Product nameAnti-HSPA2 antibody [EPR4596]
See all HSPA2 primary antibodies
DescriptionRabbit monoclonal [EPR4596] to HSPA2
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment corresponding to Human HSPA2 aa 450-650 (C terminal).
- MCF-7, HeLa, A431, and Jurkat cell lysates; Human colon tissue
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab108416 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended.
FunctionIn cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.
Sequence similaritiesBelongs to the heat shock protein 70 family.
- Information by UniProt
- 70kDa antibody
- Hcp70.2 antibody
- Heat shock 70 kDa protein 2 antibody
All lanes : Anti-HSPA2 antibody [EPR4596] (ab108416) at 1/1000 dilution
Lane 1 : MCF-7 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 70 kDa
ab108416, at 1/100 dilution, staining HSPA2 in paraffin-embedded Human colon tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This product has been referenced in:
- Chen D et al. MicroRNA-634 functions as a tumor suppressor in pancreatic cancer via directly targeting heat shock-related 70-kDa protein 2. Exp Ther Med 17:3949-3956 (2019). Read more (PubMed: 30988777) »
- Ghosh A et al. Chronic E-Cigarette Exposure Alters the Human Bronchial Epithelial Proteome. Am J Respir Crit Care Med 198:67-76 (2018). WB . Read more (PubMed: 29481290) »