RecombinantRabMAb

Recombinant Anti-HSPA2 antibody [EPR4596] (ab108416)

Submit a review Q&A (1)References (8)
Western blot - Anti-HSPA2 antibody [EPR4596] (ab108416)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA2 antibody [EPR4596] (ab108416)
  • Anti-HSPA2 antibody [EPR4596] (ab108416)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4596] to HSPA2
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-HSPA2 antibody [EPR4596]
    See all HSPA2 primary antibodies

  • Description

    Rabbit monoclonal [EPR4596] to HSPA2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment corresponding to Human HSPA2 aa 450-650 (C terminal).

  • Positive control

    • MCF-7, HeLa, A431, and Jurkat cell lysates; Human colon tissue

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR4596

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab108416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.

    • Target

    • Function

      In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.

    • Sequence similarities

      Belongs to the heat shock protein 70 family.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • 70kDa antibody
      • Hcp70.2 antibody
      • Heat shock 70 kDa protein 2 antibody
      • Heat shock protein 2 antibody
      • Heat shock protein 70.2 antibody
      • Heat shock related 70 kDa protein 2 antibody
      • Heat shock-related 70 kDa protein 2 antibody
      • Heat-shock protein, 70-KD, 2 antibody
      • Heat-shock protein, 70-KD, 3 antibody
      • HSP70 2 antibody
      • HSP70 3 antibody
      • Hsp70-2 antibody
      • HSP70-3 antibody
      • HSP70.2 antibody
      • HSP70A2 antibody
      • HSP72 antibody
      • HSP72_HUMAN antibody
      • HSPA2 antibody
      • Hspt70 antibody
      • Hst70 antibody
      • MGC58299 antibody
      • MGC7795 antibody
      • MGC93458 antibody
      • OTTHUMP00000180664 antibody
      • Testis-specific heat shock protein-related antibody
      see all

    Images

    • Western blot - Anti-HSPA2 antibody [EPR4596] (ab108416)
      Western blot - Anti-HSPA2 antibody [EPR4596] (ab108416)
      All lanes : Anti-HSPA2 antibody [EPR4596] (ab108416) at 1/1000 dilution

      Lane 1 : MCF-7 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 70 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA2 antibody [EPR4596] (ab108416)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA2 antibody [EPR4596] (ab108416)

      ab108416, at 1/100 dilution, staining HSPA2 in paraffin-embedded Human colon tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-HSPA2 antibody [EPR4596] (ab108416)
      Anti-HSPA2 antibody [EPR4596] (ab108416)

    References (8)

    Publishing research using ab108416? Please let us know so that we can cite the reference in this datasheet.

    ab108416 has been referenced in 8 publications.

    • Scieglinska D  et al. Various Anti-HSPA2 Antibodies Yield Different Results in Studies on Cancer-Related Functions of Heat Shock Protein A2. Int J Mol Sci 21:N/A (2020). PubMed: 32560263
    • Birket MJ  et al. A Human Stem Cell Model of Fabry Disease Implicates LIMP-2 Accumulation in Cardiomyocyte Pathology. Stem Cell Reports 13:380-393 (2019). PubMed: 31378672
    • Sojka DR  et al. Functional redundancy of HSPA1, HSPA2 and other HSPA proteins in non-small cell lung carcinoma (NSCLC); an implication for NSCLC treatment. Sci Rep 9:14394 (2019). PubMed: 31591429
    • Chen D  et al. MicroRNA-634 functions as a tumor suppressor in pancreatic cancer via directly targeting heat shock-related 70-kDa protein 2. Exp Ther Med 17:3949-3956 (2019). PubMed: 30988777
    • Ghosh A  et al. Chronic E-Cigarette Exposure Alters the Human Bronchial Epithelial Proteome. Am J Respir Crit Care Med 198:67-76 (2018). WB . PubMed: 29481290
    • Dumasia K  et al. Estrogen, through estrogen receptor 1, regulates histone modifications and chromatin remodeling during spermatogenesis in adult rats. Epigenetics 12:953-963 (2017). PubMed: 28949791
    • Gunnarsson AP  et al. Dataset on gene expression profiling of multiple murine hair follicle populations. Data Brief 9:328-34 (2016). PubMed: 27672671
    • Nguyen-Lefebvre AT  et al. V-erbA generates ribosomes devoid of RPL11 and regulates translational activity in avian erythroid progenitors. Oncogene N/A:N/A (2013). PubMed: 23563180

    Customer reviews and Q&As

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    Question

    The datasheet for ab108416 claims that the target of the antibody is HSPA2. To our knowledge, expression of this protein is present only in testes, but the datasheet shows positive IHC signal in colon tissue, where it should not be. Is the data mis-placed, or was the target of the antibody incorrectly specified?

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    Answer

    Thank you for contacting us about antibody ab108416. The ab108416 datasheet does list, as an alternative name for the target of the antibody, "Testis-specific heat shock protein-related antibody". However, the colon data is not misplaced. The antibody was raised against a fragment of human HSPA2 and was in fact validated for IHC on normal human colon tissue.

    I found two recent papers that challenge testes-specific expression, at least for human samples.

    Acta Biochim Pol. 2007;54(1):99-106. Epub 2007 Mar 20. PMID:17369882
    http://www.ncbi.nlm.nih.gov/pubmed?term=17369882

    From the abstract: "HSPA2 is a human counterpart of the testis-specific rodent Hst70/Hsp70.2 gene. In contrast to the latter, the expression of the human HSPA2 gene is not limited to the testis...".

    The same lab published the following tissue microarray study, using their own rabbit polyclonal antibody:

    Histochem Cell Biol. 2011 Apr;135(4):337-50. PMID: 21373891

    http://www.ncbi.nlm.nih.gov/pubmed?term=21373891

    On the other hand, characterization of ab108416 has not been carried out on knockout tissue or on siRNA-treated cells, so cross-reactivity with, for instance, HSPA1 is possible. The western blot data for various lysates shows detection of a single band, but HSPA2 and HSPA1 are the same size.

    I hope this is helpful. Please let me know if you have any questions.

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