Anti-HSPA1L antibody (ab154409)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-HSPA1L antibody
See all HSPA1L primary antibodies -
Description
Rabbit polyclonal to HSPA1L -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig, Xenopus tropicalis
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Immunogen
Recombinant fragment corresponding to a region within amino acids 11-278 of Human HSPA1L (Uniprot ID P34931).
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Positive control
- Recombinant Human HSPA1L protein (ab114801) can be used as a positive control in WB. 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates; A540 cells; Human H661 xenograft tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab154409 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 70 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0). | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. -
Tissue specificity
Expressed in spermatids. -
Sequence similarities
Belongs to the heat shock protein 70 family. - Information by UniProt
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Database links
- Entrez Gene: 540190 Cow
- Entrez Gene: 3305 Human
- Entrez Gene: 15482 Mouse
- Entrez Gene: 100144518 Pig
- Entrez Gene: 24963 Rat
- Entrez Gene: 779883 Xenopus tropicalis
- Omim: 140559 Human
- SwissProt: P0CB32 Cow
see all -
Alternative names
- Heat shock 70 kDa protein 1 Hom antibody
- Heat shock 70 kDa protein 1 like antibody
- Heat shock 70 kDa protein 1-Hom antibody
see all
Images
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Western blot - Anti-HSPA1L antibody (ab154409)Anti-HSPA1L antibody (ab154409) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg
Predicted band size: 70 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-HSPA1L antibody (ab154409)Immunofluorescence analysis of methanol-fixed A549 cells, labeling HSPA1L using ab154409 at a 1/500 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA1L antibody (ab154409)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human H661 xenograft tissue, labeling HSPA1L using ab154409 at a 1/500 dilution.
Protocols
References
This product has been referenced in:
- Liu T et al. Genomewide identification and analysis of heat-shock proteins 70/110 to reveal their potential functions in Chinese soft-shelled turtle Pelodiscus sinensis. Ecol Evol 9:6968-6985 (2019). Read more (PubMed: 31467669) »
- Zhou Y et al. Simultaneous generation of multi-gene knockouts in human cells. FEBS Lett 590:4343-4353 (2016). Read more (PubMed: 27800615) »
