Anti-HSPA1L antibody (ab154409)

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Western blot - Anti-HSPA1L antibody (ab154409)
  • Immunocytochemistry/ Immunofluorescence - Anti-HSPA1L antibody (ab154409)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA1L antibody (ab154409)

Key features and details

  • Rabbit polyclonal to HSPA1L
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-HSPA1L antibody
    See all HSPA1L primary antibodies

  • Description

    Rabbit polyclonal to HSPA1L

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 11-278 of Human HSPA1L (Uniprot ID P34931).

  • Positive control

    • Recombinant Human HSPA1L protein (ab114801) can be used as a positive control in WB. 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates; A540 cells; Human H661 xenograft tissue.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154409 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
WB
Human
All applications
Mouse
Rat
Cow
Pig
Xenopus tropicalis
Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 70 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).
ICC/IF
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 70 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).
ICC/IF
1/100 - 1/1000.

Target

  • Function

    In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.

  • Tissue specificity

    Expressed in spermatids.

  • Sequence similarities

    Belongs to the heat shock protein 70 family.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Heat shock 70 kDa protein 1 Hom antibody
    • Heat shock 70 kDa protein 1 like antibody
    • Heat shock 70 kDa protein 1-Hom antibody
    • Heat shock 70 kDa protein 1-like antibody
    • Heat shock 70 kDa protein 1L antibody
    • Heat shock 70kD protein like 1 antibody
    • HS71L_HUMAN antibody
    • HSP70 1L antibody
    • HSP70 HOM antibody
    • HSP70-Hom antibody
    • HSPA1L antibody
    • hum70t antibody
    • Spermatid specific heat shock protein 70 antibody
    see all

Images

  • Western blot - Anti-HSPA1L antibody (ab154409)
    Western blot - Anti-HSPA1L antibody (ab154409)
    Anti-HSPA1L antibody (ab154409) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size: 70 kDa



    7.5% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-HSPA1L antibody (ab154409)
    Immunocytochemistry/ Immunofluorescence - Anti-HSPA1L antibody (ab154409)
    Immunofluorescence analysis of methanol-fixed A549 cells, labeling HSPA1L using ab154409 at a 1/500 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA1L antibody (ab154409)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA1L antibody (ab154409)
    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human H661 xenograft tissue, labeling HSPA1L using ab154409 at a 1/500 dilution.

References (3)

Publishing research using ab154409? Please let us know so that we can cite the reference in this datasheet.

ab154409 has been referenced in 3 publications.

  • Panagi I  et al. Salmonella Effector SteE Converts the Mammalian Serine/Threonine Kinase GSK3 into a Tyrosine Kinase to Direct Macrophage Polarization. Cell Host Microbe 27:41-53.e6 (2020). PubMed: 31862381
  • Liu T  et al. Genomewide identification and analysis of heat-shock proteins 70/110 to reveal their potential functions in Chinese soft-shelled turtle Pelodiscus sinensis. Ecol Evol 9:6968-6985 (2019). PubMed: 31467669
  • Zhou Y  et al. Simultaneous generation of multi-gene knockouts in human cells. FEBS Lett 590:4343-4353 (2016). PubMed: 27800615

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