPA1L antibody (ab154403)
    Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling HSPA1L with ab154403 at 1/500. Image at the bottom costained with Hoechst 33342.

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab154403? Please let us know so that we can cite the reference in this datasheet.

    ab154403 has not yet been referenced specifically in any publications.

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