Product nameAnti-HSPA4 antibody [EPR14165]
See all HSPA4 primary antibodies
DescriptionRabbit monoclonal [EPR14165] to HSPA4
Tested applicationsSuitable for: Flow Cyt, IP, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human HSPA4 aa 100-200. The exact sequence is proprietary.
Database link: P34932
- K562 cells and cell lysates; HepG2 cell lysate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab185219 in the following tested applications.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IP||1/30 - 1/50.|
|WB||1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 94 kDa).|
Sequence similaritiesBelongs to the heat shock protein 70 family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- APG 2 antibody
- Epididymis secretory sperm binding protein Li 5a antibody
- Heat shock 70 kDa protein 4 antibody
All lanes : Anti-HSPA4 antibody [EPR14165] (ab185219) at 1/5000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 94 kDa
Flow cytometric analysis of 2% paraformaldehyde fixed K562 cells labeling HSPA4 with ab185219 at 1/300 dilution (red) compared with a rabbit monoclonal IgG control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
Western blot analysis of immunoprecipitation pellet from K562 cell lysate labeling HSPA4 using ab185219 at a 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody was used at a 1/1000 dilution.
ab185219 has not yet been referenced specifically in any publications.