Overview

  • Product name

  • Description

    Rabbit polyclonal to HSPA2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Chimpanzee, Bovine papillomavirus
  • Immunogen

    Recombinant fragment within Human HSPA2 (internal sequence). The exact sequence is proprietary.
    Database link: P54652

  • Positive control

    • WB: Mouse and rat brain tissue lysates; HEK-293T, A431, NCI-H1299, HeLa, HepG2 and MOLT-4 whole cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab228774 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 70 kDa.

Target

  • Function

    In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.
  • Sequence similarities

    Belongs to the heat shock protein 70 family.
  • Information by UniProt
  • Database links

  • Alternative names

    • 70kDa antibody
    • Hcp70.2 antibody
    • Heat shock 70 kDa protein 2 antibody
    • Heat shock protein 2 antibody
    • Heat shock protein 70.2 antibody
    • Heat shock related 70 kDa protein 2 antibody
    • Heat shock-related 70 kDa protein 2 antibody
    • Heat-shock protein, 70-KD, 2 antibody
    • Heat-shock protein, 70-KD, 3 antibody
    • HSP70 2 antibody
    • HSP70 3 antibody
    • Hsp70-2 antibody
    • HSP70-3 antibody
    • HSP70.2 antibody
    • HSP70A2 antibody
    • HSP72 antibody
    • HSP72_HUMAN antibody
    • HSPA2 antibody
    • Hspt70 antibody
    • Hst70 antibody
    • MGC58299 antibody
    • MGC7795 antibody
    • MGC93458 antibody
    • OTTHUMP00000180664 antibody
    • Testis-specific heat shock protein-related antibody
    see all

Images

  • All lanes : Anti-HSPA2 antibody (ab228774) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Rat brain tissue lysate

    Lysates/proteins at 50 µg per lane.

    Predicted band size: 70 kDa



    7.5% SDS-PAGE gel.

  • All lanes : Anti-HSPA2 antibody (ab228774) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
    Lane 3 : NCI-H1299 (human lung carcinoma cell line) whole cell lysate
    Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 5 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 6 : MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 70 kDa



    7.5% SDS-PAGE gel.

References

ab228774 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228774.
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