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Rabbit polyclonal to HSPA1L
Predicted to work with:
Mouse, Rat, Cow, Pig, Xenopus tropicalis
Recombinant fragment corresponding to a region within amino acids 11-278 of Human HSPA1L (Uniprot ID P34931).
293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates; A540 cells; Human H661 xenograft tissue.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 70 kDa.
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).
1/100 - 1/1000.
In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.
Expressed in spermatids.
Belongs to the heat shock protein 70 family.
Information by UniProt
Heat shock 70 kDa protein 1 Hom antibody
Heat shock 70 kDa protein 1 like antibody
Heat shock 70 kDa protein 1-Hom antibody
Immunocytochemistry/ Immunofluorescence - Anti-HSPA1L antibody (ab154409)
Immunofluorescence analysis of methanol-fixed A549 cells, labeling HSPA1L using ab154409 at a 1/500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA1L antibody (ab154409)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human H661 xenograft tissue, labeling HSPA1L
using ab154409 at a 1/500 dilution.
Western blot - Anti-HSPA1L antibody (ab154409)
Anti-HSPA1L antibody (ab154409) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg
Predicted band size: 70 kDa 7.5% SDS PAGE
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"