Recombinant
Anti-HSPA2 antibody [EPR4596] (ab108416)
- Datasheet
- Specific References (2)
- Protocols
Overview
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Product nameAnti-HSPA2 antibody [EPR4596]
See all HSPA2 primary antibodies -
DescriptionRabbit monoclonal [EPR4596] to HSPA2
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Recombinant fragment corresponding to Human HSPA2 aa 450-650 (C terminal).
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Positive control
- MCF-7, HeLa, A431, and Jurkat cell lysates; Human colon tissue
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR4596
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IsotypeIgG
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Research areas
Applications
Our Abpromise guarantee covers the use of ab108416 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa). | |
| IHC-P | 1/100 - 1/250. Antigen retrieval is recommended. |
Application notes
Is unsuitable for Flow Cyt,ICC or IP.
Target
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FunctionIn cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.
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Sequence similaritiesBelongs to the heat shock protein 70 family.
- Information by UniProt
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Database links
- Entrez Gene: 3306 Human
- Entrez Gene: 15512 Mouse
- Entrez Gene: 60460 Rat
- Omim: 140560 Human
- SwissProt: P54652 Human
- SwissProt: P17156 Mouse
- SwissProt: P14659 Rat
- Unigene: 728938 Human
see all -
Alternative names
- 70kDa antibody
- Hcp70.2 antibody
- Heat shock 70 kDa protein 2 antibody
see all
Images
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![Western blot - Anti-HSPA2 antibody [EPR4596] (ab108416)](https://www.abcam.com/ps/products/108/ab108416/Images/HSPA2-Primary-antibodies-ab108416-1.jpg)
Western blot - Anti-HSPA2 antibody [EPR4596] (ab108416)All lanes : Anti-HSPA2 antibody [EPR4596] (ab108416) at 1/1000 dilution
Lane 1 : MCF-7 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 70 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA2 antibody [EPR4596] (ab108416)](https://www.abcam.com/ps/products/108/ab108416/Images/HSPA2-Primary-antibodies-ab108416-2.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA2 antibody [EPR4596] (ab108416)ab108416, at 1/100 dilution, staining HSPA2 in paraffin-embedded Human colon tissue by Immunohistochemistry.
References
This product has been referenced in:
- Gunnarsson AP et al. Dataset on gene expression profiling of multiple murine hair follicle populations. Data Brief 9:328-34 (2016). Read more (PubMed: 27672671) »
- Nguyen-Lefebvre AT et al. V-erbA generates ribosomes devoid of RPL11 and regulates translational activity in avian erythroid progenitors. Oncogene N/A:N/A (2013). Read more (PubMed: 23563180) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"