Overview

  • Product name
  • Description
    Rabbit polyclonal to HSPA1L
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig, Xenopus tropicalis
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 11-278 of Human HSPA1L (Uniprot ID P34931).

  • Positive control
    • Recombinant Human HSPA1L protein (ab114801) can be used as a positive control in WB. 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates; A540 cells; Human H661 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab154409 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 70 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function
    In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.
  • Tissue specificity
    Expressed in spermatids.
  • Sequence similarities
    Belongs to the heat shock protein 70 family.
  • Information by UniProt
  • Database links
  • Alternative names
    • Heat shock 70 kDa protein 1 Hom antibody
    • Heat shock 70 kDa protein 1 like antibody
    • Heat shock 70 kDa protein 1-Hom antibody
    • Heat shock 70 kDa protein 1-like antibody
    • Heat shock 70 kDa protein 1L antibody
    • Heat shock 70kD protein like 1 antibody
    • HS71L_HUMAN antibody
    • HSP70 1L antibody
    • HSP70 HOM antibody
    • HSP70-Hom antibody
    • HSPA1L antibody
    • hum70t antibody
    • Spermatid specific heat shock protein 70 antibody
    see all

Images

  • Immunofluorescence analysis of methanol-fixed A549 cells, labeling HSPA1L using ab154409 at a 1/500 dilution.
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human H661 xenograft tissue, labeling HSPA1L using ab154409 at a 1/500 dilution.
  • Anti-HSPA1L antibody (ab154409) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size: 70 kDa



    7.5% SDS PAGE

References

This product has been referenced in:
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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