Anti-HSPA1L antibody (ab233153)

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Overview

  • Product name

    Anti-HSPA1L antibody
    See all HSPA1L primary antibodies

  • Description

    Rabbit polyclonal to HSPA1L

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow, Pig, Cynomolgus monkey

  • Immunogen

    Recombinant full length protein (His-tag) corresponding to Human HSPA1L aa 1-641. Expressed in E. coli. N-terminal tag.
    Sequence:

    MATAKGIAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTE RLIGDAAKNQVAMNPQNTVFDAKRLIGRKFNDPVVQADMKLWPFQVINEG GKPKVLVSYKGENKAFYPEEISSMVLTKLKETAEAFLGHPVTNAVITVPA YFNDSQRQATKDAGVIAGLNVLRIINEPTAAAIAYGLDKGGQGERHVLIF DLGGGTFDVSILTIDDGIFEVKATAGDTHLGGEDFDNRLVSHFVEEFKRK HKKDISQNKRAVRRLRTACERAKRTLSSSTQANLEIDSLYEGIDFYTSIT RARFEELCADLFRGTLEPVEKALRDAKMDKAKIHDIVLVGGSTRIPKVQR LLQDYFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSEKVQDLLLLDVAP LSLGLETAGGVMTALIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERA MTKDNNLLGRFDLTGIPPAPRGVPQIEVTFDIDANGILNVTATDKSTGKV NKITITNDKGRLSKEEIERMVLDAEKYKAEDEVQREKIAAKNALESYAFN MKSVVSDEGLKGKISESDKNKILDKCNELLSWLEVNQLAEKDEFDHKRKE LEQMCNPIITKLYQGGCTGPACGTGYVPGRPATGPTIEEVD


    Database link: P34931

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • ICC/IF: HeLa cells. IHC-P: Human testis tissue. WB: Recombinant human HSPA1L protein. HeLa, Raji and HepG2 cell lysate. Rat testis tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab233153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml.
IHC-P Use a concentration of 5 - 20 µg/ml.
ICC/IF Use a concentration of 5 - 20 µg/ml.

Target

  • Function

    In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.

  • Tissue specificity

    Expressed in spermatids.

  • Sequence similarities

    Belongs to the heat shock protein 70 family.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Heat shock 70 kDa protein 1 Hom antibody
    • Heat shock 70 kDa protein 1 like antibody
    • Heat shock 70 kDa protein 1-Hom antibody
    • Heat shock 70 kDa protein 1-like antibody
    • Heat shock 70 kDa protein 1L antibody
    • Heat shock 70kD protein like 1 antibody
    • HS71L_HUMAN antibody
    • HSP70 1L antibody
    • HSP70 HOM antibody
    • HSP70-Hom antibody
    • HSPA1L antibody
    • hum70t antibody
    • Spermatid specific heat shock protein 70 antibody
    see all

Images

  • Western blot - Anti-HSPA1L antibody (ab233153)
    Western blot - Anti-HSPA1L antibody (ab233153)
    Anti-HSPA1L antibody (ab233153) at 1 µg/ml + Rat testis tissue lysate
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA1L antibody (ab233153)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA1L antibody (ab233153)

    Formalin-fixed, paraffin-embedded human testis tissue stained for HSPA1L with ab233153 at 20 µg/ml in immunohistochemical analysis. DAB staining.

  • Immunocytochemistry/ Immunofluorescence - Anti-HSPA1L antibody (ab233153)
    Immunocytochemistry/ Immunofluorescence - Anti-HSPA1L antibody (ab233153)

    HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for HSPA1L using ab233153 at 20 µg/ml in ICC/IF. FITC staining.

  • Western blot - Anti-HSPA1L antibody (ab233153)
    Western blot - Anti-HSPA1L antibody (ab233153)
    Anti-HSPA1L antibody (ab233153) at 1 µg/ml + Raji (Human Burkitt's lymphoma cell line) cell lysate
  • Western blot - Anti-HSPA1L antibody (ab233153)
    Western blot - Anti-HSPA1L antibody (ab233153)
    Anti-HSPA1L antibody (ab233153) at 1 µg/ml + Human HepG2 cell lysate
  • Western blot - Anti-HSPA1L antibody (ab233153)
    Western blot - Anti-HSPA1L antibody (ab233153)
    Anti-HSPA1L antibody (ab233153) at 1 µg/ml + HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
  • Western blot - Anti-HSPA1L antibody (ab233153)
    Western blot - Anti-HSPA1L antibody (ab233153)
    Anti-HSPA1L antibody (ab233153) at 2 µg/ml + Recombinant human HSPA1L protein

References

ab233153 has not yet been referenced specifically in any publications.

Publishing research using ab233153? Please let us know so that we can cite the reference in this datasheet.

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